Comamonas acidovorans

Groenewegen PEG, P Breenwer, JMLM van Helvoort, AAM Langenhoff, EP de Vries, JAM de Bont (1992) Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10. J Gen Microbiol 138 1599-1605. 

Akutsu Y, T Nakajima-Kambe, N Nomura, T Nakahara (1998) Purification and properties of a polyester poly-urethane-degrading enzyme from Comamonas acidovorans TB-35. Appl Environ Microbiol 64 62-67. 

The poly(HA) depolymerases of the bacteria Alcaligenes faecalis (strains AE122 and Tl), Comamonas acidovorans, Comamonas testosteroni, Comamonas sp., Pseudomonas fluorescens, Pseudomonas lemoignei, Pseudomonas stutzeri, Ralstonia pickettii, Streptomyces exfoliatus, and of the fungi Paecilomyces lilaci-nus, Penicillium funiculosum, and Penicillium pinophilum have been purified and characterized (for details see Table 1). Poly(HA) depolymerases share several characteristics ... 

Miehthling, R. Hecht, V., and Deckwer, W. D., Microbial Degradation of Quinoline, Kinetic Studies with Comamonas Acidovorans DSM 6426. Biotech. Bioeng, 1993. 42 pp. 589-95. 

Zeth, K., Diederichs, K., Welte, W., and Engelhardt, H. (2000). Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolution. Structures, 981-992. 

Allen, A. B., Hilliard, N. P. Howard, G. T. (1999). Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans. International Biodeterioration and Biodegradation, 43, 37-41. 

Nakajima-Kambe, T., Onuma, F., Akutsu, Y. Nakahara, T. (1995). Determination of the polyester polyurethane breakdown products and distribution of the polyurethane degrading enzyme of Comamonas acidovorans strain TB-35. Journal of Fermentation and Bioengineering, 83, 456-60. 

Comamonas testosteroni Comamonas acidovorans Ralstonia pickettii... 

In addition to the more common (S)-amidase activities, (RJ-specific enzymes have also been identified. Thus (S)-ketoprofenamide has been derived from the racemic mixture using a biocatalyst from Comamonas acidovorans KPO 2771-4 to hydrolyze the (R)-enantiomer with high selectivity1141 (see Scheme 12.2-6). 

Asano et al. have also purified a D-stereospecific amino acid amidase from another Ochrobadrum anthropi isolate137, 38i. Recently, a new amidase from Comamonas acidovorans has been reported that exhibits a broad substrate specificity and also d-amino acid amidase activity1391. In addition, a D-specific amidase has been identified in Arthrobacter sp. NJ-261401. In contrast to the D-selective enzymes of Ochrobadrum sp. and Comomonas acidovorans, the D-amide hydrolase identified in Arthrobacter sp. NJ-26 was very substrate specific a good hydrolysis rate was only observed for d-alanine amide. 

The data base PORINS consisted of seven porins and two defensins all with known or proposed transmembrane P-strand structure. The porins with known X-ray structure were porin from Rhodobacter capsulatus [10,28] and porins PhoE and OmpF from Escherichia coli [39,11]. Porins with proposed transmembrane P-barrel topology were anion-selective porin Omp32 from Comamonas acidovorans [40], outer membrane protein OmpA from Escherichia coli K12 membrane (membrane-embedded fragment residues 1 to 177, [41,42]), and mitochondrial outer membrane porin from human B-lymphocytes [43] and from Neurospora crassa [44]. Two defensins of known structure were HNP-3 [45] and defensin from larvae of the dragonfly Aeschna cyanea [46]. 

Alcaligenes latus [8,12], Comamonas acidovorans [9,13], Comamonas testostero-nii [14] and Hydrogenphaga pseudoflava [15] have been reported. In the present study, we report the biosynthesis of P(3HB-co HB) by Cupriavidus sp. USMAA1020 that was isolated from Malaysian environment... 

Lee W-H, Azizan MNM, Sudesh K (2004) Effects of culture conditions on the composition of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Comamonas acidovorans. Polym Degrad Stab 84 129-134... 

Mitomo H, Hsieh WC, Nishiwaki K, Kasuya K, Doi Y (2001) Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) produced by Comamonas acidovorans. Polymer 42 3455-3461... 

Recently, the degradation of 4-nitrobenzoate by a Comamonas acidovorans NBA-10 was shown to proceed via reduction of the nitro group, but without the formation of 4-aminobenzoate. Cell extracts, in presence of NADPH, degraded 4-nitrobenzoate into 4-hydroxylamino-... 

Figure 6. Proposed degradative pathway of 4-NBA by Comamonas acidovorans NBA-10 [81].

PBS Alcaligenes faecalis, Pseudomonas stutzeri, Comamonas acidovorans Kasuya et al. (1999) Packaging materials, dishware, fibers, agricultural film materials, medical materials Liu et al. (2009), Bhatia et al. (2007), Lee and Wang (2006)... 

Lee HJ, Chd MH, Kim TU, Yoon SC (2001) Accumulation of polyhydroxyalkanoic acid containing large amounts of unsaturated monomers in Pseudomonas fluorescens BM07 utilizing saccharides and its inhibition by 2-bromooctanoic acid. Appl Environ Microbiol 67 4963-4974 Lee WH, Azizan MNM, Sudesh K (2004) Effects of culture conditions on the composition of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Comamonas acidovorans. Polym Degrad Stab 84 129-134... 

Saito Y, Doi Y (1994) Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans. Int J Biol Macromol 16 99-104 Saito Y, Nakamura S, Hiramitsu M, Doi Y (1996) Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Polymer Int 39 167-174 Salehizadeh H, Van Loosdrecht MCM (2004) Production of polyhydroxyalkanoates by mixed culture recent trends and biotechnological importance. Biotechnol Adv 22 261-279... 

Iwata T, Doi Y, Tanaka T, Akehata T, Shiromo M, Teramachi S (1997b) Enzymatic degradation and adsorption on poly[(/J)-3-hydroxybutyrate] single crystals with two types of extracellular PHB depolymerases from Comamonas acidovorans YM1609 and Alcaligenes faecalis Tl. Macromolecules 30 5290-5296... 

Alcaligenes eutrophus Alcaligenes latus Comamonas acidovorans 4-hydrox) but)Tic acid y-but)Tolactone 1,4-butanediol 1,6-hexanediol ( V 7 0 CH) CH, (RV3HB 4HB 0... 

A random copolyester of 3HB and 4-hydroxybutyric acid, P(3HB-co-4HB), was produced by A. eutrophus (Kunioka et al., 1988, 1989 Nakamura et al., 1992), A. latus (Hiramitsu et al., 1993) or Comamonas acidovorans (Saito and Doi, 1994), when dr-hydroxybutyric acid, l,4r-butanediol or y-butyrolactone was used as the carbon source. The P(3HB-co-4HB) copolymers with a wide range of compositions from 0 to 100 mol% 4HB was produced by A. eutrophus bora the mixed carbon substrates of 3-hydroxybutyric and 4-hydroxybutyric acids in the presence of some additives (Nakamura et al., 1992). When drhydroxybutyric acid, citrate, and ammonium sulfate were fed to A. eutrophus, P(3HB-co-4HB) copolymers with compositions of 70-100 mol% 4HB were produced. In contrast, C. acidovorans produced a P(4HB) homopolymer in the presence of 1,4-butanediol or d-hydroxybutyric acid without additives (Saito and Doi, 1994). 

Saito, Y. and Doi, Y. (1994) Microbial synthesis and properties of poly(3-hydrox) butyrate-co4-hydroxy-butyrate) in Comamonas acidovorans, Int.J. Biol. Maaomol. 16, 99-104. 

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