Human hepatocellular carcinoma

The amino acid sequence of the human erythrocyte glucose transporter was deduced from the nucleotide sequence of a cDNA clone in 1985 [106]. Polyclonal antibodies raised against the protein were used to screen a Xgtl I cDNA library prepared from the human hepatocellular carcinoma cell line HepG2. (Like many other transformed... 

Seco-3,4-taraxerone and seco-3,4-friedelin 2 abrogated the survival of human hepatocellular carcinoma (Hep-G2) and human epidermoid carcinoma (A-431) cell lines cultured in vitro with IC50 values of 11.7 and 38.2 mM, and inhibited the enzymatic activity of topoisomerase at dose of 7 pM (35). 

Seow TK et al. Two-dimensional electrophoresis map of the human hepatocellular carcinoma cell line, HCC-M, and identification of the separated proteins by... 

Kullak-Ublick GA, Glasa J, Boker C, Oswald M, Grutzner U, Hagenbuch B et al. Chlorambucil-taurocholate is transported by bile acid carriers expressed in human hepatocellular carcinomas. Gastroenterology 1997 113 (4) 1295—1305. 

Breuhahn K, Longerich T, Schirmacher P (2006). Dysregulation of growth factor signaling in human hepatocellular carcinoma. Oncogene 25 3787-3800. 

Lee JS, Thorgeirsson SS (2005). Genetic profiling of human hepatocellular carcinoma. Semin Liver Dis 25 125-132. 

Thorgeirsson SS, Grisham JW (2002). Molecular pathogenesis of human hepatocellular carcinoma. Nat Genet 31 339-346. 

Tsou AP, Wu KM, Tsen TY, Chi CW, Chiu JH, Lui WY et al (1998). Parallel hybridization analysis of multiple protein kinase genes identification of gene expression patterns characteristic of human hepatocellular carcinoma. Genomics 50 331 340. 

Gu, J. X. Elevated activity of N-acetylglucosaminyltransferase V in human hepatocellular carcinoma. 

Hou et also demonstrated that dihydroartemisinin (39), artemether (40) and artesunate (41) (Fig. 4) were able to inhibit the growth of four human hepatocellular carcinoma cells (HepG2, p53 wild-type Hep3B, p53 null Huh-7, p53 mutant and BEL-7404, p53 mutant). The IC50 values averaged 10.8 /tmol/L (1), 10.6 /tmol/L (39), 21.0 /tmol/L (41), and... 

HepG2 p53 wild-type human hepatocellular carcinoma... 

Feng, B. Effects of extracts from Seeds of Cerbera Manghas on Cell proliferation, cell Cycle progression and apoptosis of human hepatocellular carcinoma HepG2 Cells... 

Kuramitsu Y, Nakamura K. Current progress in proteomic study of hepatitis C virus-related human hepatocellular carcinoma. Expert Rev Proteomics 2005 2 589-601. 

Fujii K, Kondo T, Yokoo H et al. Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye. Proteomics 2005 5 1411-1422. 

Zeindl-Eberhart E, Haraida S, Liebmann S et al. Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas. Hepatology 2004 39 540-549. 

Yu H, Pan C, Zhao S, Wang Z, Zhang H, Wu W. 2008. Resveratrol inhibits tumor necrosis factor-alpha-mediated matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. Biomed Pharmacother. 62 366-372. 

Fibrinogen is assembled in the rough endoplasmic reticulum of the liver. In human hepatocellular carcinoma cells, the newly synthesized Bj3 chains are secreted and used more rapidly than the Aa and 7 chains, so that a large intracellular pool of free Aa and 7 chains exists (Yu et al, 1984). In contrast, the Aa chains are the limiting factor in cultured rat or chicken hepatocytes (Hirose et al., 1988 Plant and Grieninger, 1986). Stimulation by serum results in a 20-fold increase in secretion of fibrinogen and comparable levels of all three chains (Baumhueter et al, 1989). 

Wolschek MF, Thallinger C, Kursa M et al (2002) Specific systemic nonviral gene delivery to human hepatocellular carcinoma xenografts in SCID mice. Hepatology 36 1106-1114... 

HEPES Ar-(2-hydroxyethyl)piperazine-A,-(2-ethanesulfonic acid) HepG2 human hepatocellular carcinoma cells HFE a gene for hemochromatosis... 

Dubuisson L, Monvoisin A, Nielsen BS, Le BB, Bioulac-Sage P, Rosenbaum J. Expression and cellular localization of the urokinase-type plasminogen activator and its receptor in human hepatocellular carcinoma. J Pathol 2000 190(2) 190-195. 

Liang CR, Leow CK, Neo JC, Tan GS, Lo SL, Lim JW, et al. Proteome analysis of human hepatocellular carcinoma tissues by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 2005 5(8) 2258-2271. 

Sugeno H, Takebayashi Y, Higashimoto M, Ogura Y, Shibukawa G, Kanzaki A et al. Expression of copper-transporting P-type adenosine triphosphatase (ATP7B) in human hepatocellular carcinoma. Anticancer Res 2004 24 1045-8. 

Buchner RR, Hugh TE, Ember JA, Morgan EL (1995) Expression of functional receptors for human C5a anaphylatoxin (CD88) on the human hepatocellular carcinoma cell line HepG2. Stimulation of acute-phase protein-specific mRNA and protein synthesis by human C5a anaphylatoxin. J Immunol 155 308-315. 

Butein is a simple chalcone formed by the union of a phloroglucinol ring with a catechol one through a propenone chain. It has been characterised as a PTK specific inhibitor, because it reduced the extent of the EGF-induced phosphorylation of the EGF receptor localised in human hepatocellular carcinoma cells, and the activity of the soluble EGF receptor and of p60 ". In contrast with other flavonoids, butein was ineffective against serine/threonine protein kinases [55]. 

Dragovic, T. et al. (1994) Increased expression of neprilysin (neutral endopeptidase 24.11) in rat and human hepatocellular carcinomas. Lab. Invest., 70.107-113. 

Kanazawa Y, Ohkawa K, Ueda K, Mita E, Takehara T, et al. 1996. Hammerhead ribozyme-mediated inhibition of telomerase activity in extracts of human hepatocellular carcinoma cells. Biochem. Bio-phys. Res. Commun. 225 570-76... 

Knowles and Milner, 2000). Furthermore, these compounds are thought to be involved in the inhibition of certain cytochrome P-450 enzyme-dependent bioactivations of procarcinogens and protoxicants (Brady et al., 1991), as well as to increase levels of glutathione-S-transferase (GST), an enzyme of particular importance in the detoxification of xenobiotics in the body (Wilce and Parker, 1994). Most recently, the ability of various plants and plant extracts to influence apoptosis, or programmed cell death, in cancerous cells in an attempt to arrest their proliferation, has been the topic of much research. Allicin has been shown to induce apoptosis in a variety of cell lines, including human hepatocellular carcinoma cells (KIM-1) and human lymphoid leukemia (MOLT-4B) cells (Thatte et al., 2000). 

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