Human peripheral blood neutrophils

McColl SR, Hachicha M, Levasseur S, Neote K, Schall TJ. Uncoupling of early signal transduction events from effector function in human peripheral blood neutrophils in response to recombinant macrophage inflammatory proteins-1 alpha and -1 beta. J Immunol 1993 150 4550 1560. 

Jack, R. M., Fearon, D. T. (1988). Selective synthesis of mRNA and proteins by human peripheral blood neutrophils. J. Immunol. 140, 4286-93. 

Bober LA, Waters TA, Pugliese-Sivo CC, Sullivan LM, Narula SK, Grace MJ IL-4 induces neutrophilic maturation of HL-60 cells and activation of human peripheral blood neutrophils. Clin Exp Immunol 1995 99 129-136. 

Unstimulated human peripheral blood neutrophils are known to express relatively high levels of the CXCL8 receptors, CXCRl and CXCR2 [21], Upon stimulation with the corresponding ligands, these cells respond with robust chemotaxis [22], as well as increased adhesion [22,23], superoxide generation... 

Hachicha M, Rathanaswami R Naccache PH, McColl SR Regulation of chemokine gene expression in human peripheral blood neutrophils phagocytosing microbial pathogens. J Immunol 1998 160 449 54. 

Sconocchia G, Cococcetta NY, Campagnano L, Amadori S, lorio B, Boffo V, Ferdinand V, Del Principe I, Adorno D, Casciani CU Subcutaneous administration of interleukin-2 triggers Fcgaimna receptor I expression on human peripheral blood neutrophils in solid and hematologic malignancies. J Iimnunother 2001 24 374-383. 

Larsen E, Kharazmi A, Christensen LP, Christensen SB (2003) An antiinflammatory geilactolipid from rose hip (Rosa canina) that inhibits chemotaxis of human peripheral blood neutrophils in vitro. J Nat Prod 66(7) 994-995... 

Leukocyte-associated IgG egress from plasma is potentially mediated by binding of endogenous IgG or exogenously administered monoclonal antibodies to FCyRI or FCyRIII on circulating neutrophils and monocytes [2] or Fc,RIIa on platelets [24,25], Human peripheral blood monocytes also bear MHC class I-related FcRn that can transport IgG from plasma to tissue fluids, dendritic cells, or intestinal macrophages [26],... 

Harvath, L., Falk, W., and Leonard, E. J. (1980) Rapid quantitation of neutrophil chemotaxis use of a polyvinylpyrrolidone-free polycarbonate membrane in a multi well assembly. J Immunol Methods 37,39-45 9 Van Epps, D. E., and Chennoweth, D. E. (1984) Analysis of the binding of fluorescent C5a and C3a to human peripheral blood leukocytes. J. Immunol 132, 2862-2867. 

Kita, H., Ohnishi, T., Okubo, Y., WeUer, D., Abrams, J. S. and Gleich, G.J. (1991). Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils. J. Exp. Med. 174, 745-748. 

GDIS (LeuMI) C3D-1 3-fucosyl-N- acetyllactosamine Purified neutrophils from normal human peripheral blood Ventana NA HIER... 

Hurttia, H. Leino, L. Subcellular localization of diacylglycerol kinase activity in stimulated and unstimulated human peripheral blood lymphocytes and neutrophils. Biochem. Mol. Biol. Int., 40, 579-585 (1996)... 

Exposure to air polluted with particles less than 2.5 pm (2500 nm) in size was associated with epidemiologically adverse cardiopulmonary health consequences in humans. In this study, six healthy volunteers were exposed to fine and ultrafine but nontoxic magnesium oxide (MgO) particles (70a). Exposiue levels were mean + standard deviation for exposure was 4,138 + 2163 minutes x mg/m. Ninety-eight percent of the particles were fine (<2.5 pm 2500 nm) and 28% were ultrafine (< 100 nm). No differences were noted from controls, as indicated by bronchoalveolar lavage (BAL) inflammatory cell concentrations, interleukin concentrations (ILl, IL6, IL8), tumor necrosis factor, pulmonary function, or peripheral blood neutrophil concentrations (70a). Thus, volunteers who inhaled nontoxic MgO at high levels over considerable time periods showed no indications of inflammation in the lung as indicated by BAL washings. 

IL-8 has chemotactic activity not oiily for neutrophils but also basophils (13) and IL-3-or granulocyte-macrophage colony-stimulated factor (GM-CSF)-primed eosinophils (14). IL-8 also induces chemotaxis against CD4+ and CD8 human peripheral blood T lymphocytes (15). 

Multiple factors associated with copper deficiency are responsible for the increased rate of infection seen. Most copper-deficient patients are malnourished and suffer from impaired weight gain. The immune system requires copper to perform several functions. Recent research showed that interleukin 2 is reduced in copper deficiency and is probably the mechanism by which T-cell proliferation is reduced. These results were extended to show that even in marginal deficiency, when common indexes of copper are not affected by the diet, the proliferative response and interleukin concentrations are reduced. The number of neutrophils in human peripheral blood is reduced in cases of severe copper deficiency. Not only are they reduced in number, but their ability to generate superoxide anion and kill ingested microorganisms is also reduced in both overt and marginal copper deficiency. This mechanism is not yet understood. 

Figure 4.12 Identification of different cell types in a mixed population of human peripheral blood leukocytes (a) neutrophils identified with [Eu(L5)]-anti HNL, (b) T-lympbocytes illuminated by [Tb(L6)r-CD3, and (c) eosinophils imaged by [Pt(L7a)f. Reproduced with permission from [46]. Copyright 2003, John Wiley...

Although controversial, findings as to how chronically administered morphine modulates neutrophil chemotaxis and function, a growing consensus believes that morphine is suppressive in the recruitment and functional aspects of these cells during an innate immune response. When peripheral human blood neutrophils were pretreated with exogenous opioids, lL-8-induced chemotaxis was inhibited (Grimm et al. 1998). Conversely, Simpkins et al. reported an increase in neutrophil chemotaxis... 

Neutrophils are the most abundant leukocytes in humans, comprising about two thirds of peripheral blood leukocytes. Upon tissue injury, they rapidly infiltrate injury sites and play an important role in innate immune responses. In addition, they also contribute to the development of adaptive immune responses by producing an array of cytokines and chemokines. Tissue infiltration of neutrophils is initiated by signals generated by the interaction between chemoattractants produced at sites of injury and their corresponding cell surface receptors. Classical chemoattractants, such as C5a, N-formyl-methionyl-leucyl-... 

Thus, oxygen radical production by leukocytes can be responsible for cancer development. However, the levels of leukocyte oxygen radical generation depend on the type of cancer. For example, PMNs and monocytes from peripheral blood of patients with lung cancer produced a diminished amount of superoxide [169], Timoshenko et al. [170] observed the reduction of superoxide production in bronchial carcinoma patients after the incubation of neutrophils with concanavalin A or human lectin, while neutrophils from breast cancer patients exhibited no change in their activity. Chemotherapy of lung and colorectal carcinoma patients also reduced neutrophil superoxide production. Human ALL and AML cells produced, as a rule, the diminished amounts of superoxide in response to PMA or FMLP [171], On the other hand total SOD activity was enhanced in AML cells but diminished in ALL cells, while MnSOD in AML cells was very low. It has been proposed that decreased superoxide production may be responsible for susceptibility to infections in cancer patients. 

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