Two-dimensional difference gel

Kondo T, Hirohashi S. (2006) Application of highly sensitive fluorescent dyes (CyDye DICE Fluor saturation dyes) to laser micro-dissection and two-dimensional difference gel electrophoresis (2D-DICE) for cancer proteomics. NatProtoc 1,2940-56. 

Shaw J, Rowhnson R, Nickson J, et al. (2003) Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes. Proteomics 3, 1181-95. 

Fujii K, Kondo T, Yokoo H et al. Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye. Proteomics 2005 5 1411-1422. 

DE, two-dimensional gel electrophoresis WB, Western blot MS, mass spectrometry LC, liquid chromatography 2DE DICE, two-dimensional difference gel electrophoresis, SRM, selected-reaction monitoring MRM, multiple reaction monitoring AQUA, absolute protein quantitation SMIM, selected MS/MS ion monitoring. ... 

Hobson, D.J., Rupa, P., Diaz, G.J., Zhang, H., Yang, M., Mine, Y., Turner, P.V., Kirby, G.M. 2007. Proteomic analysis of ovomucoid hypersensitivity in mice by two-dimensional difference gel electrophoresis (2D-DIGE). Food Chem Toxicol 45 2372-2380. 

Liang CR, Leow CK, Neo JC, Tan GS, Lo SL, Lim JW, et al. Proteome analysis of human hepatocellular carcinoma tissues by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 2005 5(8) 2258-2271. 

Alban, A., David, S. O., Bjorkesten, L, Andeesson, C., Sloge, E., Lewis, S., Currie, I. (2003). A novel experimental design for comparative two-dimensional gel analysis two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3, 36-44. 

Determining a significant change in protein expression with DeCyder during a pairwise comparison using two-dimensional difference gel electrophoresis. Proteomics 4, 1421-1432. 

Sakai, J., Ishikawa, H., Kojima, S., Satoh, H., Yamamoto, S., and Kanaoka,M. (2003) Proteomic analysis of rat heart in ischemia and ischemia-reperfusion using fluorescence two-dimensional difference gel electrophoresis. Proteomics 3,1318-24. 

Van den Bergh, G., Clerens, S., Vandesande, F., and Arckens, L. (2003) Reversed-phase high-performance liquid chromatography prefractionation prior to two-dimensional difference gel electrophoresis and mass spectrometry identifies new differentially expressed proteins between striate cortex of kitten and adult cat. Electrophoresis 24, 1471-81. 

Yan, J. X., A. T. Devenish, R. Wait, T. Stone, S. Lewis, and S. Fowler. 2002a, Fluorescence two-dimensional difference gel electrophoresis and mass spectrometry based proteomic analysis of Escherichia coli./ roteo/Mici. 2 1682-1698. 

Fig. 17.9. Workflow for a standard two-dimensional difference gel electrophoresis (DICE) experiment. After being labeling separately with different dyes, individual samples can be compared on a single gel. Thereby, experimental variation is reduced and spot matching is improved. Using an internal standard, i.e., a pool of all the samples within an experiment.

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