Tobacco chloroplasts

Human growth hormone produced in tobacco chloroplasts 11... 

Molina, A., Hervas-Stnbbs, S., Daniell, H., Mingo-Castel, A.M., and Veramendi J. (2004). High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotechnol J. 2(2) 141-153. 

Molina, A., Veramendi, J., and Hervas-Stnbbs, S. (2005). Indnction of nentral-izing antibodies by a tobacco chloroplast-derived vaccine based on a B cell epitope from canine parvovirns. Virology 342(2) 266-275. 

Another vaccine protein that has recently been produced in tobacco chloroplasts is the Fragment C domain of tetanus toxin (TetC). TetC is a nontoxic 47 kDa polypeptide that can induce an immune response upon... 

Daniell, H., Lee, S.-B., Panchal, T., and Wiebe, P.O. (2001). Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J. Mol. Biol. 311 1001-1009. 

His-tagged GUS-fusion proteins have been produced and isolated from tobacco chloroplasts. His-tagged proteins have also been extracted by foam frachonahon (Crofcheck et al., 2003,2004) or by a modihed intein expression system (Morassutti et al., 2002). 

Canine parvovirus Tobacco chloroplasts Epitope 2L21 Molina et ah, 2005 —continued... 

Staub JM, Garcia B, Graves J, et al. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nature Bio-technol., 2000 18(3) 333-338. 

Since the first successful transformation of tobacco chloroplasts [83], expression systems based on the transformation of plant plastids has attracted the attention of plant biotechnologists. The features that make this technology so attractive are its potential for high protein yield, along with inherent biosafety features such as limited plastid transfer via pollen (due to maternal inheritance of plastid-encoded genes) [84] and the relatively low probability of transgene movement from the chloroplast to the nucleus [85, 86]. 

Many other heterologous proteins have been expressed in tobacco chloroplast, and in many cases yields exceeding 5% of TSP were reported (Table 6.2). Examples of such proteins are neomycin phosphotransferase... 

Deoxyribonucleic acid, (tobacco chloroplast clone L27-1 ribosome protein L 27 messenger RNA-complementary plus 5 - and 3 -flanking region fragment) 950b, 1124b, 4249... 

Protein (tobacco chloroplast clone pTB24 gene psbK) 2530b, 4249... 

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