Ethylenediamine tetra acetic acid

Part of the LPS may be removed by treating the cells with ethylenediamine tetra-acetic acid (EDTA) or related chelating agents (Chapter 12). 

Chelators, especially ethylenediamine tetra-acetic acid (EDTA), have been used as potentiators of the aetion of ehloroxylenol. Vaara has extensively reviewed the subject of permeabilization and Ayres, Furr and Russell have described a rapid method of evaluating the permeabilizahon of Ps. aeruginosa (see Further Reading, Sechon 6). 

CEC [Chisso Engineering Company] A process for removing oxides of nitrogen from flue-gases by scrubbing with an aqueous solution containing ferrous ion and ethylenediamine tetra-acetic acid (EDTA). An iron nitrosyl compound is formed. Developed by Chisso Engineering Company, Japan, and piloted in France and Japan. 

Geigy A one-stage process for making ethylenediamine tetra-acetic acid (EDTA) from eth-ylenediamine, hydrogen cyanide, and formaldehyde. 

Fig. 11. Binding of calmodulin to an array of a diverse set of 144 human proteins enables novel, calcium-dependent interactions to be identified. (A) Binding of Cy3-labeled anti-His tag antibody to the array allows the relative amount of protein in each spot to be determined. (B) Binding of Cy3-labeled calmodulin allows potential interacting partners to be identified. (C) Histogram showing the relative amount of calmodulin bound per unit protein in the presence of calcium ions. (D) Histogram showing the relative amount of calmodulin bound per unit protein in the presence of calcium ions and a high concentration of a divalent metal ion chelator, ethylenediamine tetra-acetic acid.

Fig. 8.3 The activity of different grain refiners (butanediamine, ammonium ethylenediamine tetra-acetic acid, benzosulfimide) on the nanostructure of gold deposits [29].

Electroless copper solutions contain copper salts and a reducing agent, such as formaldehyde the preparations for industrial use contain also stabilizers like 2-mercaptobenzothiazole, to prevent decomposition other than on activated areas of the workpiece, and accelerators like ethylenediamine-tetra-acetic acid—which increase the rate at which metal is deposited. Formulating such solutions requires achieving a balance between stability on the one hand and speedy deposition on the other. 

Reagents EDTA Disodium salt of ethylenediamine-tetra-acetic acid... 

Avoidance of interference of other milk constituents with measurements is also of importance for example, dissociation of casein micelles by calcium-chelating agents, such as trisodium citrate or ethylenediamine tetra-acetic acid (EDTA), may used to avoid interference of the micelles in particle size measurement, while clusters of fat globules can be disrupted by adding a low level of sodium dodecyl sulphate (SDS). 

MODIFICATIONS OF THE METHOD It can be appropriate to coincubate the compound of interest in the presence of inhibitors of serum esterases. Used is sodium fluoride, physostigmin or ecothiophate iodide (Chien 1990 Quon et al. 1993). In case of carboxy- or aminopeptidase cleavage of peptides specific peptidase inhibitors like amastatin, bestatin, phenylmethylsulphonylfluoride, 1,10-phenanthroline or ethylenediamine tetra acetic acid (EDTA) are useful (Lee 1995). 

Iron should be coordinated by the chelate in such a manner as to prevent direct access of oxygen and hydrogen peroxide. If this is achieved, then hydroxyl-radical production will be reduced to a minimum. Some iron complexes, for instance ethylenediamine tetra-acetic acid (EDTA) (Fig. 3a), generate hydroxyl radicals efficiently while others such as DFO and the hydroxypyridinones (Fig. 3b) do not. By designing chelators that produce extremely stable complexes, the generation of hydroxyl radicals is further minimized. Such stable complex formation would also reduce the tendency for iron redistribution within the body. 

Complex formation reactions, in which the reactants are combined to form a soluble ion or compound. The most important reagent for formation of such complexes is ethylenediamine tetra-acetic acid, EDTA (as the disodium salt). 

Numerous tertiary amines that also contain carboxylic acid groups form remarkably stable chelates with many metal ions. Ethylenediamine tetra-acetic acid (EDTA) can be used for determination of 40 elements by direct titration using metal-ion indicators for endpoint detection. Direct titration procedures are limited to metal ions that react rapidly with EDTA. Back titration procedures are useful for the analysis of cations that form very stable EDTA complexes and for which a satisfactory indicator is not available. EDTA is also used for determining water hardness the total concentration of calcium and magnesium expressed in terms of the calcium carbonate equivalent. 

Bhushan M, Beck MH. Allergic contact dermatitis from disodium ethylenediamine tetra-acetic acid (EDTA) in a local anaesthetic. Contact Dermatitis 1998 38(3) 183. 

Ethylenediamine tetra-acetic acid is a chelating agent that is used as an anticoagulant for collecting blood samples and for removing calcium from experimental fluids in laboratories. 

Systemic contact dermatitis is a delayed hypersensitivity skin reaction that results from systemic exposure. Exanthematous systemic contact dermatitis from ethylenediamine has been reported with aminophylline. Disodium edetate (ethylenediamine tetra-acetic acid) has caused contact dermatitis after local application (SEDA-23, 242), and ethylenediamine cross-reacted in a patch test in a patient who had had contact dermatitis with hydroxyzine, an ethylenediamine derivative (SEDA-22, 178). Prior sensitization can occur to ethylenediamine in creams and ointments (SED-14, 485). 

Glassware should be baked overnight at 200°C. Nondisposable plasticware should be rinsed thoroughly with 0.1 A NaOH, 1 mill ethylenediamine-tetra-acetic acid (EDTA), and then with RNase-free (diethylpyrocarbonate [DEPC]-treated) water. Alternatively, it can be treated with RNase AWAY (Molecular BioProducts), according to the manufacturer s instructions. 

Some TCA masks have been presented as chelated TCA. Chelation is a medical therapy that aims to detoxify the body of harmful minerals and metals. Chemically, chelation is the process by which an organic substance (the chelator) binds metal ions (iron, copper, lead, calcium, etc.) into inactive, non-toxic and water-soluble complexes that are easily eliminated in the urine. Intravenous chelation therapy (e.g. with ethylenediamine tetra acetic acid, EDTA) is often used to treat poisoning with heavy metals, including lead. The use of the term chelation therefore seems inappropriate as far as TCA is concerned, and has no chemical basis since TCA is not a metal. The little information available states that chelation reduces the speed of penetration of TCA and therefore its depth of action. Might what we call chelation be partial inactivation of the TCA The directions of use for Accu Peel state that a process called chelation allows the TCA to reach an even depth at the same time as using lower concentrations of TCA . Might chelation, on the contrary, be a process that activates the TCA But how can TCA be activated ... 

Figure 15. Polyreaction of ethylenediamine tetraacetic acid dianhydride (1) and D-lactose (2), yielding the poly(ethylenediamine tetra-acetic acid-co-lactose) (3).

The retention profiles of the water-soluble polymer the poly (ethylenediamine tetra-acetic acid-co-lactose) clearly show that the polymer has a strong binding ability for iron and chromium, as it exhibited a strong metal complexation with Cr(III) and Fe(III) with retention values of 100%. In contrast, Co(II), Ni(II), Cu(II), Zn(II), Sr(III), Cd(II), Pb(II), and Al(III) showed lower retention values (20-80%). Thus, the enrichment of Cr(III) and Fe(III) from the other metal ions can be achieved. 

If a copper after-treated dyeing is too heavy it can be stripped partially by boiling in a liquor containing 1 to 3 g per litre of ethylenediamine-tetra-acetic acid, an anion-active detergent, and 1 g per litre of sodium carbonate. This treatment removes the copper, and when the shade has been brought down to the desired depth it must be recoppered. If a complete strip is necessary the copper is removed by treatment for 30 minutes at 90°C (194°r) with 1 to 3 per cent of ethylenediaminetetra-acetic acid, followed by treatment at S0°C (176°F) with a solution containing 3 to 5 g per litre of sodium carbonate and 2 to 3 g per litre of sodium hydrosulphite. 

CE CGE CHOL CIP CPG CTAB CZE dA, dG, dC DBU DEAE DMF DMT DNP DOPE DOTMA EDTA EM EOF ESI-MS Fmoc FPE ICAM-1 Capillary electrophoresis Capillary gel electrophoresis Cholesterol Cahn-Ingold-Prelog nomenclature system for absolute configuration Controlled pore glass Hexadecyltrimethylammonium bromide Capillary zone electrophoresis Deoxyadenosine, deoxyguanosine, deoxycytosine l,8-Diazabicyc o[5.4.0]undec-7-en Diethylaminoethyl- Dimethylformamide Bis(4-methoxyphenyl)phenylmethyl-, (syn. Dimethoxytrityl-) 2,4-DinitrophenyI- Dioleylphosphatidylethanolamine N-[l-(2,3-dioleyloxy)propyl]-N, N, N-trimethylammonium chloride Ethylenediamine tetra-acetic acid Electrophoretic mobility Electro-osmotic flow Electrospray ionization mass spectrometry 9-Fluorenylmethoxycarbonyl-Fluid phase endocytosis Intracellular Adhesion Molecule-1... 

QCA = quinoline-2-carboxylic acid NOC = nocardamine EDTA = ethylenediamine tetra acetic acid NT A = nitrilotriacetic acid TRIS = tris(hydroxymethylamine) methane. 

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