Skin tumors fibroblastic

Werth, V. P., Bashir, M. M., and Zhang, W., IL-12 Completely Blocks Ultraviolet-Induced Secretion of Tumor Necrosis Factor alpha from Cultured Skin Fibroblasts and Keratino-cytes, J. Invest. Dermatol. 120, 116-122, 2003. 

Kano, Y., and J. B. Little. 1989. Efficient immortalization by SV40 T DNA of skin fibroblasts from patients with Wilms tumor associated with chromosome lip deletion. Mol Carcinog 2(6) 314—21. 

Lymphocytes, platelets, endothelial cells, fibroblast, hepatocytes, astrocytes, intestine, Madin-Darby canine kidney (MDCK) cells, tracheal ciliary epithelia, choroid plexus, eye lens, frog skin, frog urinary bladder, Ehrlich ascites tumor cells Activation of KCI cotransport... 

Erythrocytes from humans, birds, and rat Ehrlich ascites tumor cells astrocytes fibroblasts C67 glioma cells intestine trachea parotid MDCK cells retinal pigment epithelium frog skin HeLa cells rabbit medullary thick ascending limb Inhibition of K+ and Cl channels... 

Recent developments in cellular biology have demonstrated the important role of mitogenic signal transduction in controlling the tumor proliferation. The induction of ornithine decarboxylase (ODC), PKC, protein kinase activities, and oxidative stress by the phorbol ester, TPA, is believed to be closely related to the tumor promotion activity of this compound. Topical application of green tea polyphenols to mouse skin was found to inhibit TPA-caused induction of ODC activity in a dose-dependent manner. Our studies demonstrated that EGCG and TF-3 inhibited TPA-induced transformation, PKC activation, and AP-1 binding activities in mouse fibroblast cells. ... 

All the steps necessary for cellular transformation and cancer induction were demonstrated in cultured human skin fibroblasts inducible AHH activity, altered cellular proliferation kinetics, and DNA damage (Milo et al. 1978). Thus, humans are likely to be susceptible to tumor induction by PAHs by these mechanisms. 

Patterns of mesenchymal differentiation in cutaneous neoplasms are no less diverse than those encountered in deeper soft tissues. Fibroblastic or myofibroblastic, fibrohistiocytic, muscular, neural, epithelial, and vascular lesions may be seen as primary tumors in the dermis and subcutis. As is also true in deep soft tissues, the histologic evaluation of those neoplasms may fail to provide an unequivocal diagnosis. Hence immunophenotyping has proven to be valuable in this context. The diagnostic separation of various spindle-cell, polygonal-cell, epithelioid-cell, and small-cell lesions of the skin is assisted in many settings by immunohistochemical analysis. 

The tnmor promotion activity of OA and DTX-1 was first observed in vivo on monse skin, initiated with 7,12-dimethylbenz[a]anthracene (DMBA), and snbsequently in rat glandular stomach, initiated with /V-methyl-M-nitro-/V-nitrosognanidine (MNNG)." " The in vivo tumor-promoting activity of OA and DTX-1 was confirmed also in in vitro cell cnltnres. In particular, exposure of mouse embryonic fibroblasts BALB/3T3 to the initiator 3-methylcholanthrene for 72 h, followed by treatments for 2 weeks with OA (12.4 nM or 24.8 nM) or DTX-1 (3.66 nM) led to transformation of cells. However, OA tetramethyl ether (34.7 nM) did not affect initiated cells transformation. Moreover, OA failed to indnce transformation of cells without 3-methylcholanthrene pretreatment and did not show initiating activity when cells were treated first with the toxin and then with the tumor promoter... 

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