Assay comet

coli Fpg protein (for- mamidopyrimidine-DNA glycosylase) Oxidized purines FAPY-A, FAPY-G, 8-oxo-G, 8-oxo-A, some abasic sites, to a lesser extent other modified purines [Pg.493]

coli Nth protein (endonuclease III) Oxidized pyrimidines Ring-saturated or fragmented Thy residues, e.g. F Thy, Tg, 5-hydroxy-5-methylhydantoine, urea, DNA damaged at Gua, some abasic sites [Pg.493]

coli Nfo protein (endonuclease IV) AP sites Several AP sites including oxidized AP sites, urea [Pg.493]

Gasiorowski, K., Brocos, B. DNA repair of hydrogen peroxide-induced damage in human lymphocytes in the presence of four antimutagens. A study with alkaline single cell gel electrophoresis (comet assay). Cellular Molecular Biology Letters, Vol.6, (2001), pp. 897-911, ISSN 1425-8153... [Pg.198]

Lebailly P, Vigreux C, Lechevrel C, et al. 1998. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay Modifications of DNA damage levels after a one-day field spraying period with selected pesticides. Cancer Epidemiol Biomarkers Prev 7 929-940. [Pg.303]

Sasaki, Y.F. et al., The comet assay with 8 mouse organs results with 39 currently used food additives, Mutation Res., 519, 103, 2002. [Pg.99]

Collins, A. R. (2004). The COMET assay for DNA damage and repair. Molecular... [Pg.59]

Betti, C. and M. Nigro. 1996. The Comet assay for the evaluation of the genetic hazard of pollutants in cetaceans preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro. Mar. Pollut. Bull. 32 545-548. [Pg.425]

Jackman, S.M., et al., DNA damage assessment by comet assay of human lymphocytes exposed to jet propulsion fuels, Environ, Mol. Mutagen., 40, 18, 2002. [Pg.236]

In Vivo (or In Vitro) Single-Cell Gel Electrophoresis Test for DNA Damage (Comet Assay)... [Pg.308]

Olive, P.L., Banath, J.P., and Durand, R.E., Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay, Radiat. Res., 122, 86, 1990. [Pg.313]

Hartamann, A., Schumacher, M., Plappert-Helbig, U., Lowe, P., Suter, W., and Mueller, L., Use of the alkaline in vivo comet assay for mechanistic genotoxicity investigation, Mutagenesis, 19, 51, 2004. [Pg.313]

Sasaki, Y.F., Kawaguchi, S., Kamaya, A., Ohshita, M., Kabasawa, K., Iwama, K., Taniguchi, K., and Tsuda, S., The comet assay with 8 mouse organs results with 39 currently used food additive, Mutat. Res., 519,103, 2002. [Pg.313]

Sekihashi, K., Yamamoto, A., Matsummura, Y., Ueno, S., Watanabe-Akanuma, M., Kassie, F., Knasmuller, S., Tsuda, S., and Sasaki, Y.F., Comparative investigation of multiple organs of mice and rats in the comet assay, Mutat. Res., 517, 53, 2002. [Pg.313]

Biscardi, D., Monarca, S., De Fusco, R., Senatore, F., Poli, P., Buschini, A., Rossi, C. and Zani, C. (2003). Evaluation of the migration of mutagens/carcinogens from PET bottles into mineral water by 7>a<7eicawtw/micronuclei test. Comet assay on leukocytes and GC/MS, Sci. Total Environ., 302, 101-108. [Pg.330]

M. Venturi, R. J. Hambly, B. Glinghammar, J. J. Rafter and I. R. Rowland, Genotoxic activity in human faecal water and the role of bile acids a study using the alkaline comet assay. Carcinogenesis, 1997,18(12), 2353. [Pg.61]

Utilising the alkaline comet assay Jolly et al showed that exposing the nontumourigenic squamous oesophageal cell line, Het-IA to deoxycholate at... [Pg.75]

Figure 4.1 The comet assay. A single-cell suspension is embedded in agarose on a slide. Cells are then subject to lysis followed by electrophoresis. If present, damaged DNA migrates out of the nucleoid structure during electrophoresis to producing a characteristic comet shape. Double-strand breaks are revealed under neutral conditions, whereas alkali conditions additionally show single-strand breaks and alkali labile sites. Image analysis of stained DNA is used to quantitate the amount of damaged DNA in the comet tail.

Duty SM, Singh NP, Silva MJ, Barr DB, Brock JW, Ryan L, Herrick RE, Christiani DC, Hauser R (2003) The relationship between environmental exposures to phthalates and DNA damage in human sperm using the neutral comet assay. Environ Health Perspect 111 1164-1169... [Pg.329]

Kleinsasser NH, Harreus UA, Kastenbauer ER, Wallner BC, Sassen AW, Staudenmaier R, Rettenmeier AW (2004) Mono(2-ethylhexyl) phthalate exhibits genotoxic effects in human lymphocytes and mucosal cells of the upper aerodigestive tract in the comet assay. Toxicol Lett 148 83-90... [Pg.334]

To this end, the authors perform a second comet assay. Only limited results, however, are shared PM2 5 from only one city (Baton Rouge) was tested using only one cell line (K562 myeloid leukemia cells), thereby following a broad-to-narrow approach. The results are presented in excerpt 4E. As shown in Eigure 3 (excerpt 4E), cells were exposed to (A) a blank extract, (B) a PM2 5 extract, (C, D, E) a PM2 5 extract mixed with one of three different free radical scavengers, (E) a positive control, and (G, El) a PM2 5 extract mixed with one of two different metal-ion chelators. The free radical scavengers remove free radicals the... [Pg.133]

Figures. Comet assay is shown for human K562 cells exposed to an extract produced from particulate matter released from sample PM 5 filters, to an extract derived from an unloaded filter, or to hydrogen peroxide (100 pM) as a control. Cells with little or no DNA damage are labeled 1 and 2, and those with extensive damage are labeled 3 and 4.

Table2. Results of Comet Assays for K362 Cells Treated with Baton Rouge... [Pg.137]

Reexamine the drawing of instrumentation, the chromatograms of GC data, and the photographs of comet assays in excerpts 3S, 4C, and 4E, respectively. Which guidelines, if any, in Table 16.1 offer insights on the formatting of such figures ... [Pg.533]

Analysis of Deoxyribonucleic Acid Damage Using the Comet Assay Two... [Pg.604]

DAB was genotoxic in the comet assay inducing DNA damage in the stomach, colon liver, bladder, lung, and bone marrow. It is also mutagenic to Salmonella in the Ames test. Because of its demonstrated carcinogenicity in animals, human exposure to DAB by any route should be avoided. In recent years, this compound has been used only in laboratories as a model of tumorigenic activity in animals. It is not produced commercially in the United States and is of little occupational health importance. [Pg.262]

Tsuda S, Matsusaka N, Madarame H, et al The comet assay in eight mouse organs results with 24 azo compounds. MutatRes 465 11-26, 2000... [Pg.263]

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