Depolymerases

Each fruit has specific quantities and ratio of pectin, hemicelluloses and cellulose. These polysaccharides are important concerning enzymes activities required to produce juices and concentrates. Moreover, even if molecular weight and methylation degree of the pectin are specific for each fruit, during the fruit maturation, endogenous pectinases depolymerases and esterase are changing the pectin characteristics This broad variability of raw material makes difficult the standardisation of fruits processing. [Pg.453]

Bertheau, Y., Madgidi-Hervan, E., Kotoujansky, A., NguyenThe, C., Andro, T. and Coleno, A. 1984. Detection of depolymerase isoenzymes after electroporesis or electrofocusing... [Pg.880]

R. Verger, in Hydrolases et Depolymerases. Enzymes d Interet Industriel Gauthier-Villars, Paris, 1985, pp. 313-329. [Pg.584]

Practically nothing is known about how PHA synthases and intracellular PHA depolymerases, which are both bound to the granules, interact and whether or... [Pg.117]

Fig. 1. The metabolic cycle for the synthesis and degradation of poly(3HB). (1) 3-ketothiolase (2) NADPH-dependent acetoacetyl-CoA reductase (3) poly(3HB) synthase (4) NADH-dependent acetoacetyl-CoA reductase (5), (6) enolases (7) depolymerase (8) d-(-)-3-hydroxybutyrate dehydrogenase (9) acetoacetyl-CoA synthetase (10) succinyl-CoA transferase (11) citrate synthase (12) see Sect. 3...

The lack of intracellular depolymerases which degrade the accumulated PHAs... [Pg.185]

Synthesis of PHAs in plants can not only be used directly in biotechnology for the creation of novel crop plants, but can also be a utilized as a unique novel tool in the basic studies of plant biochemistry. PHA synthesized in plants acts as a terminal carbon sink, since plants do not have enzymes, such as PHA depolymerases [68], required for degradation of the polymer. The quantity and composition of PHA can thus be used to monitor the quantity and quality of the carbon flux to different pathways. [Pg.222]

Polyesters, such as microbially produced poly[(P)-3-hydroxybutyric acid] [poly(3HB)], other poly[(P)-hydroxyalkanoic acids] [poly(HA)] and related biosynthetic or chemosynthetic polyesters are a class of polymers that have potential applications as thermoplastic elastomers. In contrast to poly(ethylene) and similar polymers with saturated, non-functionalized carbon backbones, poly(HA) can be biodegraded to water, methane, and/or carbon dioxide. This review provides an overview of the microbiology, biochemistry and molecular biology of poly(HA) biodegradation. In particular, the properties of extracellular and intracellular poly(HA) hydrolyzing enzymes [poly(HA) depolymerases] are described. [Pg.289]

The poly(HA) depolymerases of the bacteria Alcaligenes faecalis (strains AE122 and Tl), Comamonas acidovorans, Comamonas testosteroni, Comamonas sp., Pseudomonas fluorescens, Pseudomonas lemoignei, Pseudomonas stutzeri, Ralstonia pickettii, Streptomyces exfoliatus, and of the fungi Paecilomyces lilaci-nus, Penicillium funiculosum, and Penicillium pinophilum have been purified and characterized (for details see Table 1). Poly(HA) depolymerases share several characteristics ... [Pg.293]

A relatively small molecular weight (<70 kDa) with most depolymerases consisting of only one polypeptide. [Pg.293]

Most poly(HA) depolymerases are inhibited by reducing agents, e.g., dithio-erythritol (DTT), which indicates the presence of essential disulfide bonds, and by serine hydrolase inhibitors such as diisopropyl-fluoryl phosphate (DFP) or acylsulfonyl derivates. The latter compounds covalently bind to the active site serine of serine hydrolases and irreversibly inhibit enzyme activity [48]. [Pg.293]

Table 1. Overview on poly(HA)-degrading microorganisms and biochemical characterization of purified poly(HA)-depolymerases... [Pg.294]

Isolate from seawater, depolymerase with unusual high apparent Mr 183... [Pg.295]

Depolymerase with the highest temperature opt., however unstable at 70°C 186... [Pg.295]

Poly(HA)SCL depolymerase with high poly(3HV) depolymerase activity... [Pg.295]

Relationship between poly(3HB) depolymerase synthesis and uptake of succinate 88 Depolymerase with acidic pH optimum 183... [Pg.295]

Po HAscl) depolymerases of Gram-negative aerobic bacteria continued ... [Pg.296]

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