Succinimidyl ester

Polymerization of a stable intermediate prepared from two monomers can produce an alternating copolymer. The amino acid azide hydrobromide method and the amino acid succinimidyl ester hydromide method were applied to prepare ordered copolyamides277) and sequential copolypeptides278-280). 

Lysine Acid anhydrides, succinimidyl esters, iso thiocyanates, trinitrobenzenesulfonic acid... 

Following previous work, Hagiwara and collaborators [71] recently prepared 5 -terminal acridone-labeled DNAs, using the succinimidyl ester 24 of the acridone acetic acid 23 reported before [69], and evaluated their use as donors for a fluorescence resonance energy transfer (FRET) system in combination with 3 -dabcyl-tagged DNA... 

Tian and co-workers [148] have reported the synthesis of two chlorinated fluoresceins probes 4,7,2,7 -tetrachloro-6-(5-carboxypentyl) fluorescein and 4,7,4,5 -tetrachloro-6-(5-carboxypentyl) fluorescein for labeling proteins. More recently, the same research group has described the synthesis of two novel chlorinated fluoresceins, namely 2,4, 5, 7 -tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester 52 and 2, 4, 5, 7-tetrachloro-6-(3-carboxypropyl)-4,7-dichlorofluorescein succinimidyl ester 53 [149]. 

The novel chlorinated fluorescein succinimidyl esters 52 and 53 are considerably stable if properly stored. They exhibit intermediate reactivity toward amines, with high selectivity toward aliphatic amines. Their reaction rate with aromatic amines, alcohols, phenols, and histidine is relatively low. 

Mujumdar RB, Ernst LA, Mujumdar SR, Lewis CJ, Waggoner AS (1993) Cyanine dye labeling reagents sulfoindocyanine succinimidyl esters. Bioconjugate Chem 4 105-111... 

Mujumdar SR, Mujumdar RB, Grant CM, Waggoner AS (1996) Cyanine-labeling reagents sulfobenzindocyanine succinimidyl esters. Bioconjugate Chem 7 356-362... 

An important factor in all these experiments is the choice of bead used to immobilize the probe. Biochemists have considered cross-linked agarose beads to be exceptionally hydrophilic with a low tendency to bind proteins nonspecifically, and these beads have the further attraction of being commercially available in activated forms (succinimidyl esters, epoxides, and maleimides, for example). However, early trials of bead-based chemical proteomics have shown that many proteins in mammalian cell lysates bind tenaciously to agarose beads. This was unimportant in many studies in which protein-protein interactions were detected by coimmunoprecipitation with immunochemical... 

Dabcyl-N-succinimidyl ester aminofluorescein Texas Red hydrazide... 

Use of sulfo-NHS-LC-SPDP or other heterobifunctional crosslinkers to modify PAMAM dendrimers may be done along with the use of a secondary conjugation reaction to couple a detectable label or another protein to the dendrimer surface. Patri et al. (2004) used the SPDP activation method along with amine-reactive fluorescent labels (FITC or 6-carboxytetramethylrhodamine succinimidyl ester) to create an antibody conjugate, which also was detectable by fluorescent imaging. Thomas et al. (2004) used a similar procedure and the same crosslinker to thiolate dendrimers for conjugation with sulfo-SMCC-activated antibodies. In this case, the dendrimers were labeled with FITC at a level of 5 fluorescent molecules per G-5 PAMAM molecule. 

NHS-rhodamine is an amine-reactive fluorescent probe that contains a carboxy-succinimidyl ester group off the No. 5 or 6 carbons on rhodamine s lower-ring structure (Kellogg et al., 1988). The 5- and 6-isomers are virtually identical in their reactivity and fluorescent characteristics. Similar to TRITC (described previously), NHS-rhodamine can be used to label proteins and other macromolecules that contain primary amine groups. The isomeric forms of the fluorescent probe are available in mixed and purified forms (Invitrogen, Thermo Fisher). The pure forms are... 

BODIPY FL C3-SE is 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, succinimidyl ester (Invitrogen). The derivatization to the base BODIPY molecule... 

Active ester stock solution A dissolvelO mg of 5-(and-6)carboxyfluorescein, succinimidyl ester (FITC-NHS, Molecular Probes -1311) in 1 ml of DMF. 

NL Benoiton, FMF Chen. A-9-Fluorenylmethoxycarbonylpyroglutamate. Preparation of the acid, chloride and succinimidyl ester. Int J Pept Prot Res 43, 321, 1994. 

NL Benoiton, YC Lee, FMF Chen. A new coupling method allowing epimerization-free aminolysis of segments. Use of succinimidyl esters obtained through mixed anhydrides, in HLS Maia, ed. Peptides 1994. Proceedings of the 23rd European Peptide Symposium, Escom, Leiden, 1995, pp 203-204. 

J Savrda. An unusual side reaction of 1-succinimidyl esters during peptide synthesis. J Org Chem 42, 3199, 1977. 

Fig. 5.12 On-line continuous-flow monitoring of bioactive compounds using fluorescein-biotin/streptavidin assay. MS instrument Q-ToF2 (Waters) equipped with a Waters Z-spray electrospray (ESI) source. Triplicate injections of (a) biotin-N-succinimidyl ester (m/z 342), (b) N-biotinyl-L-lysine (m/z 373),...

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