PCR-denaturing gradient gel

Koizumi, Y. Kelly, J.J. Nakagawa, T. Urakawa, H. El-Fantroussi, S. Al-Muzaini, S. Fukui, M. Urushigawa, Y. Stahl, D.A. Parallel characterization of anaerobic toluene- and ethylhenzene-degrading microhial consortia hy PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization, and DNA microarray technology. Appl. Environ. Microbiol. 2002, 68, 3215-3225. 

Meroth, C.B., Walter, J., Hertel, C., Brandt, M.J., and Hammes, W.P. 2003. Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 69, 475-482. 

Cocolin, L., Manzano, M., Aggio, D., Cantoni, C., Comi, G. (2001). A novel polymerase chain reaction (PCR) - denaturing gradient gel electrophoresis (DGGE) for the identification of Micrococcaceae strains involved in meat fermentations. Its application to naturally fermented Italian sausages. Meat Science, 58, 59-64. 

Meroth, C. B., Hammes, W. P, Hertel, C. (2003). Identification and population dynamics of yeasts in sourdough fermentation processes by PCR-denaturing gradient gel electrophoresis. Applied and Environmental Microbiology, 69, 7453-7461. 

Kim, T. W Lee, J. H Kim, S. E., Park, M. H Chang, H. C Kim, H. Y. (2009). Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis. International Journal of Food Microbiology, 131, 265-271. 

Identification of the species and characterization of the dominant strains in static surface acetic acid fermentation were required for the stabilization of fermentation procedures and improvement of the strain. Recent advances of genetic analyses including DNA—DNA hybridization, enterobacterial repetitive intergenic consensus (ERlC)-polymerase chain reaction (PCR) and repetitive extragenic palindromic (REP)-PCR, random amplified polymorphic DNA (RAPD), and 16S rRNA sequence analysis with PCR-denaturing gradient gel electrophoresis (DGGE) methods allow identificatiOTi of species of acetic acid bacteria. 

Guan, L.L., Hagen, K.E., Tannock, G.W., et al. (2003) Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis. Appl Environ Microbiol 69, 6750-6757. 

Endo, A. and Okada, S. (2005) Monitatng the lactic acid bacterial diversity during shochu fermentation by PCR-denaturing gradient gel electrojfroresis. J Biosci Bioeng 99, 216—221. 

G. A. Kowalchuk, J. R. Stephen, W. DeBoer, J. 1. Prosser, T. M. Embley, and J. W. Woldendorp, Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified I6S ribosomal DNA fragments. Appl. Environ. Microbiol. 6.1 1489 (1997). 

Denaturing gradient gel electrophoresis (DGGE), real-time PCR, and FISH... 

Duineveld BM, Kowalchuk GA, Keijzer A, van Elsas JD, van Veen JA (2001) Analysis of Bacterial Communities in the Rhizosphere of Chrysanthemum via Denaturing Gradient Gel Electrophoresis of PCR-Amplified 16S rRNA as Well as DNA Fragments Coding for 16S rRNA. Appl Environ Micrbiol 67 172-178... 

Several rapid and sensitive methods have been developed to detect mutations in cDNA and genomic DNA. These include ribonuclease (RNase) protection analysis, denaturing gradient gel electrophoresis, and single-strand conformation polymorphism analysis. These methods can be used in conjunction with the PCR technique to detect small deletions or insertions and single base substitutions. 

Teske A., Wawer C., Muyzer G., and Ramsing N. B. (1996) Distribution of sulfate-reducing bacteria in a stratified fjord (Manager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments. Appl. Environ. Microbiol. 62, 1405—1415. 

One molecular method useful for the evaluation of microbial diversity is the apphcation of the Temporal Temperature Gradient gel Electrophoresis (TTGE) or the Denaturing Gradient Gel Electrophoresis (DGGE) to a PCR product (Myers et al. 1987). 

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