In vitro comet assay

Chromosomal aberrations, human lymphocytes in vitro Chromosomal aberrations, human lymphocytes in vitro Aneuploidy, human fetal lung cells in vitro Comet assay on human blood in vitro... [Pg.109]

In Vitro Comet Assay in 3D Human Reconstructed Skin Models... [Pg.318]

More recently, Poul et al (2004) tested iodate alongside bromate in two different in vitro tests - the alkaline Comet assay and the cytokinesis-block micronucleus assay. Iodate concentrations of up to lOmM failed to induce primary DNA damage as determined in the Comet assay, while bromate significantly increased damage at concentrations of 2.5 mM and above. The results of the Comet assay could be confirmed by those obtained in the second test system, which detects not only clastogenic events, but also... [Pg.914]

Betti, C. and M. Nigro. 1996. The Comet assay for the evaluation of the genetic hazard of pollutants in cetaceans preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro. Mar. Pollut. Bull. 32 545-548. [Pg.425]

In Vivo (or In Vitro) Single-Cell Gel Electrophoresis Test for DNA Damage (Comet Assay)... [Pg.308]

Jaloszynski P, Kujawski M, Wasowicz M, et al Genotoxicity of inhalation anesthetics halothane and isoflurane in human lymphocytes studied in vitro using the comet assay. Mutat Res-. 439(2) 199-206, 1999... [Pg.365]

Witte, I., Plappert, U de Wall, H. and Hartmann, A. (2007) Genetic toxicity assessment Employing the best science for human safety evaluation part III The comet assay as an alternative to in vitro clastogenicity tests for early drug candidate selection. Toxicological Sciences, 97, 21-26. [Pg.269]

H., Miyamae, Y, Rojas, E. et al. (2000) Single cell gel/comet assay guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35, 206-221. [Pg.493]

Struwe, M., Greulich, KO., Suter, W. and Plappert-Helhig, U. (2007) The photo comet assay—a fast screening assay for the determination of photogenotoxicity in vitro. Mutation Research, 632, 44—57. [Pg.493]

DNA strand breaks (Comet assay), MCL-5 cells Gene mutation, human TK6 cells in vitro Gene mutation, human AHH-1 cells in vitro Sister chromatid exchange, human lymphocytes in vitro... [Pg.297]

Zeljezic D, Garaj-Vrhovac V, Perkovic P. Evaluation of DNA damage induced by atrazine and atrazine-based herbicide in human lymphocytes in vitro using a comet and DNA diffusion assay. Toxicol in Vitro 2006 20(6) 923—35. [Pg.227]

Moretti M, Villarini M, SimonucciS, et al. Effects of co-exposure to extremely low frequency (ELF) magnetic fields and benzene of benzene metabolites determined in vitro by the alkaline comet assay. Toxicol Lett 2005 157(2) 119 28. [Pg.258]

The ReProComet assay (repair-proficient comet assay) was developed to detect chemically induced DNA damage in sperm cells. In order to overcome the intrinsic DNA repair deficiency of the sperm cells this modified comet assay is based on the addition of a protein extract from HeLa cells to agarose-embedded sperm on microscopic slides previously exposed to mutagenic compounds. A clear-cut dose-dependent effect was measured after addition of the cell extract representing a proof of concept of a novel in vitro mutagenicity test on sperm [18]. Transferability to other laboratories remains to be addressed. [Pg.275]

Kamer, I. and B. Rinkevich. In vitro application of the comet assay for aquatic genotoxicity considering a primary culture versus a cell line. Toxicol. In Vitro 16 177-184, 2002. [Pg.79]

Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantation of low levels of DNA damage in individual ceUs. Exp Cell Res 175 184-91 CoUins AR, Dusinska M, Horvathova E,Munro E,Savio M,StetinaR (2001) Inter-individual differences in repair of DNA base oxidation, measured in vitro with the comet assay. Mutagenesis 16 297-301... [Pg.174]

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