High pressure liquid chromatography method

High-pressure liquid chromatography method development of pharmaceuticals is an iterative process required to support successive phases of pharmaceutical development and clinical studies. This chapter details the approach currently in use in our laboratories, from receipt of a new chemical entity to post transfer support. 

JW DeVries, CM Koski, DC Egberg, PA Larson. Comparison between a spectrophotometric and a high-pressure liquid chromatography method for determining tryptophan in food products. J Agric Food Chem 28 896-898, 1980. 

The methods of RNA isolation depends on the tissue and type of RNA to be extracted. Procedures to isolate total cellular RNA include chemical extractions and centrifugation. mRNA is isolated from total RNA using affinity chromatography or magnetic beads, while high-pressure liquid chromatography methods are used for small RNA molecules. Phenol extraction was one of the first techniques to isolate RNA successfully from many sources... 

D29. Dunn, P. J., Cole, R. A., and Soeldner, J. S., Further development and automation ofa high pressure liquid chromatography method for the determination of glycosylated hemoglobins. Metabolism 28, TTl-Tl A (1979). 

Hogarth, L. A. Rabello, C. M. A. Hall, A. G. Measurement of reduced glutathione using high-pressure liquid chromatography. Methods Mol. Med. 1999, 28, 91-94. 

Castiglioni S, Bagnati R, Calamari D, Fanelli R, Zuccato E (2005) A multiresidue analytical method using solid-phase extraction and high-pressure liquid chromatography tandem mass spectrometry to measure pharmaceuticals of different therapeutic classes in urban waste-waters. J Chromatogr A 1092 206-215... 

Improved high-pressure liquid chromatography (HPLC) methods have been developed for the analysis of quaternary salt type corrosion inhibitors in brine waters [400]. However, these methods are not suitable for imidazolines and amido-amines. A method based on fluorescence detection has been described for the quantitative analysis of the imidazoline- and amido-amine-type corrosion inhibitors in both oil field water and crude oil samples by HPLC [1174]. 

For many decades, the standard technique for measuring carotenoids has been high-pressure liquid chromatography (HPLC). This time consuming and expensive chemical method works well for the measurement of carotenoids in serum, but it is difficult to perform in human tissue since it requires biopsies of relatively large tissue volumes. Additionally, serum antioxidant measurements are more indicative of short-term dietary intakes of antioxidants rather than steady-state accumulations in body tissues exposed to external oxidative stress factors such as smoking and UV-light exposure. 

The most commonly used method for the identification of carotenoids is high-pressure liquid chromatography (HPFC) combined with the UV-Vis absorption detection. The introduction of diode array detection enabled parallel collection of pigment spectra, which greatly aids the quantification and localization of unknown compounds. Coupling HPFC with the mass-spectrometer significantly... 

In the case of heterogeneous polymers the experimental methods need to be refined. In order to analyze those polymers it is necessary to determine a set of functions / (M), which describe the distribution for each kind of heterogeneity i This could be the mass distributions of the blocks in a diblock copolymer. The standard SEC methods fail here and one needs to refine the method, e.g., by performing liquid chromatography at the critical point of adsorption [59] or combine SEC with methods, which are, for instance, sensitive to the chemical structure, e.g., high-pressure liquid chromatography (HPLC), infrared (IR), or nuclear magnetic resonance spectroscopy (NMR) [57],... 

High-pressure gas separation, hollow-fiber membrane modules for, 15 823 High pressure liquid chromatography (hplc), 9 234 21 275 in herbicide analysis, 13 312 polymer analysis using, 19 566 High-pressure methanol, production process, 16 300-301 High pressure methods, specialized, 13 430-431... 

High pressure liquid chromatography is the standard method used to quantify the API and related impurities. It is often used to measure solubility. 

Anion-exchange high-pressure liquid chromatography provides an excellent method for the determination of adenosine phosphates.226 Nucleotide phosphates have been concentrated by ion-exchange chromatography.227... 

Isolation of individual amino acids started about 1820 by 1904 all of the naturally occurring amino acids in proteins had been isolated except methionine (Mueller, 1922) and threonine (Rose, 1937). One of the earliest methods for the separation of amino acids was through the differential volatility of their methyl or ethyl esters (Emil Fischer, 1901). This approach led to the discovery of valine, proline, and hydroxyproline. [In the 1970s Fischer s method was modified for microanalysis of proteins, separating the amino acid esters by gas phase chromatography. Separation is now usually performed by hplc (high pressure liquid chromatography).]... 

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