Poly depolymerase, properties

Polyesters, such as microbially produced poly[(P)-3-hydroxybutyric acid] [poly(3HB)], other poly[(P)-hydroxyalkanoic acids] [poly(HA)] and related biosynthetic or chemosynthetic polyesters are a class of polymers that have potential applications as thermoplastic elastomers. In contrast to poly(ethylene) and similar polymers with saturated, non-functionalized carbon backbones, poly(HA) can be biodegraded to water, methane, and/or carbon dioxide. This review provides an overview of the microbiology, biochemistry and molecular biology of poly(HA) biodegradation. In particular, the properties of extracellular and intracellular poly(HA) hydrolyzing enzymes [poly(HA) depolymerases] are described. [Pg.289]

The poly(3HO) depolymerase differs from poly(HASCL) depolymerases in several of its biochemical properties it is specific for poly(HAMCL) and for artificial esters such as p-nitrophenylacyl esters with six or more carbon atoms in the fatty acid moiety. Poly(3HB) and other poly(HASCL) are not hydrolyzed. The enzyme is not inhibited by dithioerythritol or by EDTA and therefore apparently does not contain essential disulfide bonds. It is also not dependent on Ca2+ or other divalent cations. [Pg.306]

Although there is evidence that all poly(HA) depolymerases cleave the polyesters by the same mechanism (catalytic triad), the poly(3HO) depolymerase differs considerably from poly(HASCL) depolymerases in terms of primary sequence and polymer-binding. This might be due to different approaches of these enzymes to get access to the polymers reflecting the distinctive physicochemical properties of poly(HASCL) and poly(HMCLA) rather than coevolution. [Pg.306]

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Abe T, Kobayashi T, Seiito T (2005) Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutiopha H16. J Bacteriol 187 6982-6990... [Pg.314]

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