High-pressure liquid chromatography comparison

Chase, G.W. Jr., Akoh, C.C., and Eitenimiller, R.R. 1994. Analysis of tocopherols in vegetable oils by high-pressure liquid chromatography Comparison of fluorescence and evaporative lightscattering detection. J. Am. Oil. Chem. Soc. 71 877-880. 

Koup, J.R. and Brodsky, B., Comparison of homogeneous enzyme immunoassay and high pressure liquid chromatography for the determination of theophylline concentration in serum, Am. Rev. Respir. Dis., 117,1135,1978. 

CS048 Wheals, B. B. and R. N. Smith. Comparative cannabis analysis. A comparison of high-pressure liquid chromatography with other chromatographic techniques. J Chromatogr 1975 105 396-400. 

Internal reflection spectroscopy is widely applied for on-line process control. In this type of application, the chemical reactor is equipped with an internal reflection probe or an IRE. The goal of this type of application is the quantification of reactant and/or product concentrations to provide real-time information about the conversion within the reactor. In comparison with other analytical methods such as gas chromatography, high-pressure liquid chromatography, mass spectrometry, and NMR spectroscopy, ATR spectroscopy is considerably faster and does not require withdrawal of sample, which can be detrimental for monitoring of labile compounds and for some other applications. 

JW DeVries, CM Koski, DC Egberg, PA Larson. Comparison between a spectrophotometric and a high-pressure liquid chromatography method for determining tryptophan in food products. J Agric Food Chem 28 896-898, 1980. 

The GC is often connected to a mass spectrometer. Mass spectrometry (MS) breaks samples apart and separates the ionized fragments by mass and charge. Vast libraries of comparison fragments make computer-aided identification of materials possible even when the sample is very small. Most forensic laboratories have access to a combined gas chromatograph/mass spectrometer (GC/MS). High pressure liquid chromatography (HPLC) separates many types of drugs and may also be combined with MS. 

Mirrlees MS, Moulton SJ, Murphy CT et al. (1976) Direct measurement of octanol-water partition coefficients by high-pressure liquid chromatography. J Med Chem 19 615-619 Pagliara A, Khamis E, Thrinh A et al. (1995) Structural properties governing retention mechanisms on RP-HPLC stationary phases used for lipophilicity measurements. J Liquid Chromatography 18 1721-1745 Slater B, McCormack A, Avdeef A et al. (1994) pH-Metric log P. 4. Comparison of Partition Coefficients Determined by Shake-Flask, HPLC and Potentiometric Methods. J Phar-maceut Sci 83 1280-1283... 

Buchardt O, Jensen RB, Hansen HF, Nielsen PE, Andersen D, Chinoin I (1986) Thermal chemistry of podophyllotoxin in ethanol and a comparison of the cytostatic activity of the thermolysis products J Pharm Sci 75 1076-1080 Charalambous G, Bruckner KJ, Hardwick WA, Linneback A (1973) Determination of beer flavor compounds by high pressure liquid chromatography Tech Quart, Master Brew Assoc Am 10(2) 74-78... 

A comparison of the results obtained by high pressure liquid chromatography (HPLC) with results obtained by other techniques such as inductively coupled plasma emission spectroscopy (ICPES) showed good agreement and HPLC procedures are found to be economical in terms of analysis time. 

High Pressure Liquid Chromatography. In blood, plasma or urine diazepam and metabolites, sensitivity 50 ng/ml in blood or plasma, 200 ng/ml in urine, UV detection—S. Cotier et al, J. Chromat., 1981, 222 Biomed. Appl., 11, 95-106. In plasma diazepam and desmethyldiazepam, comparison with a gas... 

High Pressure Liquid Chromatography. In plasma UV detection, comparison with a GC method—R. N. Gupta and F. Eng, J. High Resolut. Chromat. Chromat. Commun., 1980, i, 419-420. 

High Pressure Liquid Chromatography. In plasma sulphapyridine and metabolites, detection limit 1 pg/ml, UV detection—C. Fischer and U. Klotz, J. Chromat., 1978,146 Biomed. AppL, 3, 157-162. In plasma, saliva or urine comparison with GC, detection limits 50 ng for HPLC (fluorescence detection), 10 ng for GC (ECD)—M. E. Sharp et al., Can. J. pharm. Sci., 1980,15, 35-38. 

Preferably, high pressure liquid chromatography (hplc) is used to separate the active pre- and cis-isomers of vitamin D3 from other isomers and allows theic analysis by comparison with the chromatograph of a sample of pure reference j-vitamin D3, which is equilibrated to a mixture of pre- and cis-isomers (82,84,85). This method is more sensitive and provides information on isomer distribution as well as the active pre- and cis-isomer content of a vitamin D sample. It is applicable to most forms of vitamin D, including the more dilute formulations, ie, multivitamin preparations containing at least 1 lU/g (AOAC Methods 979.24 980.26 981.17 982.29 985.27) (82). The practical problem of isolation of the vitamin material from interfering and extraneous components is the limiting factor in the assay of low level formulations. 

Joos, B. Ledergerber, B. Flepp, M. Bettex, J.-D. Luthy, R. Siegenthaler, W. Comparison of high-pressure liquid chromatography and bioassay for determination of ciprofloxacin in serum and urine. Antimicrob.Agents Chemother., 1985,27, 353—356... 

Each model was applied to sugar attenuation data taken from multiple brewing fermentations (assessed using high-pressure liquid chromatography). Three techniques Akaike s (corrected) Information Criterion, comparison of the coefficients of determination (r ) and absolute residual sum of squares (RSS) were used to compare the fit of each model. Ideally, the data would adhere to a simplistic, theoretically derived formula such as a low parameter symmetric model. Unfortunately, the variability in both shape and lag time for each individual sugar necessitated a more flexible... 

Christie (1990), in an overview of TLC, asks the question Has thin-layer chromatography had its day . Comparison of this simple technique with the newer and more sophisticated high pressure liquid chromatography (HPLC) makes TLC look obsolete. However, as also agreed by the author, traditional TLC is still used and is likely to be around for many years to come. Between the years 1990 and 1996 TLC has found application in several fields, and publications relate to food (18%), medical, clinical and biological applications (26%), pharmaceuticals (25%), environmental studies (11%), detection (14%) and chemical synthesis monitoring (6%) (Weins and Hauck, 1995). It is estimated that there were between 500-900 articles per... 

We have evidenced the difference between the reactivity of the MD and CE by measuring the reaction rate of I with CE and MD separately. We have shown the existence of a supplementary synergetic effect due to which, in the case of the reaction between I with the mixture MD-CE, the reactivity of the glycol increases in comparison to that of MD [44—46,89]. With this aim, high pressure liquid chromatography (HPLC) measurements where employed to monitor the evolution of every molecular species which can appear in the reaction, on the model reaction which employs a monoisocyanate instead of the diisocyanate [89]. 

Fig. 19. Schematic comparison between SFA (segmented-flow analysis, A), FIA (flow-injection analysis, B), and HPLC (high-pressure liquid chromatography, C).

Evaluation and Comparison of Enzyme-immunoassay, Gas-Liquid Chromatography and High-Pressure Liquid Chromatography for the Monitoring of Antiepileptic Drugs in Serum Enzyme Labelled Immunoassay Horm. Drugs, Proc. Int. Symp. 1978 405-418 CA 91 204051f... 

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