Wheat gene transfer

Genetic modification of many dicotyledoneous crops such as potato using gene transfer via A. tumefaciens is, at present, an efficient and reliable technique the application of the antisense route to limit or neutralize the action of undesirable genes has also been applied successfully to potatoes. Conversely, for monocotyledoneous crops such as maize, wheat, and rice, transformation can be accomplished by the much less efficient particle gun technique. This technique has been improved, resulting in rapid progress in the development of maize and later wheat with improved agronomic properties and/or altered starch composition. 

Kloti, A., Iglesias, V. A., Wiinn, J., Burkhardt, P., Datta, S. K., and Potrykus, I. (1993) Gene transfer by electroporation into intact scutellum cells of wheat embryos. Plant Cell Rep. 12, 671-675. 

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