Human epithelial carcinoma

Table I. The Effect of Various Compounds Implicated in the 2-Stage Theory of Carcinogenesis on CMP-NeuAc lactosylceramide Sialyltransferase Activity in Human Epithelial Carcinoma with (KB) Cells...

Human epithelial carcinoma None Retinoic acid Moskal et al. (1980)... 

Thati, B. Noble, A. Creaven, B. S. Walsh, M. McCann, M. Kavanagh, K. Devereux, M. Egan, D. A. In vitro antitumor and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma ceU hnes. Cancer Lett. 2007,248,321-331. 

Hiribarren, A., Heyman, M., L Helgouac h, A. and Desjeux, J.F. (1993) Effect of cytokines on the epithelial function of the human colon carcinoma cell line HT29 cll9A. Gut 34, 616-620. 

The enthusiasm for using Caco-2 cells and other epithelial cell cultures in studies of drug transport processes has been explained by the ease with which new information can be derived from these fairly simple in vitro models [7]. For instance, drug transport studies in Caco-2 cells grown on permeable supports are easy to perform under controlled conditions. This makes it possible to extract information about specific transport processes that would be difficult to obtain in more complex models such as those based on whole tissues from experimental animals. Much of our knowledge about active and passive transport mechanisms in epithelia has therefore been obtained from Caco-2 cells and other epithelial cell cultures [10-15]. This has been possible since Caco-2 cells are unusually well differentiated. In many respects they are therefore functionally similar to the human small intestinal enterocyte, despite the fact that they originate from a human colorectal carcinoma [16, 17]. 

Borchardt. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability, Gastroenterology 1989, 96, 736—749... 

E., Brattain, M. G., Zweibaum, A., Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells a survey of twenty cell lines, Cancer Res. 1988, 48, 1936-1942. 

Grasset, E., Pinto, M., Dussaulx, E., Z WEI BAUM, A., Desjeux, J.-F., Epithelial properties of human colonic carcinoma cell line Caco-2 electrical parameters, Am. J. Physiol. 1984, 247, C260-C267. 

A549 Human lung carcinoma epithelial-like... 

Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G (1976) A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer 17(1 ) 62—70... 

In contrast to P-gp and the MRP proteins, the breast cancer resistance protein (BCRP) contains six transmembrane domains and only one ATP-binding domain. It was first cloned from the breast cancer cell line MCF-7 selected in doxombicin, in the presence of the P-gp inhibitor verapamil. It is found in many human tissues, such as the placenta, small intestine, colon, and liver [133], It is localized to the apical membrane of epithelial cells of the small intestine and colon and to the bile canalicular membrane in the liver and is involved in reducing intestinal uptake, increasing hepatobiliary excretion, etc., leading to diminished oral bioavailability. cDNA sequences identical to BCRP and named MXR and ABCP, respectively, were independently isolated from human colon carcinoma cells and human placenta [134], BCRP requires... 

The Caco-2 cells are derived from human colorectal carcinoma. When these cells are cultured on semipermeable membranes, they grow into epithelial cells that are very similar to intestinal epithelial cells. The permeability of drugs across these Caco-2 cells provides model tools for the study of drug absorption. 

Wilkinson, M. J. S., Howell, A., Harris, M., Taylor-Papadimitriou, J., Swindell, R. and Sellwood, R. A. 1984. The prognostic significance of two epithelial membrane antigens expressed by human mammary carcinomas. Int. J. Cancer 33, 299-304. 

Cadherins. Cadherins are a group of glycoproteins that facilitate Ca2+-dependent cell-cell adhesive interactions.20 When cadherin function is disrupted, the release of a tumor cell can result. It was shown that the aggressive metastasis of undifferentiated epithelial carcinoma cells that had lost cell-cell adhesion could be stopped by transfection with E-cad-herin cDNA.21 Therefore, it was suggested that E-cadherin suppresses metastasis,22 which was further supported by more recent studies that showed the loss of adhesion of human gastric, prostatic, and lung cancer cells was due to the gene mutation of a protein associated with the proper function of cadherin.23... 

Kepivance has been shown to enhance the growth of human epithelial tumor cell lines in vitro (15x MRHD) and to increase the rate of tumor cell line growth in a human carcinoma xenograft model (25-67x MRHD)... 

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