Dip slides

These are microscope slides covered with a thin layer of nutrient agar or selective medium. They are dipped into liquid samples, the excess liquid is shaken off, the slide is transported to the laboratory for incubation and then may be counted under a microscope. They give overnight results and may be used in the field when immediate access to a laboratory is not possible. Due to the variations in the quantity of sample retained on the slide, they only give a rough indication of numbers and should always be supported by alternative counting methods. 

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Place emulsion-coated slide in a horizontal position and wipe the back of the dipped slide with a paper towel or a damp sponge and place the slide flat on a drying rack protected from safelight exposure for 1 h at 45-50% relative humidity (RH). The emulsion will gel in approximately 1 h but can be speeded up by placing the slide on a cold metal plate for 15-... 

Note The dipped slide can be placed vertically for drying but results in an emulsion layer that varies in thickness from top to bottom of the slide and is, therefore, not recommended. 

However, in the same way that it is unwise for water treaters to rely only on metal coupons when assessing corrosion rates, or only on dip-slides when identifying microbiological infections, information obtained from LSI and SI tools should always be used in conjunction with other sources of related and relevant information. Simple predictive indices should not be used as sole criteria for water management action or change, nor an overreliance placed on them. To obtain high cycles of concentration (COC) yet balance the increased risk of deposition, the use of ion-association predictive modeling is preferred to LSI and SI. 

Microbiological monitoring, including various types of dip-slides. Frequently plate count and other related monitoring techniques are carried out in a laboratory. 

Fig. 10.2 Various monitoring devices (1 to r) corrosion/fouling/ biofouling mesh coupons, LPRM monitor, corrosion rack, deposit monitor, membrane filter on poured plate, and dip-slide... 

The most frequently used dip-slide is the total aerobic bacteria/total bacteria count (TAB/TBC). The method employed is to unscrew the cap, remove the dip-slide by the cap, and immerse it into the cooling water sample for 2 to 3 seconds so that both media surfaces are fully covered by liquid. Any excess liquid should be quickly drained and the cap screwed back on the bottle. The bottle should be stored upright for 24 to 36 hours at 25 to 30° C. Then the (usually colored) bacterial colonies should be compared with a chart for conversion to a (reasonably) quantitative interpretation of the results. Typically, the cooling water results will indicate a TAB of between 1 x 103 and 1 x 107 cfu/ml. 

Subsequent physical inspections will tend to reveal the effectiveness of the program. Inspections should be supported by simple diagnostic monitoring tests such as dip-slides. 

Description for any type of bacteria found freely in cooling water rather than bound up in biofilm and most commonly tested by means of dip-slides. A poorly maintained cooling system may contain 1 x 106 or more colony forming units (cfu) per milliliter of water. 

Remove each Autostainer Slide Rack with the slides from the PTLink Tank and immediately dip slides into a jar/tank (PTLink Rinse Station) containing diluted, room temperature Dako Wash Buffer (10x). 

Wash = dip slides in dish of 0.1 M PBS supplemented with 0.2% gelatin (PBSG), then replace with fresh buffer and place on stirplate for 5 min. 

Dip slides briefly in diluted stain, rinse several times with water and mount. 

Dip immediately three times in 70% ethanol to stop the reaction and dip slide several times in water. 

Air-dry, examine and photograph the slide by microscope (oil immersion). Remove the oil by a quick dip in xylene before destaining and ISH. Destain by dipping slides three times for 5 min in 70% ethanol and once in 95% and 100% ethanol, 5 min each, and air-dry. 

Fig. 1. (A) In situ localization of CNTFRa mRNA in coronal section of adult mouse brain. Shown is a low-power dark-field photomicrograph of emulsion-dipped slides of mouse sections hybridized with CNTFRa antisense " S-labeled RNA probe. (B) List of reported distribution of the CNTFRa and method of assessment. Detection of CNTFRa mRNA by Northern Blot, RNase Protection assay or RT-PCR (NRT) or in situ hybridization (ISH). Detection of CNTFRa protein by Western Blot (WB) or immunohistochemistry (IHC). Detection in in vitro primary cell culture (1°).

The main question that has been raised is whether failure of roof support in Spring Quarry is likely to cause trouble in Tunnel Quarry by initiating lateral down-dip roof slide. There seems no reason to anticipate such sliding, for failure in Spring Quarry would not open up any space to receive down-dip slide of roof. Careful examination has, moreover, not revealed any tendency to lateral... 

Snbbed slides Dissolve gelatin (1 g) in 1 L of hot distilled water. Cool and add 0.1 g chrominm potassium sulfate. Store in refrigerator. Dip slides two to three times in the solntion. Drain and dry in a vertical position. Store in a dust-free box. 

Dip slides in emulsion slowly (a few seconds down, a few seconds up) and remove emulsion from the back of the slide with Kim-wipes. Slides are always boxed in the same orientation to avoid inadvertently touching the sections. 

The membrane filter process and the dip slide process are unofficial methods of determining the colony count. 

The dip slide process is based on the membrane filter method. It uses commercially available test kits, comprising a nutrient medium carrier in which the medium part is inserted in a beaker. This beaker serves as dipper for taking the water sample and also protects the nutrient medium part from secondary infection and from drying out during the incubation period. 

Dip slides briefly into the DEPC water, then place in the acetylation bath for 10 min with stirring. (A small stir bar or flea fits beneath standard slide racks and allows... 

Dip slides briefly and with a uniform speed into the emulsion, wipe the back (nonsection side) of each, and lay section side up on cold tray for lOmin to set. If bubbles or streaks are visible, then redip the section. 

Study on such landslides whose slip direction deviates significantly from the strata tendency is summarized as apparent dip sliding by many scholars " , so that some common features of... 

YIN Yue-ping. Mechanism of apparent dip slide of inclined bedding rockslide-a case study of Jiweishan... 

Dipping slides to coat them. slides properly. Second, the slurry simply may... 

Screw-cap jar filled with slurry (capped and shaken vigorously before dipping slides)... 

Standard biomedical science methods of plating-ont, incnbating and plate counting are used for dip-slides. 

Fuel samples can be tested with these dip slides by first emulsifying the fuel and then sampling the emulsion with the dip slide. With the AFNOR Test NFM 07-070 1993, fuel emulsified in water (1 10) is tested for growth with a dipstick. This test detects only bacterial growth > 10 bacteria/L (Institute of Petroleum, 1996). 

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