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Human skin, cultures

Hamden, D. G. (I960), A human skin culture technique used for cytological examinations, Brit. J. Exp. Pathol. 41, 31-37. [Pg.242]

Moy LS, Howe K, Moy RL (1996) Glycolic acid modulation of collagen production in human skin fibroblast cultures in vitro. Dermatol Surg 22(5) 439-441... [Pg.175]

MWNTs were found to be cytotoxic in human skin fibroblasts (HSF42) and human epidermal keratinocytes (HEK) [42-44], whereas SWNTs were toxic in human keratinocyte (HaCaT) cultures [25, 26, 45]. Reduced cell proliferation and oxidative stress were reported also in epithelial (HeLa) cells [45] and murine epidermal cells (JB6 P + ) [46] upon incubation with SWNTs. [Pg.181]

In hirman keratinocyte cultures, triethanolamine was categorized as a weak inducer of a delayed (> 4 h) stimulation of the release of key mediators (arachidonic acid, eicosanoids, interleukin-la) that are known to be indicative of hyperproliferative and inflammatory events in human skin (Miiller-Decker et al., 1994). In line with the in-vitro irritancy tests, triethanolamine was found to be a non-irritant in a clinical patch testing study of human skin in 20 male volunteers (Miiller-Decker et al., 1998). [Pg.396]

Human skin fibroblasts are cultured from skin biopsy samples. The dermis is cut into small pieces (0.5 mm on each side) and placed in a dish containing DMEM, 10% (v/v) FCS, and 1% (v/v) antibiotic-antimycotic solution. When these primary cultures are confluent they are split into novel dishes. Cells between passages three and six are used for experiments. [Pg.519]

Human skin fibroblasts are cultured from skin biopsy samples. The dermis is cut into small pieces (0.5 mm on each side) and placed into a dish in DMEM containing 10% (v/v) FCS and 1% (v/v) antibiotic-antimycotic solution. When these primary cultures are confluent they are split and cells between passage three and six are used for experiments. For the cholesterol efflux assay, cells are grown in 24-well plates to 60-80% confluence and are labeled with [1,2-3H]-cholesterol (1 pCi/well) for 24 h. Cells are then washed with DMEM and incubated for 4 h at 37°C with DMEM containing BSA (0.2%, v/v) and either 0 (negative control) or 5-30 pg/ml apoA-I. The efflux medium is collected and centrifuged to remove cell debris. Cells are solubilized in 0.1 mol/1 NaOH and the radioactivity in the efflux media and in the cell lysates is determined by scintillation counting [11, 30, 75]. [Pg.532]

Beckman et al. (28) have studied the electrophoretic separation of the acid phosphatase activity in tissue extracts on starch gel at pH 8. They described four electrophoretic bands A, B, C, and D. Table IV (28) shows the distribution of activity in different organ extracts. The ABD pattern predominated in kidney BD in liver, intestine, heart, and skeletal muscle B in skin and D in pancreas. The C component was present in a large number of placentae but not in other adult organs. All four electrophoretic components were inhibited by d-(- -)-tartrate A contained sialic acid, D had a lower pH optimum and was more heat resistant than A, B, and C. Components C and D showed parallel electrophoretic behavior. In human skin fibroblasts grown in tissue culture, the acid phosphatase was generally high and the most common pattern was BD. Almost every culture showed some activity. The BD... [Pg.454]

Glycosaminoglycan synthesis, in culture, using fibroblasts from human skin,97,98 human normal and hypertrophic scar,99 and embryonic chick... [Pg.256]

K. Takigaki, T. Nakamura, A. Kon, S. Timura, and M. Endo, Characterization of /9-D-xyloside-induced glycosaminoglycans and oligosaccharides in cultured human skin fibroblasts. J. Biochem. (Tokyo), 109(1991)514-519. [Pg.261]

Comparison of Spatial and Molecular Properties of Human and Cultured Skin... [Pg.372]

In their initial studies, Tfayli et al. [33] acquired spectra from an Episkin model. This model is comprised of human adult keratinocytes which produce stratified epidermis following a 13 h culture period. Raman spectra from this model were compared with normal human skin. Significant differences were noted, particularly in spectral features arising from the 850/830 tyr Fermi doublet (which is sensitive to the H-bonding state of the -OH group [34]) and in the protein amide III region. Usable spectra were acquired to a depth of 15-20 pm. [Pg.373]

Fig. 15.5. Factor analysis results for the C-H stretching region (2800-3050 cm 1 region) in human skin and in cultured skin model (Epiderm ). Data from human skin (8 x 12 pixels) and cultured skin (7 x 12 pixels) have been concatenated. Pixels marked with x s were excluded from the analysis, a Factor loadings for the methylene stretching region. The dashed vertical line marks 2876 cm-1 and emphasizes the shift in frequency between factors 1 and 2. b Score plots for factor 1 are depicted for human skin in the left set of 8 X 12 pixels and for cultured skin in the right set of 7 x 12 pixels, c Score plots for factor 2 are depicted for human skin in the left set of 8 x 12 pixels and for cultured skin in the right set of 7 x 12 pixels... Fig. 15.5. Factor analysis results for the C-H stretching region (2800-3050 cm 1 region) in human skin and in cultured skin model (Epiderm ). Data from human skin (8 x 12 pixels) and cultured skin (7 x 12 pixels) have been concatenated. Pixels marked with x s were excluded from the analysis, a Factor loadings for the methylene stretching region. The dashed vertical line marks 2876 cm-1 and emphasizes the shift in frequency between factors 1 and 2. b Score plots for factor 1 are depicted for human skin in the left set of 8 X 12 pixels and for cultured skin in the right set of 7 x 12 pixels, c Score plots for factor 2 are depicted for human skin in the left set of 8 x 12 pixels and for cultured skin in the right set of 7 x 12 pixels...
Another striking difference between normal and cultured skin is shown in Fig. 15.6. As discussed above (see Fig. 15.3c, factor 2), cholesterol-rich pockets containing highly ordered lipid chains are occasionally detected in human skin and are characterized by a Raman-active mode of cholesterol near 700 cm-1 and an intense lipid C-C stretch near 1130 cm-1 in Fig. 15.4a and b, respectively. The intensity of the cholesterol mode is normalized to a Phe vibration near 620 cm-1 and imaged in Fig. 15.6b. As is evident there are many such pockets in the cultured skin model, in contrast to human skin where they are only rarely observed (Fig. 15.3c, factor 2), and usually in the viable epidermis rather than in the SC (as in the cultured skin). These measurements illustrate the power of confocal Raman microscopy for combining spatial measurements with molecular structure characterization. [Pg.374]

Hofland, H.E., et al. 1991. Interactions of non-ionic surfactant vesicles with cultured keratinocytes and human skin in vitro A survey of toxicological aspects and ultrastructural changes in stratum corneum. J Control Release 16 155. [Pg.275]

In the initial studies of trace elements of human skin cross sections, a Ca profile evolved which was increasing from the basal, germinative level of the epidermis toward the horny layer. The drastic concentration drop of Ca concentration down to threshold values at the border of the stratum germinativum and the stratum corneum was a particularly interesting feature.15-17 Only a few years later this finding could be correlated to the fact that a Ca concentration >0.1 mmol was essential if a fully cornified stratum corneum was to be obtained in cell culture. This relationship between Ca and terminal differentiation of the epidermal cells was verified in a PIXE study of epidermal cell cultures.18... [Pg.54]


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