Plant transformation

Table 13.6 Higher plants transformed with carotenoid genes...

Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucl Acid Res 12 8711-8721... 

There are several methods that can be used to introduce foreign genes into plant cells, a process called, in general, transformation. Among the most common plant transformation methods are biolistics and exposure to Agrobacterium tumefaciens. 

An alternative procedure for plant transformation was introduced in 1987, involving the use of a modified shotgun to accelerate small (1—4 pm) metal particles into... 

Filipecki, M. and Malepszy, S. (2006). Unintended consequences of plant transformation a molecular insight. J. Appl. Genet. 47(4) 277-286. 

Rakoczy-Trojanowska, M. (2002). Alternative methods of plant transformation a short review. Cell Mol. Biol. Lett. 7(3) 849-858. 

Describes the theory and practice of modern plant transformation techniques with respect to nuclear and plastic genomes... 

Gelvin, S. B., 2003, Agrobacterium-mediated plant transformation the biology behind the "gene-jockeying" tool, Microbiol. Mol. Biol. Rev. 67 16-37. 

McBride, K. Summerfelt, K. (1990). Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Molecular Biology 14, 269-76. 

Fig. 4. Constitutive transcription of Gmhspl7.6-L mRNA in transgenic tobacco plants. The constructs A, B and C shown in Fig. 3 have been used for transformation of tobacco. Total RNA was isolated from leaves of individual plants which had been subjected to heat stress (hs) for 2 h at 40 °C, or incubated only at 25 °C (c). 30 xg RNA per lane were separated by electrophoresis and blots were hybridised with a gene-specific DNA probe (Schoffl et al., 1987). A,B,C refer to transgenic plants transformed with the chimaeric genes depicted in Fig. 3. C, and Q represent two individual plants. RNAs from soybean (sb) and untransformed tobacco (tob) were used as references.

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