Flow cytometry method

Lanier, L.L., and Recktenwald, D.J. (1991) Multicolor immunofluorescence and flow cytometry. Methods... 

Palecanda, A. and Kobzik, L., Alveolar macrophage-environmental particle interaction analysis by flow cytometry, Methods, 21, 241, 2000. 

Metelitsa, L.S., Flow cytometry method for natural killer cells multi-parameter methods for multifunctional cells. Clin. Immunol., 110, 267, 2004. 

In some instances, flow cytometry assays are a superior alternative to conventional procedures for the determination of equilibrium binding constants (Stein et al., 2001). In contrast to assays that employ radiolabelled ligands, which measure population mean values for binding constants, flow cytometry methods can measure those values in individual cells, revealing heterogeneity in receptor expression within a population of cells or membrane vesicles. Furthermore, small samples can be characterized in a short period of time (hours). This approach to receptor-binding analysis may be limited only by the availability of a properly characterized fluorescent ligand. 

Shapiro HM. 2000. Membrane potential estimation by flow cytometry. Methods 21 271-279. 

Darzynkiewicz, Z., and Crissman, H. A. (eds.) (1990) Flow cytometry. Methods Cell Biol. 

Robinson JP, ed. (1993). Handbook of Flow Cytometry Methods. Wiley-Liss/John Wiley and Sons, Inc, New York. An informal, detailed manual of techniques, protocols, and supplies that are relevant to flow cytometric analysis. 

Owing to its applications in homeland security, sterilization validation, and astrobiology, bacterial spore detection has become a hot field. However, direct detection of bacterial spores can be challenging for the same reasons that make endospores difficult to irradicate. The tough spore coat is impermeable to staining techniques, so most microscopy and flow cytometry methods are not useful. Endospores are also highly resistant to lysis, meaning that common DNA extraction protocols are difficult to perform. The lack of measurable metabolism renders microcalorimetry and cellular respiration techniques ineffective. 

Jones RD, Offutt DM, Longmoor BA. Capture ELISA and flow cytometry methods for toxicologic assessment following immunization and cyclophosphamide challenges in beagles. Toxicol Lett 2000 115 33 4. 

Telford WG. Small lasers in flow cytometry. Methods Mol Biol 2004 263 399-418. 

We have combined flow cytometry method, scanning force microscopy and confocal laser scanning microscopy for imaging structural features of leukemic cells from patients with acute lymphoblastic leukemia at different stage of the disease. 

Treatment results in childhood acute leukemia continue to be improved due to new therapeutic and diagnostic methods. An optical microscopy method with specific staining allows to classify acute leukemia into lymphoblastic and myeloid types and discriminate its subtypes according to principals of FAB-classification. A flow cytometry method with monoclonal antibodies staining allows us to discriminate different immunophenotypic subtypes of acute... 

Wang E-J, Casciano CN, Clement RP, Johnson WW. In vitro flow cytometry method to quantitatively assess inhibitors of P-glycoprotein. Drug Metab Dispos 2000 28 522-528. 

Chan, H. E., Jilani, L, Chang, R., Albitar, M. (2007). Detection of chromosome translocations by bead-based flow cytometry. Methods in Molecular Biology, 378, 167—174. Geissmann, F., Jung, S., Littman, D. R. (2003). Blood monocytes consist of two principal subsets with distinct migratory properties. Immunity, 19(1), 71—82. 

Wu, D.Y., Patti-Diaz, L., Hill, C.G. (2010) Development and validation of flow cytometry methods for pharmacodynamic clinical biomarkers. Bioanalysis, 2,1617-1626. 

Stewart CC, Lehnert BE, Steinkamp JA (1986) in vitro and in vivo measurement of phagocytosis by flow cytometry. Methods in Enzym 132 183-192... 

Tsuji, T Sakata, T Dceuchi, Y. Oguni, S. Erythroblast diagnostic flow-cytometry method and reagents. Eur. Pat. Appl. EP 1004880, 2000 Chem. Abstr. 2000, 133, 14302. 

King, M. A. Eddaoudi, A. Davies, D. C. A comparison of three flow cytometry method for evaluating mitochondrial damage during staurosporine-induced apoptosis in Jurkat cells. Cytometry 2007, 71A, 668-674. 

Abe, F. Horikoshi, H. The microorganism detection by the flow cytometry method. Jpn. Kokai Tokkyo Koho JP 2000069995,2000. 

Ito, Y. Lipschitz, D. A. Assay of intracellular hydrogen peroxide generation in activated individual neutrophils by flow cytometry. Methods Mol. Biol. 2002,196,111-116. 

Pina-Vaz, C. Rodrigues, A. G. Evaluation of antifungal susceptibility using flow cytometry. Methods Mol. Biol. 2010, 638, 281-289. 

Guthrie, H. D. Welch, G. R. Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial prohe 5,5 -6,6 -tetrachloro-l, F, 3,3 -tetraethylhenzimidazo-lyl-carbocyanine iodide (JC-1) hy using fluorescence-activated flow cytometry. Methods Mol. Biol. 2008, 477, 89-97. 

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