Ehrlich ascites tests

On day zero,.mice weighing approximately 20 g were inoculated with 5 X 10 Ehrlich ascites tumor cells (EATC) SEM = standard error of the mean. All test solutions were prepared in Krebs Ringers Phosphate buffer and administered by i.p. injection on days 0, 1, 3, 5, 7, 9, 12, 15, and 18. 

Recently we have thiolated nucleic acid fractions isolated from Ehrlich ascites (EA) cells. The activities of thiolated DNA, ribosomal RNA and transfer RNA were studied in a DNA-polymerase system from Friend leukemia virions. Of all the fractions tested transfer RNA, thiolated 1-3% showed a maximum inhibition of DNA polymerases of oncorna viruses. Table 23 shows some of the results obtained using thiolated tRNA. 

The above described rationale of SAR can be exemplified by the bioactivities of the bromotyrosine derivatives, aeroplysinin-1 and dienone, as well as their complicated molecular substrates aerophobin-1 and isofistularin-3, isolated from the Mediterranean sponge, Aplysina aerophoba. Aeroplysinin-1 and dienone exhibit pronounced biological activity whereas aerophobin-1 and isofistularin-3 were always inactive when tested in equimolar concentrations [23]. Aeroplysinin-1 and dienone are antibiotically active against a broad spectrum of marine bacteria such as Vibrio, Micrococcus, and Alteromonas species [24] and are also cytotoxic to Ehrlich ascites tumour cells and HeLa tumour cells in the microculture tetrazolium (MTT) and clonogenic assays [25]. In these assays, it has been demonstrated that the free radical transformation of aeroplysinin-1 and dienone to its semiquinone structures are responsible for the cytotoxicity [25], Aeroplysinin-1 and dienone are the pharmacophores of aerophobin-1 and isofistularin-3. 

The effect of 2-[2-(5-nitro-2-furyl)vinyl]quinoline (LIX) and 20 other compounds on Ehrlich ascites carcinoma EY 33 in mice was tested against Miomycin G in 1964 . Pure strain healthy mice were intraperitoneally inoculated with this tumour. In general the animals will die of the accumulation of tiscites after 10-19 days. Compounds are considered effective when treated mice survive 50 days after the inoculation of the tumour. The compounds found to be effective in this test were the compound LIX), the sodium salt of 2-(5-nitro-2-furyl)vinylquinoline-4-carboxylic acid LXII),... 

Tumor-inhibiting against mouse P388 lymphocytic leukemia and in Ehrlich ascites tumor-test... 

Skold 20) purified this enzyme 400-fold from Ehrlich ascites tumor cells and demonstrated the above stoichiometry and magnesium requirement. Various nucleosides were tested as substrates and the results were as follows ... 

All T) -cyclopentadienyl metal complexes listed in Tables 1-3 were investigated against fluid Ehrlich ascites tumor, and antitumor activity of varying strength was detected (Tables 5-7). Some of those compounds, which were characterized by pronounced tumor-inhibiting activity against Ehrlich ascites tumor, were additionally tested against other fluid and solid animal tumors. 

Within the group of ferricenium salts, antitumor activity against solid Ehrlich ascites tumor was tested for the compounds listed in Table 9. They induced less pronounced growth inhibitions against this tumor system than I and II, whereby tumor suppression by about 50% was effected by the tetrachloroferrate, n-oxo-bis(trichloroferrate) and the tiichloroacetate derivatives XLIX, LI and... 

The products were tested by the mouse P388 lymphocytic leukemia survival test and by the Ehrlich ascites tumor regression test. The polyphosphazene-plat-inum product showed an inhibition of 86% in the ascites test and a 5/7 survival after the eighth day for the P388 mouse test. 

Furthermore, mouse model against Ehrlich s ascites tumour (EAT) [36]. This study showed that the treatment with ama-titano-cene 48 increases the lifespan of EAT-bearing mice from 25% to around 50% in a dose-dependent manner, and myelopoiesis (the formation of bone marrow or of blood cells derived from bone marrow) was not suppressed. Additionally, these experiments showed that ansa-titanocene 48 restores the natural killer cell function, which is reduced due to a dysfunction in EAT, and stimulates the natural killer cell-mediated cytotoxicity. 

In vitro cytotoxic activity of calendula extracts was observed in MRC5, Hep2, and Ehrlich cell lines. When the same extracts were tested in mice, one was inactive and three were poorly active, whereas the most saponin-rich extract did not produce development of ascites (Boucaud-Maitre et al. 1988). 

CijHzoOj, Mr 248.32, cryst., mp. 99°C, [aJo -154° (CHjOH). A sesquiterpenoid of the isolactarane type from cultures of Merulius tremellosus (Basidiomy-cetes). M. is active against bacteria, fungi, and yeasts. It also has cytotoxic activity against ascites cells of the Ehrlich carcinoma and shows strong mutagenicity in the Ames test. 

---