Genotoxicity comet assay

Figure 19.7 Experimental procedure forthe assessment of photocytotoxicity (MTT assay) and photo-genotoxicity (comet assay) on reconstructed epidermis. Drugsorformulationscan be applied on the skin surface (topical route) or provided in the culture medium (systemic route see [76]).

Betti, C. and M. Nigro. 1996. The Comet assay for the evaluation of the genetic hazard of pollutants in cetaceans preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro. Mar. Pollut. Bull. 32 545-548. 

Hartamann, A., Schumacher, M., Plappert-Helbig, U., Lowe, P., Suter, W., and Mueller, L., Use of the alkaline in vivo comet assay for mechanistic genotoxicity investigation, Mutagenesis, 19, 51, 2004. 

M. Venturi, R. J. Hambly, B. Glinghammar, J. J. Rafter and I. R. Rowland, Genotoxic activity in human faecal water and the role of bile acids a study using the alkaline comet assay. Carcinogenesis, 1997,18(12), 2353. 

Kleinsasser NH, Harreus UA, Kastenbauer ER, Wallner BC, Sassen AW, Staudenmaier R, Rettenmeier AW (2004) Mono(2-ethylhexyl) phthalate exhibits genotoxic effects in human lymphocytes and mucosal cells of the upper aerodigestive tract in the comet assay. Toxicol Lett 148 83-90... 

DAB was genotoxic in the comet assay inducing DNA damage in the stomach, colon liver, bladder, lung, and bone marrow. It is also mutagenic to Salmonella in the Ames test. Because of its demonstrated carcinogenicity in animals, human exposure to DAB by any route should be avoided. In recent years, this compound has been used only in laboratories as a model of tumorigenic activity in animals. It is not produced commercially in the United States and is of little occupational health importance. 

Jaloszynski P, Kujawski M, Wasowicz M, et al Genotoxicity of inhalation anesthetics halothane and isoflurane in human lymphocytes studied in vitro using the comet assay. Mutat Res-. 439(2) 199-206, 1999... 

Ralph S, Petras M. 1998. Caged amphibian tadpoles and in situ genotoxicity monitoring of aquatic environments with the alkaline single cell gel electrophoresis (comet) assay. Mutat Res 413 235-250. 

Lemiere, S. (2004). Interest of the Comet Assay for the study of environmental genotoxicity. Thesis, University of Metz. 

Hartmann A, Schumacher M, Plappert-Helbig U, et al. Use of the alkaline in vivo Comet assay for mechanistic genotoxicity investigations. Mutagenesis. 2004 19(1) 51-59. 

Danadevi K, Rozati R, Saleha Banu B, and Grover P (2004) In vivo genotoxic effect of nickel chloride in mice leukocytes using comet assay. Food and Chemical Toxicology 42 751-757. 

Salagovic, J., Gilles, J., Verschaeve, L. and Kalina, I. (1996) The comet assay for the detection of genotoxic damage in the earthworms a promising tool for assessing the biological hazards of polluted sites. Folia Biol., 42, 17-21. 

Comet assay 3D human reconstructed skin models DNA damage in cells or tissues Broad spectrum of damage Genotoxicity hazard identification... 

Kamer, I. and B. Rinkevich. In vitro application of the comet assay for aquatic genotoxicity considering a primary culture versus a cell line. Toxicol. In Vitro 16 177-184, 2002. 

Kammann, U., M. Bunke, H. Steinhart and N. Theobald. A permanent fish cell line (EPC) for genotoxicity testing of marine sediments with the comet assay. Mutat. Res. — Genet. Toxicol. Environ. Mutagen. 498 67-77, 2001. 

Merk, O., and Speit, G. (1999). Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ Mol Mutagen 33, 167-172. 

Speit, G., and Hartmann, A. (2005). The comet assay A sensitive genotoxicity test for the detection of DNA damage. Methods Mol Biol 291, 85-95. 

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