Cells colonies

DL-CFU Dendritic cell/Langerhans cell colony forming DLE Discoid lupus erythematosus DMARD Disease-modifying antirheumatic drug... 

ES cell colony which was formed after ES cell colony and derived recovering from the PMBV/PVA hydrogel, (differentiated) cells which... 

Blazak, W.E, Steward, B.E., Galperin, I., Allen, K.L., Rudd, C.J., Mitchell, A.D. and Caspary, W.J. (1986). Chromosome analysis of triflourothymidine-resistant L5178Y mouse lymphoma cells colonies. Environ. Mutagen. 8 229-240. 

Granting that precise mechanisms responsible for the characteristic anticancer activity and mammalian toxicity of vinblastine, vincristine, and related compounds have not been rigorously established, it nevertheless is important to describe representative biological and biochemical actions of the drugs that may have mechanistic pertinence. The susceptibility to mitotic spindle dissolution of cell lines with 100-fold differences in sensitivity to vinblastine has been investigated 14). There was an excellent correlation between drug concentrations required to produce inhibition of cell colony formation and those required to dissolve mitotic spindles. It is noteworthy that effects on the mitotic spindle of vinblastine occur very rapidly and can be detected within 30 sec. 

Numerous methodologies have been developed. Generally, in vitro methods used to show the anticancer effect of compounds are based upon the determination of the number of viable cells or cell colonies present in a treated population of cells relative to the number of cells or colonies present in an untreated... 

Compound 2 inhibited murine and human bone marrow cell colony formation with and ID50 of 0.1-1 pg/ml, with complete inhibition occurring at 10-100 pg/ml. It was found to be more potent than vinblastine or taxol, with an IC50 of 0.23 nM against human ovarian cancer and colon cancer cell lines. Furthermore, dolastatin 10 was shown to be powerfully effective at binding to tubulin, inhibiting polymerization and it also non-competitively inhibits the binding of vinca alkaloids to tubulin,... 

Schulze, D.H. Kelsoe, G. (1987). Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. J. Exp. Med. 166, 163-172. 

Table 1 Different morphotypes reported for the six Phaeocystis species recognized to date Flagellates with scales, threads and stars Flagellates with scales Flagellates without scales, threads and stars Same size range as colonial cells Colonial cells...

Possible grazing from krill inducing FP export, downwelling of Phaeocystis cell/colonies/re-mains through deep physical mixing processes or nutrient-related flocculation and export of senescent Phaeocystis spp. mucus may induce episodic Phaeocystis flux events. 

Fig. 2 Results of surficial material collected from shallow fjord sediments experiment. Treatment key ArtSW artificial seawater AutoC autoclaved fjord water Ml- 0.8 pm filtered water from mesocosm 1 ( = no nutrients added) M2- 0.8 pm filtered water from mesocosm 2 (N03 and P04 added), (a) Abundance of P. pouchetii colonies (colonies ml-1) histograms represent colony estimates for each replicate bottle, (b) Colony diameter (pm) error bars represent standard deviations of estimates within each bottle calculated from triplicate counts, (c) Cell density (cells colony-1) error bars represent standard deviations of estimates within each bottle calculated from triplicate counts...

Fig. 3.5. Scatter assay. In the illustrated example, B16 melanoma cell colonies have been allowed to grow from few cells either in the absence (control) or in the presence (40 ng/ml) of HGF/SF. After fixation and staining with crystal violet, representative colonies have been photographed.

The continuous measurement of histamine released from rat basophilic leukaemia cells has been recently reported by Niwa [190], who coupled a microdialysis probe with a histamine oxidase-based carbon electrode obtained by coating the enzyme on the top of an Os-gel-HPR layer. For the exclusion of electrochemically active compounds, they suggested an overcoating of Nafion. In addition, a sensor using micro-machining techniques has been fabricated by the authors and apphed to the monitoring of histamine release from a small mast cell colony. 

The problem of multiple epitopes in an antigen can be overcome by using monoclonal antibodies produced in vitro, a technique first described in 1975 by Milstein and Kohler [6] who received the Nobel Prize for their revolutionary achievement. Antibody-secreting hybridoma cell colonies are cloned by means of limiting dilution, so that only one antibody-producing cell (clone) remains in each well. From this single parental cell, all the progeny secrete the same... 

However, if the net is equipped with a suitable flow meter or if the volume of water filtered tlirough the net is carefully noted, accuracy can be improved. Fig. 5 shows a typical result of aparticle size analysis of a netplankton sample of reservoir water during a bloom of dominant Dmtoma and Asterionella. Within a particle size range 7.0—30.0 jUm, different maxima in the particle size distribution were observed caused by the presence of both individual cells and of 2—5 celled colonies. A correlation coefficient of = 0.67 was found between the netplankton count by microscopy and the netplankton count by particle counter (cf. Fig. 6). 

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