Retinal pigmented epithelium cells

Bailey, KR, Ishida, BY, Duncan, KG, Kane, JP, and Schwartz, DM, 2004. Basal reverse cholesterol transport of retinal pigment epithelium cell digested photoreceptor outer segment lipids. Invest Ophthalmol Vis Sci 45, U721. 

Constable, PA, Lawrenson, JG, Dolman, DE, Arden, GB, and Abbott, NJ, 2006. P-Glycoprotein expression in human retinal pigment epithelium cell lines. Exp Eye Res 83, 24—30. 

Engelke M, Tykhonova S, Zorn-Kruppa M, Diehl H (2002) Tamoxifen induces changes in the lipid composition of the retinal pigment epithelium cell line D407. Pharmacol Toxicol 91(1) 13—21... 

Retinal pigment epithelium cells of Undulated membrane ... 

Hoppe, G. et al. Accumulation of oxidized lipid-protein complexes alters phagosome maturation in retinal pigment epithelium. Cell Mol. Life Sci. 61 (2004b) 1664-74. 

Toimela T, Tahti H, Salminen L (1995) Retinal pigment epithelium cell culture as a model for evaluation of the toxicity of tamoxifen and chloroquine. Ophthalmic Research 27 Suppl 1 150-153. 

Pollreisz, A., Afonyushkin, T., Oskolkova, O.V., Gruber, E, Bochkov, V.N., and Schmidt-Erfurth, U. 2013. Retinal pigment epithelium cells produce VEGF iu response to oxidized phospholipids through mechanisms involving ATF4 and protein kinase CK2. 

Lu, L., Garcia, C. A., and Mikos, A. G. (1998), Retinal pigment epithelium cell culture on thin biodegradable... 

Thomson, R. C., Giordano, G. G., Collier, J. H Ishaug, S. L., Mikos, A. G., Lahiri-Munir, D., and Garcia, C. A. (1996), Manufacture and characterization of poly(alpha-hydroxy ester) thin films as temporary substrates for retinal pigment epithelium cells. Biomaterials 17(3) 321-327. 

PHBV O2 plasma treatment Increased hydropilicity, reduces polymer surface roughness, resulting in high cell growth and attachments. A potential scaffold for retinal pigment epithelium cell culture. 86... 

Somatostatin receptor (sst1-5) activation in human retinal pigment epithelium cell cultures leads to cytostatic and apoptotic events... 

Headspace analysis with PTR-MS has also been used to good effect for the in vitro discrimination of cultured human cancerous and non-cancerous cell lines by Brunner etal. [3]. Two pairs of cancerous and non-cancerous human cell lines were used. The non-cancerous pair consisted of lung epithelium cells, A-549, and retinal pigment epithelium cells, hTERT-RPEl (cultured in different growth media). The second pair consisted of squamous lung... 

The retinal pigment epithelium cells (RPE) of the retina form an epithelial cell layer that takes up retinol from choroid capillaries and stores it as retinyl esters, to be used as substrate for the generation of 11-ds-retinal. In the layer of rod and cone photoreceptor cells adjacent to the RPE, 11-ds-retinal combines covalently with the protein opsin to generate the visual pigment rhodopsin in rods and, similarly, iodopsin in cones. Each rod outer segment is densely packed with some 10 molecules of rhodopsin per cell. The small quantity of vitamin A stored in the retina would be inadequate to maintain vision were it not for the visual cycle, a process in which 11-ds-retinal is regenerated after... 

Melanins are prodnced in mammals in two types of cells of different developmental origin (1) the melanocytes of the skin, hair, choroids and iris and (2) the retinal pigment epithelium (RPE). Specialized organelles of the melanocytes, the melano-somes, synthesize and store eumelanins and phaeomelanins. 

Seagle, B.L. et al., Melanin photoprotection in the human retinal pigment epithelium and its correlation with light-induced cell apoptosis, Proc. Natl. Acad. Sci. USA, 102, 8978, 2005. 

Ryeom, SW, Sparrow, JR, and Silverstein, RL, 1996b. CD36 participates in the phagocytosis of rod outer segments by retinal pigment epithelium. J Cell Sci 109, 387-395. 

D1 (10,17S-docosatriene) from DHA using tandem liquid chromatography-photodiode array-electrospray ionization-tandem mass spectrometry (LC-PDA-ESI-MS-MS)-based lipidomic analysis have been documented in ischemic brain [4] and retinal pigment epithelium [5], This new lipid is called neuroprotectin D1 (1) because of its neuro-protectiveproperties in brain ischemia-reperfusion [4] and in oxidative stress-challenged retinal pigment epithelial cells [5] (2) because of its potent ability to inactivate proapoptotic signaling (see apoptosis, Ch. 35) [5] and (3) because it is the first identified neuroprotective mediator derived from DHA. 

Figure 14.1 Schematic diagram of the blood-retinal barrier (BRB). The retinal cell layers seen histologically consist of retinal pigment epithelium (RPE) photoreceptor outer segments (POS) outer limiting membrane (OLM) outer nuclear layer (ONL) outer plexiform layer (OPL) inner nuclear layer (INL) inner plexiform layer (IPL) ganglion cell layer (GCL) nerve fiber layer (NFL) inner limiting membrane (ILM).

Sites of expression Ocular and extraocular photoreceptors Retinal pigment epithelium and Muller cells Ocular and extraocular photoreceptors... 

GFP gene under control of CMV to retinal cells by injection into the subretinal space of eyes in rats the GFP gene was efficiently expressed in both photoreceptor cells and retinal pigment epithelium predominant expression in photoreceptor cells was achieved using the rhodopsin promoter. The transduction efficiency was high and photoreceptor cells in >80% of the area of whole retina were expressing GFP (76). Intron-containing constructs have been successfully introduced into recent versions of lentivirus vectors (77). 

Philp, N.J., et al. 2003. Polarized expression of monocarboxylate transporters in human retinal pigment epithelium and ARPE-19 cells. Invest Ophthalmol Vis Sci 44 1716. 

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