Matrix-assisted laser desorption/ionization tandem mass

Yao, Z. P. Afonso, C. Fenselau, C. Rapid microorganism identification with on-slide proteolytic digestion followed by matrix-assisted laser desorption/ionization tandem mass spectrometry and database searching. Rapid Comm. Mass Spectrom. 2002,16,1953-1956. 

Li, N., Shaw, A.R., Zhang, N.,Mak,A. andLi, L.(2004)Lipidraftproteomics analysis of in-solution digest of sodium dodecyl sulfate-solubilized lipid raft proteins by liquid chromatography-matrix-assisted laser desorption/ionization tandem mass spectrometry. Proteomics 4, 3156-3166. 

Bienvenut, W.V., C. Deon, C. Pasquarello, J.M. Campbell, J.C. Sanchez, M.L. Vestal and D.F. Hochstrasser. Matrix-assisted laser desorption/ionization-tandem mass spectrometry with high resolution and sensitivity for identification and characterization of proteins. Proteomics 2 868—876, 2002. 

Hirota J, Satomi Y, Yoshikawa K, et al. (2003). e-V,V,V-trimethyIIysine-specific ions in matrix-assisted laser desorption/ionization-tandem mass spectrometry. Rapid Commun. Mass Spectrom. 17 371-376. 

Garrett TJ. Imaging small molecules in tissue by matrix-assisted laser desorption/ ionization tandem mass spectrometry. PhD dissertation. University of Florida, Gainesville, FL, 2006, p. 166. 

Griffiths, W.J. Liu, S. Alvelius, G. SjovaU, J. Derivatisation for the characterisation of neutral oxoster-oids by electrospray and matrix-assisted laser desorption/ ionization tandem mass spectrometry The Girard P derivative. Rapid Commun. Mass Spectrom. 2003, 17 (9), 924-935. 

Matrix-assisted laser desorption/ionization-tandem mass spectrometric method (MALDI-MS/MS) has proven to be a reliable tool for direct measurement of the disposition of small molecules in animal tissue sections. As example, MALDI-MS/MS imaging system was employed for visualizing the spatial distribution of astemizole and its primary metabolite in rat brain tissues. Astemizole is a second-generation antihistamine, a block peripheral HI receptor, which was introduced to provide comparable therapeutic benefit but was withdrawn in most countries due to toxicity risks. Astemizole was observed to be heterogeneously distributed to most parts of brain tissue slices including cortex, hippocampus, hypothalamic, thalamus, and ventricle regions while its major metabolite, desmethylastemizole, was only found around ventricle sites. We have shown that astemizole alone is likely to be responsible for the central nervous system (CNS) side effects when its exposures became elevated. 

Melchior K, Tholey A, Heisel S, Keller A, Lenhof HP, Meese E, et al. Protein versus peptide fractionation in the first dimension of two-dimensional high-performance liquid chromatography—matrix-assisted laser desorption/ionization tandem mass spectrometry for qualitative proteome analysis of tissue samples. J Chromatogr A 2010 1217(40) 6159-68. 

Lattova, E. Perreault, H. Krokmn, O. Matrix-assisted laser desorption/ionization tandem mass spectrometry and post-source decay fragmentation study of phenylhydrazones of N-linked oligosaccharides from ovalbumin. J. Am. Soc. Mass Spectrom. 2004, 15, 725-735. 

W. Wuhrer, C. H. Hokke, and A. M. Deelder, Glycopeptide analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry reveals novel features of horseradish peroxidase glycosylation, Rapid Commun. Mass Spectrom., 18 (2004) 1741-1748. 

Macht, M., Asperger, A., and Deininger, S. O. (2004) Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOE/TOF) for peptide and protein identification. Rapid Commun. Mass Spectrom. 18, 2093-2105. 

Figure 5. Methods employed for identifiying and characterizing proteins separated by 2DE. Abbreviations used 2DE, two-dimensional electrophoresis ESI, electrospray ionization HPLC, high performance liquid chromatography IR, infrared M, relative molecular mass MALDI, matrix-assisted laser desorption ionization MS, mass spectrometry MS/MS tandem mass spectrometry p/, isoelectric point PSD, post-source decay.

Conrotto, R Heilman, U. Lys Tag An easy and robust chemical modification for improved de novo sequencing with a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Rapid Commun. Mass. Spectrom. 2008, 22,... 

APCI, atmospheric pressure chemical ionization Cl, chemical ionization El, electron impact ESI, electrospray ionization FAB, fast atom bombardment GC, gas chromatography LC, liquid chromatography MALDI, matrix-assisted laser desorption ionization MS, mass spectrometry MS/MS, tandem mass spectrometry PAHs, polycyclic aromatic hydrocarbons PEG, polyethylene glycol PPG, polypropylene glycol. 

This chapter describes a step-by-step protocol for top-down proteomic identification of protein biomarkers of bacterial food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture (USDA). 

Lin, M., Campbell, J.M., Mueller, D.R., Wirth, U. (2003) Intact protein analysis by matrix-assisted laser desorption/ ionization tandem time-of-fliglit mass spectrometry. Rapid Commun. Mass Spectrom., 17,1809-1814. 

Alley, W. R., Jr., Mechref, Y., Klouckova, L, Novotny, M. V. (2007) Improved collision-induced dissociation analysis of peptides by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry through 3-sulfobenzoic acid succinimidyl ester labeling. / Proteome Res, 6, 124-132. 

Tandem mass spectrometry (MS/MS) is a method for obtaining sequence and structural information by measurement of the mass-to-charge ratios of ionized molecules before and after dissociation reactions within a mass spectrometer which consists essentially of two mass spectrometers in tandem. In the first step, precursor ions are selected for further fragmentation by energy impact and interaction with a collision gas. The generated product ions can be analyzed by a second scan step. MS/MS measurements of peptides can be performed using electrospray or matrix-assisted laser desorption/ionization in combination with triple quadruple, ion trap, quadrupole-TOF (time-of-flight), TOF-TOF or ion cyclotron resonance MS. Tandem... 

Loboda A.V., Krutchinsky A.N., Bromisrski M., Ens W., and Standing K.G. (2000), A tandem quadrupole/time of flight mass spectrometer with a matrix-assisted laser desorption/ionization source design and performance, Rapid Commun. Mass Spectrom. 14, 1047-1057. 

Yau, P.M. and Burlingame, A.L. (2002) Identification of acetylation and methylation sites of histone H3 from chicken erythrocytes by high-accuracy matrix-assisted laser desorption ionization-time-of-flight, matrix-assisted laser desorption ionization-postsource decay, and nanoelectrospray ionization tandem mass spectrometry. Analytical Biochemistry, 306, 259-269. 

ToF mass spectrometers as dynamic instruments gained popularity with the introduction of matrix assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) as effective pulsed ion sources for the soft ionization of large biomolecules (up to 10s dalton) due to their high ion transmission.38 ToF mass spectrometers, quadrupole analyzers and/or magnetic sector fields can be combined in tandem mass spectrometers (MS/MS) for the analysis of organic compounds. 

Troendle, F. J., Reddick, C. D., and Yost, R. A. (1999). Detection of pharmaceutical compounds in tissue by matrix-assisted laser desorption/ionization and laser desorption/chemi-cal ionization tandem mass spectrometry with a quadruple ion trap. J. Am. Soc. Mass Spectrom. 10 1315-1321. 

---