Derivatives, high-performance liquid

Minguillon, C., Franco, P., Oliveros, L., Lopez, P. Bonded cellulose-derived high-performance liquid chromatography chiral stationary phases. I. Influence of the degree of fixation on selectivity,... 

Canjura, F.L. and Schwartz, S.J., Separation of chlorophyll compounds and then-polar derivatives by high-performance liquid chromatography, J. Agric. Food Ghem., 39, 1102, 1991. 

For the high-performance liquid chromatography (HPLC) determination of napro-anilide and its metabolite, 200 mL of 2% sodium sulfate in 0.1M potassium hydroxide solution are added to the concentrate derived from Section 2.2.2. The solution is shaken twice with 100 mL each of dichloromethane or ethyl acetate-n-hexane (1 1, v/v) for 10 min. The combined organic layer is concentrated. " ... 

Inject an aliquot (Vi) of the solution derived from Section 6.2.2 (Vend) for soils into the high-performance liquid chromatograph. 

Milbemectin consists of two active ingredients, M.A3 and M.A4. Milbemectin is extracted from plant materials and soils with methanol-water (7 3, v/v). After centrifugation, the extracts obtained are diluted to volume with the extraction solvent in a volumetric flask. Aliquots of the extracts are transferred on to a previously conditioned Cl8 solid-phase extraction (SPE) column. Milbemectin is eluted with methanol after washing the column with aqueous methanol. The eluate is evaporated to dryness and the residual milbemectin is converted to fluorescent anhydride derivatives after treatment with trifluoroacetic anhydride in 0.5 M triethylamine in benzene solution. The anhydride derivatives of M.A3 and M.A4 possess fluorescent sensitivity. The derivatized samples are dissolved in methanol and injected into a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector for quantitative determination. 

Dominguez, L. M. and Dunn, R. S., Analysis of OPA-derived amino sugars in tobacco by high-performance liquid chromatography with fluorimetric detection, /. Chromatogr. Sci., 25, 468, 1987. 

Zhao, Q., Sannier, F., Garreau, I., Lecoeur, C., and Piot, J. M., Reversed-phase high-performance liquid chromatography coupled with second-order derivative spectroscopy for the quantitation of aromatic amino acids in peptides application to hemorphins, /. Chromatogr. A, 723, 35, 1996. 

Herraiz, T., Sample preparation and reversed phase-high performance liquid chromatography analysis of food-derived peptides, Analytica Chimica Acta, 352, 119, 1997. 

Vasanits, A. and Molnar-Perl, I., Temperature, eluent flow-rate and column effects on the retention and quantitation properties of phenylthiocarbamyl derivatives of amino acids in reversed-phase high-performance liquid chromatography, J. Chromatogr., A, 832,109, 1999. 

Yang, Y.-B., Harrison, K., and Kindsvater, J., Characterization of a novel stationary phase derived from a hydrophilic polystyrene-based resin for protein cation-exchange high-performance liquid chromatography, /. Chromatogr. A, 723, 1, 1996. 

Rissler, K. (1996). High performance liquid chromatography and detection of polyethers and their mono (carboxy) alkyl and -arylalkyl substituted derivatives. J. Chromatogr. A 742, 1-54. 

The above chemistry has been applied to the synthesis of a series of derivatives which show activity against animal parasites. In order to confirm further the stmcture and configuration of the most active enantiomer of one of these compounds, the enantiomers were separated by chiral high-performance liquid chromatography (HPLC), and single crystal X-ray diffraction of a 2 1 CuCl2 complex was carried out <2005BML2375>. 

Plass, M., Valko, K., Abraham, M. H., Determination of solute descriptors of tripeptide derivatives based on high-throughput gradient high-performance liquid chromatography retention data, J. Chromatogr. A. 1998, 803(1-2), 51-60. 

Erk and Altun [24] used a ratio spectra derivative spectrophotometric method and a high performance liquid chromatographic method for the analysis of miconazole nitrate and metronidazole in ovules. The spectral method depends on ratio spectra first derivative spectrophotometry, by utilizing the linear relationship between substances concentration and ratio spectra first derivative peak amplitude. The ratio... 

Guillaume et al. [69] presented a high performance liquid chromatographic method for an association study of miconazole and other imidazole derivatives in surfactant micellar using a hydrophilic reagent, Montanox DF 80. The thermodynamic results obtained showed that imidazole association in the surfactant micelles was effective over a concentration of surfactant equal to 0.4 pM. In addition, an enthalpy-entropy compensation study revealed that the type of interaction between the solute and the RP-18 stationary phase was independent of the molecular structure. The thermodynamic variations observed were considered the result of equilibrium displacement between the solute and free ethanol (respectively free surfactant) and its clusters (respective to micelles) created in the mobile phase. 

In another study, the authors reported a comparative study of the enantiomeric resolution of miconazole and the other two chiral drugs by high performance liquid chromatography on various cellulose chiral columns in the normal phase mode [79], The chiral resolution of the three drugs on the columns containing different cellulose derivatives namely Chiralcel OD, OJ, OB, OK, OC, and OE in normal phase mode was described. The mobile phase used was hexane-isopropanol-diethylamine (425 74 1). The flow rates of the mobile phase used were 0.5, 1, and 1.5 mL/min. The values of the separation factor (a) of the resolved enantiomers of econazole, miconazole, and sulconazole on chiral phases were ranged from 1.07 to 2.5 while the values of resolution factors (Rs) varied from 0.17 to 3.9. The chiral recognition mechanisms between the analytes and the chiral selectors are discussed. 

Clark et al. [81] determined the time course of A-acetylation of primaquine by Streptomyces roseochromogenous and Streptomyces rimosus by quantitative high performance liquid chromatographic analyses of the culture broths. The A-5-bistri-fluoroacetyl derivative of primaquine was used as an internal standard in the analysis for the quantitation of primaquine A-acetate in microbial culture broths. S. roseochromogenous forms the highest level of primaquine A-acetate at 24—36 h after substrate addition, while S. rimosus is slower in its acetylation, peaking at 3 days after substrate addition. The formation of a novel dimeric compound from the reaction of primaquine with 8-(4-phthalimido-l-methylbutylamino)-6-methoxy quinoline is also reported. 

Okamoto et al [85] performed the optical resolution of primaquine and other racemic drugs by high performance liquid chromatography using cellulose and amylose tris-(phenylcarbamate) derivatives as chiral stationary phases. Primaquine and other compounds were effectively resolved by cellulose and/or amylose derivatives having substituents such as methyl, tertiary butyl, or halogen, on the phenyl groups. 

A.N. Assimopoulou, I. Karapanagiotis, A. Vasiliou, S. Kokkini and V.P. Papageorgiou, Analysis of alkannin derivatives from Alkanna species by high performance liquid chromatography/ photodiode array/mass spectrometry, Biomed. Chromatogr., 20, 1359 1374 (2006). 

Hamisch, M., Mockel, H.J., Schule, G. (1983) Relationship between log Pow shake-flask values and capacity factors derived from reversed-phase high-performance liquid chromatography for n-alkylbenzenes and some OECD reference substances. J. Chromatogr. 282, 315-332. 

Berardini N, Carle R and Schieber A. 2004. Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. Tommy Atkins ) peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 18(19) 2208—2216. 

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