Cellulose fibers polyethyleneimine immobilization

C. Preparations and applications of polyethyleneimine- 13. immobilized cellulose fibers for endotoxin removal. 

Polyethyleneimine (PEI) (Scheme 3) was immobilized onto cellulose fibers by a crosslinking agent glutaraldehyde (21). 10-60 wt% of PEI was attached by varying the mass ratio of PEI to cellulose. The cellulose-PEI fibers with 20-60 wt% of PEI adsorbed up to 10 mg endotoxin per g of modified fibers and could remove 90-100% endotoxin from 500 ng/ml endotoxin solution at pH 7.0 and ionic strengths lower than 0.4. In addition, the cellulose-PEI fibers with 20 wt% PEI showed selective removal activity toward endotoxins from various protein solutions at pH 7 and 0.05 ionic strength. This selective adsorption was due to simultaneous effects of cationic properties of amino groups and... 

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