Matrix assisted laser desorption/ionization linear

Brown, R. S. Lennon, J. J. Mass resolution improvement by incorporation of pulsed ion extraction in a matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometer. Anal. Chem. 1995,67,1998-2003. 

R. S. Brown and J. J. Lennon. Mass Resolution Improvement by Incorporation of Pulsed Ion Extraction in a Matrix-Assisted Laser Desorption/Ionization Linear Time-of-Flight Mass Spectrometer. Anal. Chem., 67(1995) 1998-2003. 

Bahr, U., StahlZeng, J., Gleitsmann, E. and Karas, M., Delayed extraction time-of-flightMALDI mass spectrometry of proteins above 25,000 Da. J. Mass Spectrom., 32, 1111-1116 (1997). Brown, R.S. and Lennon, J.J., Mass resolution improvement by incorporation of pulsed ion extraction in a matrix-assisted laser-desorption ionization linear time-of-flight mass-spectrometer. AwaZ. Chem., 67, 1998-2003 (1995). 

R. M. Whittal, L. M. Russon, S. R. Weinberger, and L. Li. Functional Wave Time-Lag Focusing Matrix-Assisted Laser Desorption/Ionization in a Linear Time-of-Flight Mass Spectrometer Improved Mass Accuracy. Anal Chem., 69(1997) 2147-2153. 

Linear energy transfer Locally multiply damaged sites Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry... 

Kemptner J (2009) Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 23 877-884. doi 10.1002/... 

Liquid Secondary Ion Mass Spectrometry Linear Time of Flight Matrix-Assisted Laser Desorption Matrix-Assisted Laser Desorption/Ionization Molecular Beam... 

The methods for each study are divided into the initial protein separation step, a second separation step if applicable, the type of mass analysis, and the software used for peptide identification. ID = one dimensional polyacrylamide gel electrophoresis, 2D = two dimensional polyacrylamide gel electrophoresis, MS = mass spectrometry (peptide mass fingerprinting), MS/MS = tandem mass spectrometry, MALDI-TOF = matrix assisted laser desorption/ionization-time of flight, MS FIT = software from Protein Prospector, http //prospector.ucsf edu/, ESI = electrospray ionization, Q-TOF = quadrupole-time of flight, PPSS2 =Protana s Proteomic Software Suite (ProtanaEngineering, Odense, Denmark), Mascot = Matiix Science, http //www.matrixscience.com/, TOF-TOF = MALDI plus TOF tandem mass spectrometry, RP-HPLC = reverse phase high performance liquid chromatography, Q-IT = quadrupole ion trap, LIT = linear ion trap. Bioworks = Thermo Electron Corporation. 

Matrix Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS) Mass spectra of native and denatured antibodies were obtained with a PerSeptive Biosystems (Farmingham, MA.) Voyager Elite mass spectrometer operated in the linear mode with a Laser Sciences Inc., 337 nm nitrogen laser. hAB-1 was denatured by boiling the sample in 1.0 M guanidine-HCl, 50 mM Tris pH 7.5 buffer. Native and denatured samples were diluted with 20 mM Tris, 10 mM octylglucoside (Tris/OG) pH 6.8 buffer prior to MALDl-MS analysis. Proteins were spotted on the sample plate as a sandwich between two layers of the matrix. The bottom layer consisted of 100 mM sinapinic acid in acetonitrile and the top layer consisted of 50 mM sinapinic acid in 30% acetonitrile / 70% H2O / 0.07% TFA. The m/z scale of the instrument was calibrated using a Hewlett-Packard protein standard mixture. 

Kaufmann, R., Spengler, B., and Lutzenkirchen, R, Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization. Rapid Communication Mass Spectrometry, 7, 902-910, 1993. 

Leon, J. W. and Fr het, J. M. J., Analysis of Aromatic Polyether Dendrimers and Dendrimer-linear Block Copolymers by Matrix-assisted Laser Desorption Ionization Mass Spectrometry, Polym. Bull., 35, 449,1995. 

Typically, MALDl has been performed under low pressure conditions (ca 10 Torr). In recent years, intermediate pressure (IP, 10 to 1 Torr) [10,12-14] and atmospheric pressure (AP) MALDl [15,16] systems have been developed to analyze more labile molecules and for tissue analysis [10]. Atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDl) offers the ability to couple a MALDl source to most commercial instruments, including quadrupole ion traps, because the source uses existing ion optics and vacuum transfer lines of commercial instruments to introduce the ions generated by MALDL A brief discussion of IP-MALDI with ion trap technology is presented, but the primary focus is on the instrumentation aspect of ion trap technology, in particular linear ion trap technology, for direct tissue analysis and imaging MS studies. 

J. Krause, M. Scalf, and L. M. Smith, High resolution characterization of DNA fragment ions produced by ultraviolet matrix-assisted laser desorption/ionization using linear and reflecting time-of-flight mass spectrometry, J. Am. Soc. Mass Spectrom. 10, 423-429, (1999). 

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