Polyethyleneimine cellulose

The fractionation of simple oligonucleotide mixtures on thin layers of PEl-cellulose (polyethyleneimine - cellulose) has been described by Randerath and Randerath (1967a, b) (see also 2.2.3). For onedimensional fractionation up to 10 mg of oligonucleotides may be... 

Analytical Techniques and Physical Methods.— The mapping of oligonucleotides and nucleic acid digests on cellulose or cellulose-polyethyleneimine has been described recently, and columns of mercurated dextran or dihydroxyborylcellulose have been used to fractionate nucleotide mixtures. Electrophoresis on polyacrylamide gels has been advocated as a rapid method for desalting and fractionating mixtures of oligonucleotides. ... 

Diethylaminoethyl-ceUulose Reaction product from epichlorohydrin, triethanolamine and alkaM-cellulose Aminoethyl-cellulose Carboxymethyl-cellulose Phosphorylated cellulose Polyethyleneimine-impregnated cellulose Polyphosphate-impregnated cellulose... 

ACPA azobis(4-cyanopentanoic acid) AIBN azobis isobutyronitrile) BPO benzoyl peroxide DVB divinyl benzene, EGA 2-ethylcyano-acrylate HPC hydroxypropyl cellulose MMA methyl methacrylate PAAc polyacrylic acid PEI polyethyleneimine, PEO/PPO polyethylene oxide/polypyropylene oxide copolymer PVME polyvinylmethylether PVP polyvinylpyrrolidone K-30 DMSO dimethylsulfoxide PGA polyglutaraldehyde CMS chloromethylstyrene PMMA-g-OSA polymethylmethacrylate grafted oligostearic acid. 

Figure 6.15 The effect of adsorption of polyethyleneimines of different molecular weight on electrophoretic mobility of cellulose (PEI 10 = DP of 10 and PEI 500 = DP of500 figures in brackets are negative).

Use a sheet of polyethyleneimine cellulose (PEI Cellulose), 50 x 200 mm, for chromatography. Mark the start about 15 mm away of the shorter side with a pencil. Apply the sample with a pipet or syringe. If larger volumes have to be used, apply them in several portions and/or a short line. 

Polyethyleneimine adsorbed to cellulose or weakly phosphorylated cellulose... 

Chen ZA, Deng MC, Yong C, He GH, Ming W, and Wang JD. Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications. J. Membr. Sci. 2004 235 73-86. 

A variety of powerful methods is available for fractionating short oligonucleotides. Work on nucleotide sequence analysis of small RNA molecules (n=80 or 120) and more recently viral RNA molecules (n= 3000) and even ribosomal precursor RNAs (up to 45 s) (Holley et al. 1965a Brownlee 1972 Maden et al. 1972) has stimulated the development of these procedures. They can also be used for synthetic oligonucleotides (e.g. Hachman and Khorana, 1969). The methods depend largely on chromatography and electrophoresis on filter papers, diethylaminoethyl(DEAE) paper, cellulose acetate, thin layers of cellulose or polyethyleneimine (PEI)-cellulose, or columns. 

C. Preparations and applications of polyethyleneimine- 13. immobilized cellulose fibers for endotoxin removal. 

Several functional groups have been used to obtain cellulose anion exchangers [aminoethyl (AE), diethyl-aminoethyl (DEAE)], or cation exchangers [car-boxymethyl (CM), phosphate (P)] for thin-layer chromatography. PEI cellulose is not a chemically modified cellulose, but a complex of cellulose with polyethyleneimine. These cellulose exchangers are particularly useful for the separation of proteins, aminoacids, enzymes, nucleobases, nucleosides, nucleotides, and nucleic acids. 

In this section, we discuss about the screen printed electrode (SPE) based AChE sensors for the selective determination of OP and CA pesticides. In the past decades, several attempts were made by the researchers to develop SPE based pesticide sensors, where the enzyme AChE was immobilized either directly onto the electrode or above other matrices incorporated SPE surfaces. Both approaches resulted in the good, rapid detection of OP and CA pesticides. Earlier, Hart et al. employed AChE/SPE to detect OP and CA pesticides [21], They measured the enzyme activity from the rate of hydrolysis of acetylthiocholine iodide. Three polymers such as hydroxyethyl cellulose, dimethylaminoethyl methacrylate, and polyethyleneimine were used as enzyme immobilization matrices. Initially, electrodes were exposed to drops of water or pesticide solution, dried and their activity was screened after 24 h. They found that, when the enzyme matrix was hydroxyethyl cellulose, electrode activity inhibited both by water as well as by pesticides. While with co-polymer matrix, a significant response towards pesticides alone was observed. Further, the long-term storage stability of electrodes was highest when the enzyme matrix consisted of the co-polymer. The electrodes retained their activity for nearly one year. In contrast, the electrodes made of hydroxyethyl cellulose or polyethyleneimine possess less stability. 

Sodium tripolyphosphate Polyethyleneimine Hydrox5 propyl cellulose... 

POLY(ETHYLENEIMINE) or POLYETHYLENEIMINE (26913-06-4) Combustible liquid (flash point 207°F/98°C). Violent reaction with strong oxidizers, strong acids. Incompatible with organic anhydrides, acrylates, alcohols, aldehydes, alkylene oxides, substituted allyls, cellulose nitrate, cresols, caprolactam solution, chlorine oxyfluoride, epichloro-hydrin, ethylene dichloride, isocyanates, ketones, glycols, nitrates, phenols, vinyl acetate. Exothermic decomposition with maleic anhydride. Increases the explosive sensitivity of nitromethane. Attacks aluminum, copper, magnesium, zinc, and other nonferrous metals. 

Al, aluminum oxide BA, boric acid C, cellulose CW, Carbowax 4(X) DEAE, diethylaminoethyl DMF, dimethylformamide DMSO, dimethylsulfoxide ECTEOLA, ethanolomine-epichlorhydrin EG, ethylene glycol F, formamide KC, Whatman KC reversed phase plates KG, kieselguhr OA, oxalic acid P, polyamide PEG, polyethylene glycol 4(X) PEI, polyethyleneimine PG, propylene glycol PO, paraffin oil POEG, polyoxyethylene glycol 1000 SG, silica gel SO, silicone oil TD, tetradecane Un, undecane. 

The final compounds are then isolated by any of the fast selective procedures available. These include thin-layer chromatography (tic) on polyethyleneimine (PEI) cellulose plates, slab preparative gel electrophoresis, and hplc on Per-maphase AAX or Partisil lOSax column. 

Entrapment in polyacrylamide gel over a platinum grid matrix Glutaraldehyde-mediated reaction with a nylon-polyethyleneimine copolymer Reaction with macroporous aminated silo chrome coated with an acrolein-vinylpyridine copolymer Adsorption onto tannin coupled to amino hex y 1-cellulo se Entrapment in cellulose fibres Carbodi-imide-mediated reaction with chitosan... 

An ATPase assay is performed by monitoring ADP formation using [ot- P] ATP (400 Ci/mmol, Amersham Pharmacia Biotech) on polyethyleneimine (PEI) cellulose thin-layer sheets (TLC sheet, Polygram cel 300 PEI, Macherey-Nagel, Germany), based on the procedure reported previously. ... 

Derived or coated celluloses are also available. These include acetylated, diethylaminoethyl (DEAE), polyethyleneimine (PEI). More on these will be discussed in the section Ion Exchange Layers below. 

---