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Gene transfer using lipoplexes

Vesicle-Nucleic Acid Interactions Gene Transfer Using Lipoplexes... [Pg.438]

Reports of CNS gene transfer using cationic liposomes began to appear in the literature from the early 1990s. DNA-liposome complexes (lipoplexes)... [Pg.465]

Cationic liposomes composed of 3(3- [ N- (N N-dimethylaminoethane (carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidylethanolamine (DOPE) (DC-Chol/DOPE liposome, molar ratio, 1 1 or 3 2) prepared by the dry-film method have been often used as non-vtral gene delivery vectors. We have shown that a more efficient transfection in medium with serum was achieved using DC-Chol/DOPE liposomes (molar ratio, 1 2) than those (3 2), and preparation method by a modified ethanol injection than the dry-film. The most efficient DC-Chol/DOPE liposome for gene transfer was molar ratio (1 2) and prepared by a modified ethanol injection method. The enhanced transfection is related to an increase in the release of DNA in the cytoplasm by the large lipoplex during incubation in opti-MEM 1 reduced-serum medium (optiMEM), not to an increased cellular association with the lipoplex. Cationic liposomes rich in DOPE prepared by a modified ethanol injection method will help to improve the efficacy of liposome vector systems for gene delivery. [Pg.393]

To avoid the difficulties of crossing the endothelium and nonspecific uptake by the liver, DNA can be delivered directly to the target tissue by injection of polyplexes, lipoplexes, or microspheres with encapsulated DNA. Injection of lipoplexes and polyplexes into the desired tissue can be used to achieve in vivo gene transfer. However, transfection depends on the physico-chemical properties of the complexes and the rate of clearance from the tissue, which varies from tissue to tissue and depends on factors such as the lymph supply. Nevertheless, polyplexes have been found to be sufficiently small and stable so as to diffuse throughout the brain ventricular spaces after a local injection injection [14]. [Pg.1015]

The use of responsive vectors highlights the multifaceted and complex nature of these cationic lipid vectors and the supramolecular structures they form with DNA. By varying composition, chain lengths, linkers, and various properties, the lipoplex phase can be tailored to improve gene delivery. The research featured in this chapter has shown how the use of responsive nonviral vectors can enhance endosomal escape of the lipoplex and release of DNA from the lipoplex system, subsequently affecting gene transfer. [Pg.3339]

Studies by Mechtler and Wagner [121] showed that for pLL-mediated gene transfer the use of acidic influenza peptide versions with specificity for endosomal acidic pH generated the best results. Such enhancing effects were also observed with other polymers, as described for example in [73,120]. For lipoplex-mediated gene transfer, less acidic variants gave better results [179]. [Pg.163]

Lipoplexes are commonly used in gene therapy to transfer genetic material into a cancer cell, either by suppressing oncogenes or activating tumor suppression control genes. Lipoplexes have also been used to successfully transfect respiratory endothelial cells leading to studies to treat respiratory diseases such as cystic fibrosis. [Pg.347]


See other pages where Gene transfer using lipoplexes is mentioned: [Pg.420]    [Pg.464]    [Pg.347]    [Pg.349]    [Pg.351]    [Pg.463]    [Pg.7]    [Pg.8]    [Pg.405]    [Pg.426]    [Pg.664]    [Pg.668]    [Pg.326]    [Pg.1031]    [Pg.1039]    [Pg.1161]    [Pg.1301]    [Pg.251]    [Pg.543]    [Pg.3475]    [Pg.69]    [Pg.160]    [Pg.797]    [Pg.331]    [Pg.332]    [Pg.261]    [Pg.1155]    [Pg.465]   


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