PerSeptive Biosystems

Figure 4.12 On-line coupling of the bifur-cation-distributive chip micro mixer to a Perseptive Biosystems Mariner TOF-MS [25],...

Y Yao, TA Taylor, M Fuchs. Unpublished data. PerSeptive Biosystems. Framingham, MA, 1996. 

HATU was purchased from PerSeptive BioSystem, GmbH. 

Matrix-Assisted Laser Desorption/lonization Time-of-liight Mass Spectrometry (MALDI-TOF MS, PerSeptive Biosystems Voyager-DE STR) was used to characterize the molecular weight distribution and the number average molecular weight (M ) of the poly(methacrylic acid) (PMAA) degradation products as described elsewhere [25]. 

Matrix assisted laser desorption ionization time-of-flight (MALDI-TOE) mass spectrometry was carried out with a PerSeptive Biosystems Voyager-DE-RP MALDl-TOF mass spectrometer. A 337-nm UV nitrogen laser producing 3-ns pulses was used in the reflectron mode. The samples were prepared by mixing 10 pi of a 0.1 M HAc solution of the sample with 20 pi of a solution of 3 mg/1 a-cyano-4-hydroxy cinnamic acid in wafer. One pi of that solution was loaded on the gold-sample plate. 

Transport of biomolecules through chromatographic media. A Conventional support particles. B POROS particles for perfusion chromatography. Courtesy of PerSeptive Biosystems, Cambridge, MA,... 

S. Fulton and D. Vanderburgh, The Busy Researcher s Guide to Biomolecule Chromatography (1996), PerSeptive Biosystems (Framingham, MA). Practical guide to chromatography of biomolecules with emphasis on perfusion methods. 

Bal resin (0.4 g, 0.45 mmol/g, 0.18 mmol, Perseptive Biosystems) was weighed into a fritted polypropylene tube and swelled with 4 ml of DCM. To the suspension was added oxazolidinone HC1 salt 2 (96 mg, 0.27 mmol), sodium triacetoxyborohydride (190 mg, 0.90 mmol), and acetic acid (80 pi,... 

PerSeptive Biosystems Pores polymeric aggregates Protein A, trypsin... 

The MALDI/TOF mass spectrum of a 25-mer oligonucleotide after enzymatic digestion with a type I phosphodiesterase at three different concentrations. The different peaks correspond to successive cleavages from the 3 side to the 5 one. Reproduced (modified) from a PerSeptive biosystems documentation, with permission. 

MALDI-TOF Mass Spectrometry. Peptide/detergent mixtures as well as purified peptides were analyzed using a Vestec LaserTec BenchTop II system (PerSeptive Biosystems). Peptides were mixed with alpha-cyano-4-hydroxycinnamic acid as previously described (4). 

MALDI-TOF mass spectrometric analysis. This is currently being addressed by Vestec/PerSeptive Biosystems. 

Present address PerSeptive Biosystems, Inc., 38 Sidney Street, Cambridge MA 02139... 

PerSeptive Biosystems Pioneer Fmoc 0.020 - 2.0 automatic, 2 columns... 

Pioneer Multiple Peptide Synthesis Accessory Users Guide, PerSeptive Biosystems, Inc. Framingham, (1997) p Al. 

Matrix Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS) Mass spectra of native and denatured antibodies were obtained with a PerSeptive Biosystems (Farmingham, MA.) Voyager Elite mass spectrometer operated in the linear mode with a Laser Sciences Inc., 337 nm nitrogen laser. hAB-1 was denatured by boiling the sample in 1.0 M guanidine-HCl, 50 mM Tris pH 7.5 buffer. Native and denatured samples were diluted with 20 mM Tris, 10 mM octylglucoside (Tris/OG) pH 6.8 buffer prior to MALDl-MS analysis. Proteins were spotted on the sample plate as a sandwich between two layers of the matrix. The bottom layer consisted of 100 mM sinapinic acid in acetonitrile and the top layer consisted of 50 mM sinapinic acid in 30% acetonitrile / 70% H2O / 0.07% TFA. The m/z scale of the instrument was calibrated using a Hewlett-Packard protein standard mixture. 

Tryptic mapping was performed on an HP 1090 HPLC modified with two independent switching valves for controlling flow to a Poroszyme immobilized trypsin Cartridge (Perseptive Biosystems) and an in-line Zorbax analytical C18 column (Lippincott et al., manuscript in preparation). 

Saskia C.F. Milton, R.C. de Lisle Milton, Steven A. Kates and Charles Glabe Department of Molecular Biology and Biochemistry, University of California, Irvine CA 92697 Advanced Technology Center, Beckman Instruments Inc., Fullerton CA 92634 PerSeptive Biosystems Inc., Framingham MA 01701... 

Side chain protected Fmoc amino acids, Fmoc-L-Ala-PEG-polystyrene support, BOP and HATU were obtained from PerSeptive Biosystems, Inc. DAST (diethylaminosulfur trifluoride) was obtained from Fluka Chemical Corp. and DIEA and NMM were obtained from Aldrich Chemical Co., Inc. The following Fmoc amino acid derivatives were fluorinated Ala, Phe, Gly, He, Leu, Met, Val, Asp(OfBu), Glu(OfBu), Lys(Boc), Asn(Trt), Gln(Trt), Ser(fBu) and Tyr(0/Bu), while His(Trt) and Arg(Pbf) were used as purchased. All solvents were of HPLC grade and all chemicals of Analar grade. DMF was dried over 4 Angstrom sieve before use. 

Perkin-Elmer Corporation, Applied Biosystems Division PerSeptive Biosystems, Inc. Pharmacia Biosensor Pharmacia Biotech, Inc. 

PerSeptive Biosystems (Framingham, MA) 96-well fluorescence plate reader with a 320 20 nm excitation filter (made by reversing the respective emission filter) and a 420 20 nm emission filter (or any other equivalent fluorescence plate reader and filters). 

ID, Immunodetection, Real-Time Immunoassay. Cambridge, MA PerSeptive Biosystems Technical Literature, 1994. 

PerSeptive Biosystems Technical Operating Instructions, ImmunoDetection Sensor Cartridge. Cambridge, MA PerSeptive Biosystems, 1992. 

Profile The company was founded in 1993. PerSeptive Biosystems is one of its principal investors. In January 1997 it was jointly announced that Millennium Pharmaceuticals would acquire privately held ChemGenics Pharmaceuticals as part of their mutual strategy to become a world leader in drug discovery. Following the merger, the combined company will employ more than 345 people. PerSeptive Biosystems is also a principal ChemGenics shareholder. In August 1997, Perkin-Elmer announced its plans to acquire PerSeptive. 

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