Saturated NaCl solution

Thirty minutes after refluxing had stopped, a trace of copper(I) bromide was added to terminate the conversion. The reaction mixture was cautiously poured on to 500 g of finely crushed ice, then 200 ml of 4 N hydrochloric acid were added. After the remaining ice had melted the layers were separated and the aqueous layer was extracted three times with diethyl ether. The combined ethereal solutions were washed with saturated NaCl solution and dried over magnesium sulfate. The greater part of... 

After vigorous shaking the layers were separated and three extractions of the agueous layer with diethyl ether were carried out. The combined solutions were washed twice with saturated NaCl solution and then dried over magnesium sulfate. The greater part of the solvents was distilled off at normal pressure through a... 

Suspend 5 g of soluble starch in 50 mL of saturated NaCl solution, and stir slowly into 500 mL of boiling saturated NaCl solution. Cool and bottle. Free iodine produces a blue-black color. 

A water sample (500-mL) is acidified to pH 3 with HCl and mixed with 20 mL of saturated NaCl solution. The sample is extracted via liquid/liquid partitioning into methylene chloride (3 x 50 mL). The combined methylene chloride fraction is reduced to dryness via rotary evaporation and the residue reconstituted in water-ACN (3 1, v/v). The final determination is performed using HPLC/UV with a two-column switching system. 

Transfer a 500-mL water sample to a 1000-mL separatory funnel, add 20 mL of saturated NaCl solution and adjust the pH to 3 4i 0.1 using either 0.12N HCl or 0.1M NaOH. Partition the water sample with 50 mL of dichloromethane. After phase separation, dry the dichloromethane portion through a pad of sodium sulfate (prewashed with 50 mL of dichloromethane). Repeat the partition procedure two additional times, drying each fraction. Concentrate the pooled dichloromethane fractions... 

Dissolution is based on complex formation Lead sulfide in saturated NaCl solution PbS04 (s) + 4 NaCl (aq) —> Na2[PbCl4] (aq) + Na2SO+ (aq)... 

Chloride salt (la). The pyridinium mesylate from above was dissolved in 200 ml of methylene chloride, and was shaken with 50 ml of saturated NaCl solution in a separatory funnel for ca 2 min. The methylene chloride phase was removed, the brine phase was washed once with 20 ml of methylene chloride, and the organic phases were combined. This procedure was repeated twice using fresh brine. The methylene chloride phase was filtered and evaporated to dryness, affording the crude chloride salt. Recry-stalllzation of the salt from THE/chloroform (ca. 20 1) gives white plates with mp = 192-193 ... 

HFB, the reaction mixture was poured into 25 ml of ethyl ether and 50 ml of distilled water. The aqueous layer was discarded and the organic layer was washed twice with 25-ml portions of 10% aqueous NaOH and with 25 ml of a saturated NaCl solution. The ether solution was dried (MgS04) and concentrated (rotary evaporation) to give a crude product in ca. 90% yield. The crude products were recrystallized usually from ethanol or ethanol H20 to yield primarily the para-disubstitution products. 

The product (3/ ,45)-3-allyl-4-hydroxy-2-pentanone was isolated by extracting the crude reaction mixture with EtOAc (2 x 100 mL). The combined organic layers were then extracted with saturated NaCl solution (70 mL), dried over MgS04 and evaporated to dryness to afford optically active (3/ ,45)-3-allyl-4-hydroxy-2-pentanone... 

NaOH solution (2 m) ethyl acetate (200 mL) saturated NaCl solution (70 mL) anhydrous MgS04 (3 g) pH meter... 

XAD-16 adsorber resin (16 g) (Rohm and Haas France S.A.S., Lauterbourg, France) isopropanol (2 L) ethyl acetate (4.4 L) saturated NaCl solution (800 mL)... 

Tryptaminefrom Tryptophan. JCS, 3993 (1%5). Place 10 g of powdered tryptophan into 500 ml of diphenyl ether and reflux for 60 min under a nitrogen atmosphere. Cool and extract with three 40 ml portions of 2 N HCl acid. Wash the extract with ether, basify with 6 N NaOH and extract with five 50 ml portions of ether. Wash the extract with water and then with a saturated NaCl solution. Dry, evaporate in a vacuum and recrystallize from benzene to get the tryptamine. 

Phenylhydrazine. 10 g of freshly distilled aniline are added to a solution of 30 g coned hydrochloric acid in 75 cc of water and diazotized with 30 cc of water containing 8 g sodium nitrite, with external cooling below 5°. 30 g sodium chloride are added with very fast stirring and cooling of -5°. A solution of 60 g of stannous chloride in 25 g coned hydrochloric acid are then added and allowed to stand. Phenylhydrazine hydrochloride separates after 4-12 hours and is filtered off, washed with a saturated NaCl solution, treated with an excess caustic soda solution, and extracted with ether. The ethereal solution is dried with caustic potash, the ether is removed by evaporation, and the phenylhydrazine is purified by freezing or distilling in vacuo. Tield 10 g 90% theoretical. 

EXTRACTION OF URINE. One cubic centimeter of urine was acidified by addition of two drops of concentrated HC1. After addition of 2 cm3 of saturated NaCl solution, the urine was extracted with 3x3 cm3 of ethyl acetate. Centrifugation was used to help sep-... 

To a solution of (2) (3.11 g, 11 mmol) dissolved in dichloromethane (15 ml) and cooled to -78°C was added under argon cyclopentadiene (1.2 ml, 17 mmol). After standing at -78°C for 36h, NaOH 0.2 N (11 mmol) was added and the mixture stirred for 30 min. Dichloromethane extraction, washing by saturated NaCl solution, drying on MgS04, concentration in vacuo and purification by flash chromatography on silica afforded (Rs)-(+)-(3) as a yellow oil (1.6 g, 62% yield). 

The sorbed heparin fully retains its anticoagulant activity the thrombin time for both sorbed heparin and heparin in solution is 20 + 2 s/mg heparin. The clotting time of blood in contact with the gel is proportional to the amount of heparin sorbed and significantly higher than that characteristic for the non-heparinized gel. Heparin may be repeatedly sorbed after it has been removed with saturated NaCl solution. 

Use a saturated NaCl solution instead of water if working with samples containing a significant amount of short-chain fatty acids, such as milk fat and coconut oil (Bannon et al 1982). 

Add 0.1 ml of glacial acetic acid followed by 5 ml of saturated NaCl solution to the tubes. 

Cholesterol Remove free fatty acids 3. Add 10 ml n-heptane, reflux for another 2 to 3 min, and cool to room temperature. 4. Quantitatively transfer the refluxed solution to a separatory funnel and add 5 ml saturated NaCl solution. 

Spotting device 1-p.l disposable micropipet (e.g., Drummond Microcap), I-pi microsyringe, or (semi)automatic sample spotter Constant humidity chamber containing a cup of saturated NaCl solution 100°C oven... 

Add 10 to 15 ml half-saturated NaCl solution under continued gentle shaking until the hypophase (lower phase), which contains the original organic solvent (e.g., acetone), is -50% aqueous and the chlorophylls and carotenoids are transferred to the organic epiphase. 

The half-saturated NaCl solution prevents emulsion formation. 

Combine the epiphases from the two extractions and wash once or twice with a small amount (e.g., 1 or 2 ml) of half-saturated NaCl solution. 

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