Glass fiber membranes

Filament material Aluminum borosilicate glass fibers Membrane material Pd/Ag (23 wt.-%)... 

Lim TK, Imai S, Matsunaga T (2002) Miniaturized amperometric flow immunoassay system using a glass fiber membrane modified with anion. Biotechnol Bioeng 77 758-763... 

In preparation for sequencing, the peptide (normally 100 pmol, but the optimal quantity depends on the sequencer being utilized) dissolved in acetonitrile or methanol is spotted onto a membrane [for example, a coated glass-fiber membrane (Biobrene ABI) or a polyvinylidene difluoride (PVDF) membrane such as Immobilon (Millipore) or Problott (ABI)] the sample is air dried and then the membrane is loaded directly into the sequence cartridge. If severe problems with washout are experienced, techniques for covalent coupling of a peptide to the membrane can be employed, provided that specific residues (e.g., an amino acid with a free amine on its side chain) are present in the sequence. However, a drawback of this approach is that amino acids that are covalently coupled will not produce a signal in the subsequent HPLC analysis. 

Airborne particles commonly are collected from air on filter media such as organic membranes, paper (cellulose fiber), or glass fibers. Membrane filters are fragile and... 

Membranes from glass fiber coated with polybrene (GF/C of Whatman) are suitable for amino acid sequencing because the membranes are inert against the chemicals used for the sequencing. Although the protein binding capacity of the coated glass fiber membranes (10 to 30 pg/cm ) is comparable to that of nitrocellulose, most sequencers prefer PVDF membranes. 

Unifilter-GF / Cfilter plates, glass fiber membrane filter 96-well plates with a 1.2 pM pore size (PerkinElmer) to collect radioHgand-bound membranes. 

Cut the protein strips into small pieces with the dimensions 2x2 mm and place them into the standard sequencing reaction cartridge supported by a polybrene-coated glass fiber membrane. 

Separation based on the isoelectric point difference can also be obtained on glass fiber membrane modified with anion [87] or on a multichanneled matrix column coated with cation-exchange resin [30]. This electrochemical flow immunoassay system was also used for the assay of HCG, the tracer being a monoclonal antibody labeled with ferrocene and prepared as shown in Scheme 8.6 [31,87]. A linear relationship was observed between 10 and 2000 ml mL of HCG, indicating that this immunoassay is suitable for diagnosis of pregnancy and/or gonadal tumors in... 

After detection, the peptide spots on the cellulose or silica gel thin layers are carefully scraped and are transferred to Pasteur pipets (150 mm long) that have been tightly plugged with one-fourth of a glass fiber membrane filter (20-mm diameter, Sartorious SM 13400) and prewashed with 2 ml of 6N HCl 200 pi of 6N HCl containing 0.02% 2-mercaptoethanol is added to each of the Pasteur pipets and the peptide is extracted at room temperature for 15 min. The HCl is then forced through the filter with nitrogen (1 atm). 

The flow along the membranes also improves the mass transport there, and the separators between the membranes are constmcted to provide good flow distribution and mixing on the membrane surfaces. Membrane sizes are often about 0.5 x 1 m, spaced about 1 mm apart. Many types of polymers are used to manufacture these ion-exchange-selective membranes, which are often reiaforced by strong fabrics made of other polymers or glass fibers. 

Membranes are applied directly to the metal or concrete surface to protect from corrosion by any liquor that penetrates the brick lining through pores and cracks. Membranes consist of sheet material bonded to the metal or concrete, e.g., flexible PVC sheet, or it may be formed in situ (e.g., polyester resin reinforced with glass fiber, or synthetic rubber sheet, lead, polyisobutylene, polyethylene and asphalt). 

Cross-flow ultrafdtration equipment.—The device used is shown in Figure 1. It included a glass reactor (R) with temperature, pH and stirring control, a Minitan pump (P) (Millipore, Bedford, USA), a Harp hollow fiber membrane cartridge (M) (Romicon-Supelco, Bellefonte, USA) with a cut-off of 2000 daltons, and a permeate exit (f) for fraction collection. The retentate (r) was returned to the reactor. 

Wang, S.S. Ricauite, G.A. and Peroutka, S.J. [ H]3,4-methylenedioxy-methamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper. Eur J Pharmacol 138 439-443, 1977. 

While nuclepore polycarbonate membranes (0.4 ]im) were preferred as backup filters when sulfate was to be measured, glass-fiber filters had to be used in the HVI measurements. 

An optical-fiber CL sensor is reported for trichlorethylene assay [87], The sensor consists of a glass fiber bundle and a transducer consisting of three components (i) a gas-permeable membrane to separate trichlorethylene from water, (ii) H2S04-NaN03 mixture as oxidizing agent, and (iii) a luminol solution. The assay of trichloroethylene can be done in the 0.05-0.6- J,g/mL concentration range with a detection limit of 0.03 J.g/mL. 

All water samples were collected in amber-polyethylene terephtalate (PET) bottles and were kept at 4°C during shipment. Upon reception in the laboratory, samples were vacuum filtered through 1 pm glass fiber filters, followed by 0.45 pm nylon membrane filters, and were stored in the dark at —20°C until analysis. 

Hollow fiber membrane(s), 70 766 76 1-31 additional types of, 76 24 advantages of, 76 3 categories of, 76 2-3 in desalination, 76 22 development of, 76 1 extractors, 70 787 fiber treatment for, 76 12-18 future prospects for, 76 26-28 glass and inorganic, 76 23-24 handling and unit assembly of, 76 15-18 interpenetrated wall matrix in, 76 15 low pressure, 76 24-26 macrovoids in, 76 12 materials associated with, 76 18-24 melt spinning of, 76 9-10... 

Inorganic glass systems, 12 568t Inorganic halogen compounds, hydrogen chloride reaction with, 13 820 Inorganic hollow-fiber membranes, 16 23-24... 

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