Microculture tetrazolium

Alley, MC, Scudiero DA, Monks A, Hursey ML, Czerwinsk MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH Boyd MR (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research 48 589-601. 

The above described rationale of SAR can be exemplified by the bioactivities of the bromotyrosine derivatives, aeroplysinin-1 and dienone, as well as their complicated molecular substrates aerophobin-1 and isofistularin-3, isolated from the Mediterranean sponge, Aplysina aerophoba. Aeroplysinin-1 and dienone exhibit pronounced biological activity whereas aerophobin-1 and isofistularin-3 were always inactive when tested in equimolar concentrations [23]. Aeroplysinin-1 and dienone are antibiotically active against a broad spectrum of marine bacteria such as Vibrio, Micrococcus, and Alteromonas species [24] and are also cytotoxic to Ehrlich ascites tumour cells and HeLa tumour cells in the microculture tetrazolium (MTT) and clonogenic assays [25]. In these assays, it has been demonstrated that the free radical transformation of aeroplysinin-1 and dienone to its semiquinone structures are responsible for the cytotoxicity [25], Aeroplysinin-1 and dienone are the pharmacophores of aerophobin-1 and isofistularin-3. 

Marshall, N. J., Goodwin, C. J., Holt, S. J. 1995. A critical assessment of the use of microculture tetrazolium assays to measure cell growth and fimctioiL Growth Regulation 5 69-84. 

Cellular growth in the presence or absence of experimental agents was determined using the modified method as previously described MTT -microculture tetrazolium assay 10), Briefly, rapidly growing cells were harvested, counted, and inoculated at the appropriate concentrations (100 pL volume) into 96-well micro-titer plates using a multichannel pipet. After 24 hours, AO-1 to AO-5 were applied in different concentrations to triplicate... 

Cell proliferation inhibition. Cytotoxicity was determined as described previously34 by the colorimetric MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (Fluka) microculture assay on compounds freshly dissolved in DMSO... 

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