Sendai virus gene transfer

Y. Saeki, N. Matsumoto, Y. Nakano, M. Mori, K. Awai, and Y. Kaneda. Development and characterization of cationic liposomes conjugated with HVJ (Sendai virus) reciprocal effect of cationic lipid for in vitro and in vivo gene transfer, Hum. Gene Ther. 8 2133-2141 (1997). 

Figure 1 Procedure of gene transfer by Sendai virus (HVJ)-liposomes. DNA and nuclear proteins are incorporated into liposomes by vortex-ultrasonication-annealing method, and the liposomes are fused with UV-inactivated Sendai virus. The resulting fusigenic viral liposomes can fuse with cell membrane to introduce DNA and nuclear protein complex directly into the cytoplasm. Nuclear protein such as HMG-1 can enhance the expression of foreign DNA in the nucleus.

Y. Kaneda, Virus (Sendai virus envelopes) mediated gene transfer, Cell Biology A Laboratory Handbook (J. E. Celis, ed.) vol. 3. Academic Press, San Diego, 1994, pp. 50-57. 

Y. Yonemitsu, Y. Kaneda, R. Morishita, K. Nakagawa, Y. Nakashima, and K. Sueishi, Characterization of in vivo gene transfer into the arterial wall mediated by the Sendai Virus (Hemagglutinating Virus of Japan) liposomes An effective tool for the in vivo study of arterial diseases, Laboratory Investigation 75 313 (1996). 

Tomita, N., Higaki, J., Ogihara, T., Kondo, T and Kaneda, Y. (1994) A novel gene-transfer technique mediated by HVJ (Sendai virus), nuclear protein, and liposomes. Ca. Detec. Preven. 18(6), 485 491. 

Virus (Sendai Virus Enveiopes)-Mediated Gene Transfer... 

FIGURE 1 Procedure for gene transfer using HVJ-liposomes. DNA and nuclear protein (HMG-1) are enclosed in liposomes by vortexing and sonication, and the liposomes are treated with HVJ (Sendai virus). The resulting HVJ-liposomes deliver the DNA-HMG-1 complex into the cytoplasm, and the complex migrates rapidly into the nucleus. 

Several other gene transfer technologies have been tested in chnical trials or are in various stages of development. These include recombinant viruses such as vaccinia virus [168], herpes simplex virus [169], canarypox virus [170], fowlpox [171], and Sendai virus [172], and nonviral vectors such as the use of magnetofection [173], DNA dehvery with nanoparticles [174] or polymers [175,176], and DNA... 

Li HO, Zhu YF, Asakawa M, Kuma H, Hirata T, Ueda Y, Lee YS, Fukumura M, lida A, Kato A, Nagai Y, Hasegawa M. A cytoplasmic RNA vector derived fiom nontransmissible Sendai virus with efficient gene transfer and expression. J Virol 2000 74 6564-6569. 

Yonemitsu Y, Kitson C, Ferrari S, Farley R, Griesenbach U, Judd D, et al. ElEcient gene transfer to airway epithelium using recombinant Sendai virus. Nat Biotechnol2000 18 970-973. 

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