Hybrid quadrupole

Chapter 23 Hybrid Quadrupole Time-of-Flight (Q/TOF) Instruments... 

Farre M, Perez S, Gajda-Schrantz K, Osorio V, Kantiani L, Ginebreda A, Barcelo D (2010) First determination of C60 and C70 fullerenes and N-methylfulleropyrrolidine C60 on the suspended material of wastewater effluents by liquid chromatography hybrid quadrupole linear ion trap tandem mass spectrometry. J Hydrol 383(l-2) 44-51... 

The instrumental analysis for the identification of UV filters degradation products formed during the fungal treatment process was performed by means of HPLC coupled to tandem mass spectrometry using a hybrid quadrupole-time-of-flight mass spectrometer (HPLC-QqTOF-MS/MS). Chromatographic separation was achieved on a Hibar Purospher STAR HR R-18 ec. (50 mm x 2.0 mm, 5 pm, from Merck). In the optimized method, the mobile phase consisted of a mixture of HPLC grade water and acetonitrile, both with 0.15% formic acid. The injection volume was set to 10 pL and the mobile phase flow-rate to 0.3 mL/min. 

New instrumentation for the analysis of the proteome has been developed including a MALDI hybrid quadrupole time of flight instrument which combines advantages of the mass finger printing and peptide sequencing methods for protein identification (Andersen and Mann 2000). 

Weaver, P. J., Laures, A. M. F., and Wolff, J. C. (2007). Investigation of the advanced functionalities of a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer. Rapid Commun. Mass Spectrom. 21 2415-2421. 

Wrona, M., Timo, M., Bateman, K. P., Mortishire-Smith, R. J., and O Connor, D. (2005). All-in-one analysis for metabolite identification using liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry with collision energy switching. Rapid... 

Serum Modified Blight and Dyer extraction Micro HPLC (Ci 8) MS ESI hybrid quadrupole time of flight 58 (36)... 

Among these, both LC-MS and LC-MS/MS approaches have been described using different mass analyzers operating in the positive ion mode scan such as triple stage quadrupole (TSQ) mass spectrometers [145, 194, 226, 227, 235, 239, 240, 242-247] and hybrid quadrupole-linear ion trap (LTQ) [173, 193, 218, 238, 241] mass spectrometers working in SRM mode as well as time-of-flight (TOF) [195] and hybrid quadrupole-TOF (Q-TOF) [236,237] mass spectrometers working in the MS mode. 

Taylor K, Elliott S (2009) A validated hybrid quadrupole linear ion-trap LC-MS method for the analysis of morphine and morphine glucuronides applied to opiate deaths. Forensic Sci Int 187(l-3) 34 fl. doi S0379-0738(09)00085-l [pii] 10.1016/j.forsciint.2009.02.011... 

The ability of this ionization method for the determination of very high molecular weights is illustrated in Figure 1.26 [68], The spectrum displayed is obtained from assemblies of vanillyl alcohol oxidase containing respectively 16 and 24 proteins. The spectrum was obtained with a hybrid quadrupole TOF instrument, Q-TOF Micromass, equiped with a micro-ESI source. To obtain such a spectrum one needs not only a mass spectrometer with sufficient mass range and resolution, but also high skill in protein purification. 

Hoyes, J. B., Jones, C., Langridge, J. I., Miliar, A., Vissers, J. P. (2002). A novel precursor ion discovery method on a hybrid quadrupole orthogonal acceleration time-of-flight (Q-TOF) mass spectrometer for studying protein phosphorylation. 

Ekroos K, Chernushevich IV, Simons K, Shevchenko A. Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer. Anal Chem. 2002 74 941-949. 

In terms of mass spectrometry instrumentation, the currently available instruments such as time-of-flight (TOF) analyzers and hybrid quadrupole-TOF analyzers are able to acquire complete mass spectra at rates compatible with fast CE separations. As CE or ultrafast chromatography replaces conventional, slow HPEC applications, TOF-based mass spectrometers will be needed to replace the less efficient scanning types of instruments such as quadrupoles and ion traps for most high-throughput applications. FTICR mass spectrometry remains unsurpassed in terms of resolution and mass accuracy for both MS and MS-MS applications. However, the throughput of FTICR mass spectrometric... 

The need to detect lower concentration of protein and peptide mixtures has resulted in the increased use of hybrid quadrupole/orthogonal TOF (QTOF) MS/MS instruments in conjunction with microcolumn LC. Fig. 5 shows a schematic of a QTOF instrument. This LC/MS approach provides a resolution of ca. 0.1 mass... 

The use of MS formats that provide accurate mass capabilities have been recently illustrated for screening combinatorial libraries. The unambiguous confir-mation/identification of combinatorial library components from small quantities of material have been illustrated using a hybrid quadrupole/orthogonal TOE and Fourier transform ion cyclo-... 

It is of interest to mention some detailed mass spectrometric results that were obtained by performing distinct experiments (a) ion-molecule reactions in mixtures of haloben-zenes and ammonia under chemical ionization conditions (b) ion-molecule reactions of mass-selected ionized halobenzenes toward ammonia in a quadrupole collision cell of a hybrid tandem mass spectrometer (c) ion-molecule reactions of phenyl diazonium cations with ammonia in the same quadrupole collision cell and, finally, (d) electrospray ionization of anilines in a hybrid quadrupole-time of flight mass spectrometer (QTof). Characterization of the product ions relies on collisional activation experiments in the low or high kinetic energy regime98. 

This system is very similar to that of a hybrid quadrupole time-of-flight (Q/TOP) instrument but without the initial quadrupole section. 

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