Microorganisms, identification

Maier, T., Klepel, S., Renner, U., and Kostrzewa, M. (2006). Fast and reliable MALDl-TOF MS-based microorganism identification. Nat. Methods 3, 1-2. 

Pineda, F. J. Lin, J. S. Fenselau, C. Dimerov, P. A. Testing the significance of microorganism identification by mass spectrometry and proteome database search. Anal. Chem. 2000, 72, 3739-3744. 

Valentine, N. B. Wunschel, S. C. Petersen, C. E. Wahl, K. L. The effect of culture conditions on microorganism identification by MALDI mass spectrometry. Appl. Environ. Microbiol., 2005, 71, 58-64. 

An algorithm has been developed to search databases for suites of protein masses automatically (Pineda et al.).65 In this case data from only one axis is being used, m/z values for the protonated molecular ions. Relative intensities are not used in the analysis. The algorithm also assesses the probability of accidental matches and false identifications. Significance testing allows a value to be assigned to each microorganism identification. 

Demirev, R A. Feldman, A. B. Lin, J. S. Bioinformatics-based strategies for rapid microorganism identification by mass spectrometry. JHU-APL Tech Dig. 2004,25, 27-37. 

Yao, Z. P. Afonso, C. Fenselau, C. Rapid microorganism identification with on-slide proteolytic digestion followed by matrix-assisted laser desorption/ionization tandem mass spectrometry and database searching. Rapid Comm. Mass Spectrom. 2002,16,1953-1956. 

Microorganisms—Identification. 2. Mass spectrometry. I. Wilkins, Charles L. 

Among analytical techniques, the pyrolytic procedures can be applied for microorganism identification due to their sensitivity, need for a minute amount of sample, applicability to a wide range of microorganisms, and the relatively short time required for analysis [1]. Several problems are related to the use of pyrolytic techniques for microorganism characterization. Some are related to sample preparation and are not specific to pyrolytic procedures, while others are related to the pyrolytic technique itself. 

Pineda, F.I, Antoine, M.D., Demirev, P.A., Feldman, A.B., Jackman, ,Longenecker,M.,Lin,IS. (2003) Microorganism identification by matrix-assisted laser/desorption ionization mass spectrometry and model-derived ribosomal protein biomarkers. Analytical Chemistry, 75, 3817-3822. 

Valentine, N., Wnnschel, S., Wnnschel, D., Petersen, C., Wahl, K. (2005) Effect of cnltnre conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry. Applied and Environmental Microbiology, 71,58-64. 

Demirev PA, Lin JS, Pineda FJ, Fenselau C. Bioinformatics and mass spectrometry for microorganism identification proteome-wide post-translational modifications and database search algorithms for characterization of Intact H. Pylori. Anal Chem. 2001 73(19) 4566-73. 

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