Gene transfer, nonviral vectors

Morozov, V.A., Noguiez-Hellin, P., Laune, S., Tamboise, E., Salzmann, J.L. and Klatzmann, D. (1997) Plasmovirus replication cycle of a novel nonviral/viral vector for gene transfer. Cancer Gene Ther., 4,286-293. 

Kim CK, Haider KH, Choi SH, Choi EJ, Ahn WS, Kim YB (2002) Nonviral vector for efficient gene transfer to human ovarian adenocarcinoma cells. Gynecol Oncol 84 85-93... 

Kim CK, Choi EJ, Choi SH, Park JS, Haider KH, Ahn WS (2003) Enhanced p53 gene transfer to human ovarian cancer cells using the cationic nonviral vector, DDC. Gynecol Oncol 90 265-272... 

Abdallah, B., Hassan, A., Benoist, C., et al. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain Polyethylenimine. Hum. Gene Ther. 7(16) 1947—1954. 1996. 

Therefore, to develop an in vivo gene transfer vector with high efficiency and low toxicity, the limitations of one vector system should be compensated by the strengths of another type of vector system. From this standpoint, we have developed a novel hybrid gene transfer vector by combining viral and nonviral vectors. 

Nonviral vectors are also under investigation. Among these are receptor-mediated gene transfers. By its very nature these would be highly tissue specific. Here particular glycoproteins would target specific organs such as the liver (Wu, 1991). Liposomal encapsulation was already mentioned. In vivo injection of DNA can afford a direct transfection into cells. This nonvector method holds particular promise for muscle cells (Wolff etal., 1990). 

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