In vitro biocompatibility

Nadagouda, M.N., Castle, A.B., Murdock, R.C., Hussain, S.M. and Varma, R.S. (2010) In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols. Green Chemistry, 12, 114-122. 

The tests for in vitro biocompatibility were performed on normal cells (Vero) and tumor cells (glioblastoma). No cytotoxicity was detected in cells (normal or tumor) cultured with red and white onion extracts at low concentrations (between 0% and 1%), the cell viability being aronnd 90%. At higher concentration, the viability decrease slowly at 80% (Fig. 41.4a). On the other hand, garlic extracts indnce toxicity both on normal and tnmor cells at very low concentrations (Fig. 41.4b). 

Sangsanoh P et al (2007) In vitro biocompatibility of schwattn cells on surfaces of biocompatible polymeric electrospun fibrous and solution-cast film scaffolds. Biomacromolecules 8 (5) 1587-1594... 

Hoppe, A., Will,., Detsch, R., Boccaccini, A.R., and Greil, P. (2013) Formation and in vitro biocompatibility of biomimetic hydroxyapatite coatings on chemically treated carbon substrates. / Biomed. 

The Canine Model. While ex vivo models often are considered to be an improvement over in vitro biocompatibility test systems, the problem of describing extremely complex blood—polymer interactions still remains. In this study, we used radioisotope-labeled proteins and platelets and scanning electron microscopy. In other studies, we applied immunolabeling techniques and transmission electron microscopy. The application of these tools to an in vivo or ex vivo system provides more pertinent data than that often obtained in an in vitro system. Through this approach we hope to gain some insights into the complicated interactions of blood with biomaterials. 

Beumer GJ, Van Blitterswijk CA, Bakker D, Ponec M. Cell-seeding and in vitro biocompatibility evaluation of pol3uneric matrices of PEO/PBT copolymers and PTJ.A. Biomaterials 1993 14 598-604. 

Qu, X. H. Wu, Q. Liang, J. Zou, B. and Chen, G. Q. Effect of 3-hydroxyhexanoate content in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) on in vitro growth and differentiation of smooth muscle cells. Biomater. 2006 May, 27(15), 2944-2950. Sangsanoh, R et al. In vitro biocompatibility of schwann cells on surfaces of biocompatible polymeric electrospun fibrous and solution-cast film scaffolds. Biomacromole. 2007, 8(5), 1587-1594. 

Suwantong, O. Waleetomcheepsawat, S. Sanchavanakit, N. Pavasant, R Cheep-sunthom, P Bunaprasert, T Supaphol, R In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats. Int. J. BiolMacromol. 2007, 40(3), 217-23. 

Chanvel-Lesrat, D. J. Pellen-Mussi, P Auroy, P and Bonnaure-Mallet, M. Evaluation of the in vitro biocompatibility of various elastomers. Biomater. 1999, 20, 291-299. 

Ignatius, A. A., Claes, L. E. In vitro biocompatibility of bioresorbable polymers poly(l, dl-lactide) and poly(l-lactide-co-glycolide). 1996,17(8), 831-839. 

The in vitro biocompatibility of CPs has been generally investigated by cell viability, proliferation and cytotoxicity assays of various cell types, including rat pheochromocy-toma cells (PC 12), rat Schwann cells, cardiac myoblasts, astrocytes, and various neural tissue explants [ 13,27,43,47]. Studies on a wide variety of CPs have indicated good in vitro cell responses, with minimal cytotoxicity and, in several cases, preferential adherence of cells to the CP surface, compared to conventional implant materials. However, the majority in vitro studies examine the passive or nonelectrochemically activated state of CPs. Due to the application-specific nature of biocompatibility, passive CP assessment, although a useful preliminary study, is an imperfect characterization of CP biological performance for most implant applications. 

Sannino, A. Madaghiele, M. Lionetto, M.G. Schettino, T. Maffezzoli, A. A cellulose-based hydrogel as a potential bulking agent for hypocaloric diets An in vitro biocompatibility study on rat intestine. J. Appl. Polym. Sci. 2006,102 (2), 1524-1530. 

Electrospinning of PHA is still relatively new in scaffold fabrication. To date, P(3HB) and P(3HB-co-3HV) are the most common microbial polyesters to be electrospun into tissue-engineering scaffolds. Suwantong et al. (2007) prepared ultrafine electrospun fiber mats of P(3HB) and P(3HB-co-3HV) as scaffolding materials for skin and nerve generation, hi their study, they evaluated the in vitro biocompatibility of these fibers using mouse fibroblasts and Schwann cells... 

M. Pergal,V. Antid, G. Tovilovic, J. Nestorov, D.Vasiljevid-Radovid, J. Djonlagid, In vitro biocompatibility evaluation of novel urethane-siloxane co-polymers based on poly(e-caprolactone)-block-poly(dimethylsiloxane)-block-poly(e-caprolactone), J. Biomater. Sci. Polym. Ed. 23 (13) (2012) 1629-1657. 

M.V. Risbud, R.R. Bhonde, Suitability of cellulose molecular dialysis membrane for bioartificial pancreas in vitro biocompatibility studies, J. Biomed. Mater. Res. 54 (3) (2001) 436 44. 

Zhang, Z., R. Roy, F.J. Dugre, D. Tessier, and L.H. Dao. 2001. In vitro biocompatibility study of electrically conductivepolypyrrole-coated polyester fabrics. / Biomed Mater Res 57 63. 

Amaral M, Dias AG, Gomes PS, Lopes MA, Silva RF (2008) Nanocrystalline Diamond In Vitro Biocompatibility Assessment by MG63 and Human Bone Marrow Cells Cultures. J. biomater, res. A 87 91-99. 

Salcedo 1, Aguzzi C, Sandri G, Bonferoni MC, Mori M, Cerezo P, Sanchez R, Viseras C, Caramella C (2012) In vitro biocompatibility and mucoadhesion of montmorillonite chitosan nanocomposite a new drug delivery. Appl Clay Sci 55 131-137... 

Morrison C, Macnair R, MacDonald C, Wykman A, Goldie I, Grant MH (1995), In vitro biocompatability testing of polymers for orthopaedic implants using cultured fibroblasts and osteoblasts . Biomaterials, 16, 987-992. 

Injectable bone substitute material consisting of CTS, citric acid, and glucose solution as the liquid phase, and tricalcium phosphate powder as the solid phase, was developed by Liu and coworkers [141]. Four types of cements have been used to investigate the mechanical properties and in vitro biocompatibility of the material. In the presence of citric acid, tricalcium phosphate partially transformed into HAp and dicalcium phosphate. 

Shen, X., Su, F., Dong, J., Fan, Z., Duan, Y., Li, S., 2015. In vitro biocompatibility evaluation of bioresorbable copolymers prepared from L-lactide, 1, 3-trimethylene carbonate, and gly-cobde for cardiovascular appbcations. Journal of Biomaterials Science Polymer Edition 26, 497-514. 

In vitro biocompatibility tests were also performed by Neenan et al. (1982). They used heparinized poly[(organo)phosphazenes] to enhance the anti-thrombogenic properties of polyphosphazenes. Films of these materials containing heparine were subjected to bovine blood clotting tests (Figure 29). 

Hsu SH, Tseng HJ. In vitro biocompatibility of PTMO-based polyurethanes and those containing PDMS blocks. J Biomater Appl 2004 19(2) 135-46. 

Park JH, Bae YH. Physicochemical properties and in vitro biocompatibility of PEG/ PTMO multiblock copolymer/segmented polyurethane blends. J Biomater Sci Polym Ed 2002 13(5) 527-42. 

Albanese A, Barbucci R, Belleville J, et al. In vitro biocompatibility evaluation of a hep-arinizable material (PUPA), based on polyurethane and poly(amido-amine) components. Biomaterials January 1994 15(2) 129-36. 

Liang, S.L., Cook, W.D., Thouas, G.A., Chen, Q.Z., 2010. The mechanical characteristics and in vitro biocompatibility of poly(glycerol sebacatej-Bioglass elastomeric composites. Biomaterials 31, 8516-8529. 

Guelcher SA, et al. Synthesis and in vitro biocompatibility of injectable polyurethane foam scaffolds. Tissue Eng 2006 12(5) 1247—59. 

Kim, TN Balakrishnan, A Lee, BC Kim, WS Smetana, K Park, JK Panigrahi, BB. In vitro biocompatibility of equal channel angular pressed (ECAP) titanium. Biomedical Materials, 2007, 2, S117-S120. 

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