Quantitative profiling

This in fact is what happened. Tandem MS has clearly been shown to be the only technology to screen for disorders of fatty acid oxidation and could also detect many disorders of organic acid metabolism. Tandem MS has the ability to detect both compound classes (amino acids and acylcarnitines) and after demonstrating that both classes could be prepared in the same manner, the MS/MS analysis of blood spots for newborn screening applications was born. 

---

D.K. Han, J. Eng, H. Zhou and R. Aebersold, Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags (ICAT) and mass spectrometry, Nat. Biotech., 19 (2001) 946-951. 

Richardson, D, Ortori CA, Chapman V, Kendall DA, Barrett DA, Quantitative profiling of endocannabinoids and related compounds in rat brain using liquid chromatography-tandem electrospray ionization mass spectrometry, Anal Biochem 360 216-226, 2007. 

Rosello, J., Gelpi, E., Rigaud, M., Durand, J., and Breton, J. C. (1981). Quantitative profiling of the metabolic cascade of arachidonic acid by capillary gas chromatography-mass spectrometry. Biomed. Mass Spectrom. 8, 149-154. 

The use of A/Cs+ to make quantitative profiling easier is illustrated in Fig. 4.39 [128]. Each element in the structure GaAs/AlGaAs/InGaAs/quantum-well (20 X AlGaAs/GaAs)/GaAs is profiled as A/Cs+. 

Meehan KL, Sadar MD. Quantitative profiling of LNCaP prostate cancer cells using isotope-coded affinity tags and mass spectrometry. Proteomics 2004 4(4) 1116-1134. 

Fig. 5 ATR-FTIR measurement of bovine serum albumin [BSA] adsorption on modified PEU surfaces. A ATR-FTIR spectra and B quantitative profiles of BSA adsorption on PEU surfaces modified by MPEO-derived SMAs [81]. Reproduced from [177]...

Cordell, R.L. et al. Quantitative profiling of nucleotides and related phosphate-containing metabolites in cultured mammalian cells by liquid chromatography tandem electrospray mass spectrometry. J. Chromatogr. B. 2008, 871,115-124. 

More recently, a third omics field, lipidomics, was developed. Lipidomics was developed to attempt to provide a system in which to quantitatively profile all of the lipid molecules in a given species or in a tissue within that species (Han, 2011 Welti, 2011). Not surprisingly, a new nomenclature system has been developed to help to define and catalog the various lipids in the lipidome of each species. In this system, tocopherols and tocotrienols are part of the category called prenols (Table 11.1). Currently, the main database for lipidomics information in the USA is the LIPID-MAPS (2011) and its Prenol category contains the four known tocopherols (oc, (3, y, 5), four known tocotrienols (a, [3, y, 5), and a total of 1177 Prenols. Each of the eight individual tocopherols and tocotrienols are sometimes referred to as vitamers. 

Blonder, J., Yu, L.R., Radeva, G., Chan, K.C., Lucas, D.A., Waybright, T.J., Issaq, H.J., Sharom, F.J. and Veenstra, T.D. (2006) Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96. J. Proteome Res. 5, 349-360. 

Ekroos K, Chernushevich IV, Simons K, Shevchenko A. Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer. Anal Chem. 2002 74 941-949. 

Semi-quantitative profiles are used for elements present at low content levels (< 0.3% weight) and/or if the matrix is heterogeneous (the composition of the matrix varies from one analysis point to another). This is the analysis method most commonly used for the study of catalysts. 

The endothelin family consists of three members, ENDO-THELIN-l ENDOTHELIN-2 and ENDOTHELIN-3. All three peptides contain 21 amino acids, but vary in amino acid composition. The three peptides produce vasoconstrictor and pressor responses in various parts of the body. However, the quantitative profiles of the pharmacological activities are considerably different among the three isopeptides. 

Mitchell AE, Morin D, Lakritz J, Jones AD (1997) Quantitative profiling of tissue- and gender-related expression of glutathione S-transferase isoenzymes in the mouse. Biochem J 325 (Pt 1) 207-216. 

G. Cagney, A. Emili, De novo peptide sequencing and quantitative profiling of complex protein mixtures using MCAT, Nat. BiotechnoL, 20 (2002) 163. 

Mass spectrometers are composed of three main components the ionization source, the mass analyzer, and the detector. With the development of different mass analyzers, the choice of mass spectrometer for a specific analysis becomes critical (53), for example, if the analysis is to quantitatively profile all proteins in a sample or quantify a preselected set of protein targets. One or a combination of mass analyzers, such as time of flight (TOF), quadrupole (Q), ion trap (IT), ion cyclotron resonance, and Orbitrap , can be implemented in MS or tandem MS (MS/MS). 

---