DNA extraction method

Sato Y, Sugie R, Tsuchiya B, et al. Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalin-fixed and paraffin-embedded tissues. Diagn. Mol. Pathol. 2001 10 265-271. 

The MSI is used as a diagnostic criterion of replication errors caused by various mutations in at least five mismatch repair genes (Lynch and Smyrk, 1996). Therefore, MSI analysis is useful in clinical practice to identify patients with hereditary nonpolyposis colorectal cancer. Raedle et al. (1999) have presented a rapid DNA extraction method (rapid microsatellite analysis) for analyzing replication errors in paraffin-embedded tissues. 

Forensic Science Introduction DNA Extraction Methods in Forensic Analysis Polymerase Chain Reaction in the Forensic Analysis of DNA... 

Coyle, H. M., Shutler, G., Abrams, S., Hanniman, J., Neylon, S., Ladd, C., et al. (2003). A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP). analysis. J. Forensic Sci. 48, 343-347. 

The second approach to storage of plant tissue is to minimize or even eliminate storage in ultracold fieezers and to store only the DNA permanently. Plant tissue is placed in the refrigerator as it arrives, and then DNA extraction is done as soon as possible. This is the preferred method (e.g., J. Palmer laboratory)11 in studies where fast DNA extraction methods work well, tissue can be processed quickly, and no backup tissue source for additional extractions of either DNA or proteins is needed or expected. The benefits of this approach include the following (1) the need is diminished for ultracold storage space, which is costly in terms of purchasing, electrical power, and maintenance (2) space is then available for more... 

The DNA extraction method is an in-house developed protocol. Microdissected tumor area are dipped into xylene to remove paraffin, rehydrated in a series of ethanol, and incubated in a proteinase K digestion buffer. Two different DNA purification methods are used. The first method is a NaCl saturated solution precipitation, as previously described. In the second method we use reagents and materials provided by the QIAamp DNA Blood... 

In this chapter we describe methods used to analyze biological samples by sequencing analysis. The main area focuses on methods used typically in routine laboratories. The applications described are examples that represent the wide field of applications for sequencing in daily analysis work. All methods described here are based on polymerase chain reaction (PCR), which is described in Chapter 2. Generally, the success of analysis depends on the correct sampling and storage, the DNA content of the sample, and the correct DNA extraction method. 

Di Pinto A, Forte VT, Guastadisegni MC, Martino C, Schena FP, TantiUo G (2007). A comparison of DNA extraction methods for food analysis. Food Control, 18 76-80. 

Chapela MJ, Sotelo CG, Perez-Martin RI, et al., (2007a). Comparison of DNA extraction methods from muscle of canned tuna for species identification. Food Control, 18 1211-1215. 

DNA Extraction by Silica Adsorption The silica DNA extraction method is an efficient method able to recover up to 90% of the sample DNA. The extracted DNA has various sizes ranging from 100 bp DNA fragments and up to high-molecular-weight DNA. 

Abriouel, H., Ben Omar, N., Lopez, R. L., Martfnez-Canamero, M., Keleke, S., Galvez, A. (2006). Culture-independent analysis of the microbial composition of the African traditional fermented foods poto poto and degue by using three different DNA extraction methods. International Journal of Food Microbiology, 111, 228-233. 

Here, we discuss an optimized DNA extraction method specifically for electrode-adhered biofilm communities. We describe the use of a biofilm DNA extraction kit from MoBio Laboratories Inc. to extract DNA from electrode-adhered communities (PowerBiofilm DNA Isolation Kit, www.mobio.com). Here, we included a slightly modified alternative for cell lysis to include less meehanical bead-beating and multiple heat treatments. This method for cell lysis has been found to be successful for electrogenic community analyses [18, 21], We do not endorse MoBio Laboratories. This protocol is solely meant to be a starting point for researchers. 

Salonen A, Nikkila J, Jalanka-Tuovinen J, et al. Comparative analysis of fecal DNA extraction methods with phylogenetic microarray effective recovery of bacterial and archaeal DNA using mechanical cell lysis. J Microbiol Methods. 2010 81(2) 127—134. 

Coyle, H. M., et al. "Criminalistics— Technical Notes—a Simple DNA Extraction Method for Marijuana Samples Used in Amplified Fragment Length Polymorphism (AFLP) Analysis." Journal of Forensic Sciences, 48 2003, 343-347. 

Akada R, Murakane T, Nishizawa Y (2000) DNA extraction method for screening yeast clones by PCR. Biotechniques 28 668-670... 

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