Anion gene transfer

Freulon, I., Monsigny, M., Midoux, P. and Mayer, R. (2000) Spacer length dependence on the efficiency of dimeric anionic peptides in gene transfer by glycosylated polylysine/ plasmid complexes. Biosci. Rep., 20, 383-398. 

Lee R, Huang L (1996) Folate-targeted, anionic Hposome-entrapped polylysine-con-densed DNA for tumor cell-specific gene transfer. J Biol Chem 271 8481-8487... 

Le Doux, J.M. et al., Complexation of retrovirus with cationic and anionic polymers increases the efficiency of gene transfer. Hum. Gene Ther., 12,1611,2001. 

Liposomes are a heterogeneous class of compounds that can be synthesized so that they possess a wide variety of physical and chemical properties. They can consist of sheets and long cylinders of reasonably circular vesicles such structures are the result of combining phospholipids with a fatty acid tail and hydrophilic head groups. There are two classes of these compounds that have been extensively studied as vectors for gene transfer anionic liposomes and cationic liposomes. 

With the optimized lipid composition (opDC ePC DOPE eSph Choi DC-Chol = 5 5 5 12 3), the HVJ-cationic liposomes showed 100 to 800 times greater transfection efficiency in vitro compared with the conventional HVJ-PS liposomes. The presence of serum (10% FCS) in the transfection mixture did not decrease luciferase activity significantly. Even 70% FCS reduced the activity by less than 40%. LacZ gene expression showed that transfection efficiency of BHK-21 cells by optimized HVJ-cationic liposomes (opDC) and by conventional HVJ-cationic liposomes (DC) was 90-100% and 50-60%, respectively. With conventional HVJ-anionic liposomes (PS), LacZ expression was found in only 1-3% of the cells. The optimized HVJ-cationic liposomes were also much more effective for the transfer of FITC-labeled ODNs to cultured cells [16]. 

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