Mouse embryonic fibroblast

The biological impact of starch capped copper nanoparticles on mouse embryonic fibroblast (3T3L1) cells in vitro) was also evaluated by various parameters. More than 85 % of the 3T3Llcells were found to be viable, even after 20 hours time exposure which implies minimum impact on cell viability and morphology. The study demonstrates dose dependent cytotoxic potential of SCuNPs, that is non cytotoxic in the nanogram dose and moderately cytotoxic in the microgram doses (Fig. 10). Comparison of SCuNPs with Cu ions and uncapped copper nanoparticles (UCuNPs) revealed that, ions are more cytotoxic than SCuNPs. This observation supports the theory of slow release of ions from starch coated nanoparticles. 

Fig. 10. Effect of starch capped copper nanoparticles on cell viability (MTT assay) in case of mouse embryonic fibroblast (3T3L1).

Fig. 11. Photomicrographs of mouse embryonic fibroblast (3T3L1) cells (A) untreated, treated with (B) control (C) SOOng CuNPs, (D) lOOOng CuNPs.

It is well known that 1,10-phenanthrolines are highly active ironchelating agents. The parent compound itself has recently been shown to increase HIF-la levels in ocular tissue and to suppress 02-mediated epithelial cell proliferation when administered to mice [29]. A quantitative assay was developed to measure transcriptional potency of certain HIF stabilizers via an HRE-mediated (3-lactamase production in which the EC50 of 1,10-phenanthroline was measured to be approximately 8 pM. In addition, VEGF was dose-dependently produced in mouse embryonic fibroblasts by 1,10-phenanthroline with an EC50 of... 

In vitro studies with unmodified and modified N-terminal peptides of H3 demonstrated that Lys-14 acetylation did not interfere with methylation at Lys-9 by Suv39hl, while phosphorylation at Ser-10 and acetylation at Lys-9 did (Fig. 7). Further dimethylation of Lys-9 reduced enzymatic activity [186], A Suv39h double null primary mouse embryonic fibroblasts had higher levels of Ser-10 phos-phorylated H3 than wild type cells. These mutant cells had increased numbers of micro- and polynuclei. Oversized nuclei were characteristic of subpopulation of cells. The level of Lys-9 methylated FI3 in wild type cells and Suv39h double null cells was similar, demonstrating that other FI3 methyltransferases were involved [195]. Phosphorylation of Ser-10 by Ipll/aurora was also studied. Acetylation at Lys-14 promoted the activity of the mitotic kinase, while dimethylation, but not acetylation at Lys-9, reduced activity of the kinase [186]. 

Irradiated wild type (WT) and CD 154 expressing (CD 154) NIH3T3 mouse embryonic fibroblasts (see Note 2) WT and CD154 NIH3T3 cells are cultured in complete DMEM and maintained in a humidified atmosphere of 5% CO at 37°C. 

Jchibler Bert Van der Horst did an experiment where he showed that he couldn t induce circadian rhythms in Crj double knockout mouse embryonic fibroblasts. This is not surprising because they probably need the same components than in the clock. My strong prediction would be that everything is flat in the periphery. 

Reconstitution of full-length BRCAl into mouse embryonic fibroblast cells with a disrupted BRCAl led to an increase in resistance to several DNA damaging agents, including the platinum compounds carboplatin and oxaliplatin, the topoisomerase 1 drugs irinotecan and topotecan, and the topoisomerase 11 drugs doxorubicin and etoposide (61). 

Another important discovery was made when comparing MVLBisG2 and DOTAP in a number of different cell lines. As shown in Fig. 12, complexes of MVLBisG2 efficiently transfect a variety of mouse and human cells in culture [24]. Their TE reaches or surpasses that of optimized complexes prepared from commercially available DOTAP. Most importantly, complexes containing MVLBisG2 are significantly more transfectant over the entire composition range in mouse embryonic fibroblasts (MEFs). MEFs are important as feeder cells for embryonic stem cells and are a cell line that is empirically known to be hard to transfect. 

Fig. 12 Transfection efficiencies for DOTAP/DOPC and MVLBisG2/DOPC complexes in four different cell lines, plotted against the mole fraction of cationic lipid. The data points were obtained at a constant pchg (7 for HeLa cells, 4.5 for all others), corresponding to a constant amount of DNA applied to the cells for each data point in a plot. Remarkably, MVLBisG2 complexes are significantly more transfectant in mouse embryonic fibroblasts, a cell line empirically know to be hard to transfect and of large practical importance as feeder cells for embryonic stem cells. Reprinted with permission from [24]. Copyright 2006 American Chemical Society...

Fibronectin, collagen Photoreactive PVA modified with phenylazido groups Monkey kidney fibroblasts, murine macrophage-like cells, human liver carcinoma cells, mouse embryonic fibroblasts tCP of proteins onto hydrogel background and subsequent photoactivated immobilization 2005 [90]... 

Although methods for silencing gene expression in worms and invertebrates were well established, researchers had few options for targeting specific genes within mammalian genomes. Prior to the advent of RNAi, an effective but costly method was to generate knockout mice that were used to produce knockout mouse embryonic fibroblasts (MEFs). 

Cells and cell lines NIH 3T3, mouse embryonic fibroblasts K562, human chronic myeloid leukemia MDA-MB-231, human breast cancer MiaPaCa2, human pancreatic cancer n/d, not determined NSCLC, nonsmall cell lung cancer p-, phosphporylated form of a protein RB, retinoblastoma protein. 

Sir2 family of NAD+-dependent protein deacetylases that is involved in a caloric restriction-dependent life span extension, has a strong preference for histone H4K16Ac in its deacetylation activity. Mouse embryonic fibroblasts (MEFs) deficient for SirT2 show higher levels of H4K16Ac in mitosis when compared with the normal levels exhibited by SirTl-deficient MEFs (21). The enzymatic conversion of H4K16Ac to its deacetylated form may be pivotal to the formation of condensed chromatin. 

Cell lines Pluripotent hESC H9.2 clone [56], mouse embryonic fibroblast (MEF) feeder layer. 

Mouse embryonic fibroblasts (MEFs) derived from SIP2 null mutant mice proved quite useful in further understanding the role SIP2 plays in SIP signaling. In response to SIP, MEFs derived from SIP2 null mutant mice showed a significant decrease in Rho activation but had relatively normal PLC, calcium mobilization, and AC responses (Ishii et al. 2002). 

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