Drosophila characterization

Kurth P, Preiss A, Kovall R A, Maier D. Molecular analysis of the Notch repressor-complex in Drosophila characterization of potential hairless binding sites on suppiessm- of haMess. PLoS One. 2011 6(1 l) e27986. doi 10.1371/joumal.pone.0027986. 

Several nonconventional cadherins that contain cadherin repeats have been described but they have specific features not found in the classical cadherins [1]. The cadherin Flamingo, originally detected in Drosophila, contains seven transmembrane segments and in this respect resembles G protein-coupled receptors. The extracellular domain of Flamingo and its mammalian homologs is composed of cadherin repeats as well as EGF-like and laminin motifs. The seven transmembrane span cadherins have a role in homotypic cell interactions and in the establishment of cell polarity. The FAT-related cadherins are characterized by a large number of cadherin repeats (34 in FAT and 27 in dachsous). Their cytoplasmic domains can bind to catenins. T- (=truncated-)cadherin differs from other cadherins in that it has no transmembrane domain but is attached to the cell membrane via a glycosylpho-sphatidylinositol anchor. 

Wnt/non-P-catenin signaling is not as well characterized biochemically as the Wnt/p-catenin-dependent pathway this may reflect that it is molecularly more diverse, at least in vertebrates. In Drosophila the best characterized Wnt/non-P-catenin pathway is planar cell polarity (PCP) signaling. Ironically, although it certainly depends on a Fz receptor, it remains... 

LRP5 and 6 are not required for Wnt/non-(3-catenin signaling instead a protein called Ryk (derailed in Drosophila) is the best characterized coreceptor for the Fz proteins in non-(3-catenin signaling. Ryk/derailed was first implicated in axon guidance, but could conceivably play a more general role in all forms of Wnt/non-P-catenin signaling controlling cell shape, movement, and polarity. 

SERCA-type -ATPases from non-mammalian cells (SERCAMED) Sequences of SERCA-type Ca -ATPases were also obtained from Plasmodium yoelii [68], Anemia [69] and Drosophila [70], These enzymes are similar in size to the SERCAl- and SERCA2a-type Ca -ATPases from mammalian muscles, but based on their N- and C-terminal sequences they represent a distinct group. In spite of the wide philogenetic variations between them they all share a common N-term-inal sequence (MED) that differs from mammalian enzymes. None of the corresponding proteins were isolated and characterized. 

At this time, more then thirty channel DNAs have been cloned and characterized from various sources, predominantly from Drosophila melanogaster, mouse, rat and human cDNA/genomic libraries [6-31]. Inspection of the derived primary K channel protein sequences indicates that voltage-gated channels belong to a... 

Witz, P., Amlaiky, N., Plassat, J. L., Maroteaux, L., Borrelli, E., and Hen, R. (1990). Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase. Proc. Natl. Acad. Sci. USA 87 8940-8944. 

Goncyy Their molecular nature is completely different. PAR-3 is a PDZ-containing protein that has Bazooka and ASIP as Drosophila and mammalian cousins, respectively (Izumi et al 1998, Kuchinke et al 1998). PAR-2 is a ring-finger containing protein that does not have a characterized Drosophila or mammalian orthologue. 

Gnagey, A.L., Forte, M. and Rosenberry, T.L. (1987) Isolation and characterization of acetylcholinesterase from Drosophila. Journal of Biological Chemistry 262, 13290-13298. 

Ogawa E, Maruyama M et al. Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila mnt-related DNA binding protein PEBP2 alpha. Virology 1998 194 314-331. 

The role of PARP-1 as a modulator of chromatin stiucture, as well as its chromatin-dependent effects on transcription, has been well established in the literature (D Amours et al, 1999 Kraus and Lis, 2003 Rouleau et al, 2004 Kim et al, 2005). In this section, we will highlight some of the historical studies, as well as more recent studies, that have characterized the role of PARP-1 and PAR in these processes. Note that, when describing this work, the term PARP in the absence of any additional identifiers refers to PAR-generating proteins of ambiguous identity (e.g., as in early studies, which were conducted without the knowledge that multiple PARP family members exist, or when multiple splice variants of a PARP-1-like protein exist in a single organism, as in Drosophila). 

Elfring, L.K., Deuring, R., McCallum, C.M., Peterson, C.L., and Tamkun, J.W. (1994) Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2. Mol. Cell. Biol. 14, 2225-2234. 

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