Plant DNA

Tomato DNA Plant Technology/1994 A fragment of the gene encoding aminocyclopropanecarboxyUc acid synthase (ACCS) to suppress the endogenous ACCS enzyme Tomato Delayed ripening due to reduced ethylene synthesis... 

Codex Alimentarius Commission. 2003. Alinorm 03/34 Appendix III. Guideline for the conduct of food safety assessment of foods derived from recombinant DNA plants. Annex IV. Annex on the assessment for possible allergenicity, Rome, Italy. 

The mucosal vaccines approved for human use include typhoid, cholera, adenovirus, Sabin oral polio, and rotavirus vaccines. New mucosal vaccine strategies are focused on development of non-replicating subunit vaccines, DNA, plant, and other types of recombinant vaccines as well as the use of mucosal adjuvants preferably inbuilt into the vaccine. The conjugation of lipids to peptide antigens is one approach which enables the production of highly... 

Campbell, W. H., 1992, Expression in Escherichia coli of cytochrome c reductase activity from a maize NADH nitrate reductase complementary DNA, Plant Physiol. 99 693fi... 

DNA Plant Technology Corporation, 2611 Branch Pike, Cinnaminson, NJ 08077... 

PPJ Dunn and JC Gray (1988) Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA. Plant Mol Biol 11 311-319... 

Increasing the solids content of tomato is a demonstration of the modification of multigenic traits by modern biotechnology. Although various institutions have utilized several approaches, only somaclonal variation has proven successful, with DNA Plant... 

Service, 29,65,84,125,140,203,355 Agricultural University, Netherlands, 250 ARO, The Volcani Cotter, 55 Clemson University, 346 Cornell University, 98,149 DNA Plant Technology Corporation, 381 Indiana State University, 44 Michigan State University, 111 National Chung Hsing University, 319 New York State Agricultural Experiment Station, 98... 

Figure 8.18. Plot of mC against the GC levels of plant DNAs. Plants investigated by Matassi et al. (1992) as well as Arabidopsis are indicated. Data for other plants are from references given in the original paper. The correlation coefficient was 0.61 (p=0.0028). (From Matassi ct ah, 1992).

Wakasugi T, Sugita M, Tsudzuki T et al. Updated gene map of tobacco chloroplast DNA. Plant Mol Biol Rep 1998 16 231-241. 

DNA/plant junction sites in transformed tobacco plants. Although described for binary vector-mediated transformation, the method could be used to determine the number of integrations of Ri T-DNA into transformed root lines. 

Taberlet, P, L. Gielly, G. Pautou, and J. Bouvet, 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol, 17 1105-1109. 

The purpose of molecular characterisation is to inform the risk/safety assessment of plants derived from modem biotechnology. Such characterisation provides knowledge at the molecular level of the inserted DNA within the plant genome, the insertion site and the expressed material (ribonucleic acid [RNA] and proteins), and may provide information on intended and possible unintended effects of the transformation. Molecular characterisation of the genotype contributes to a rigorous assessment of the potential impacts of transformation on the food, feed and environmental risk/safety of a recombinant-DNA plant. It assists in the prediction of the phenotype and the phenotype will ultimately determine whether the recombinant-DNA plant poses any risk/safety concerns. ... 

This document focuses on the subset of recombinant-DNA plants intended for commercialisation, unconfined or full release that is subject to risk/safety assessments. 

Other terms such as genetically modified plants, genetically engineered plants, transgenic plants and transformed plants are often used interchangeably with the term recombinant-DNA plant. For the purposes of this document, the term recombinant-DNA plant will be used specifically as defined in paragraph 4. 

In conclusion, molecular characterisation is considered an important part of risk/safety assessment however it is only one component in the overall approach to risk/safety assessment. Molecular characterisation complements other components of the risk/safety assessment, such as environmental, chemical, nutritional, allergenicity and toxicological data to compare the recombinant-DNA plant with its appropriate comparator. Of interest for the risk/safety assessment is whether plant transformation could inadvertently increase the potential toxicity or allergenicity of the recipient plant. 

Agrobacterium-mediated transformation of plant tissue usually results in a low copy number of the DNA construct at a single insertion site. In some recombinant-DNA plant varieties reaching commercialisation T-DNAs have been found to be inserted as tandem repeats (direct or inverted instracture) at a single locus (reviewed by Smith et al., 2001). Integration of incomplete T-DNA... 

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