Broth peptone yeast extract

Acetic acid is produced from complex basal medium (eg., peptone-yeast extract broth). 

COMPLEX BASAL MEDIUM CATABOLISM (E.G., PEPTONE YEAST EXTRACT BROTH)... 

Anaerobiospirillum succiniciproducens is a strict anaerobe and grows at an optimal temperature of 39 °C. A typical fermentation medium contains dextrose, peptone, yeast extract, and salts. The optimal pH range for this organism was determined to be between 5.8 and 6.4. This organism can produce approximately 30 g of succinate/1 fi-om a starting glucose concentration of 50 g/1. Calcium hydroxide is added to produce a calcium succinate product, which can be precipitated fi-om the broth (Datta 1992). 

Natural media are those used on the basis of experience and not on the basis of exact knowledge of their composition and action. Natural or complex media usually contain peptones, beef extract, or yeast extract. When a solid medium is desired, a solidifying agent such as gelatin or agar may be incorporated into the medium. Examples of a relatively simple liquid and a solid medium that support the growth of many common heterotrophs are nutrient broth and nutrient agar. Their composition is as follows ... 

Nutrient broth 3 g of beef extract, 5 g of peptone, 5 g of yeast extract, and water to make 1 L. 

The first-stage inoculum medium consisted of Emerson broth [R. L. Emerson et al., J. Bacteriol, 52, 357 (1946)] 0.4% peptone, 0.4% sodium chloride, 0.25% yeast extract and 1% glucose pH 7.0 flasks containing the above medium were inoculated with 1 % of the spore suspension described above. The inoculated flasks were incubated for 30 hours at 28°C on a reciprocating shaker set at 65 r.p.m. (4 inch stroke). 

Yeast strains are grown to saturation in YPED broth, diluted 1 100 in YEPA [1% (w/v) yeast extract, 2% (w/v) peptone, 2% (w/v) potassium acetate] and... 

OT Brothy, cooked meat, meat extract, peptone, yeast broth. ... 

Note YPG media (0.2% yeast extract, 1.0% peptone, and 2.0% glucose) MSM, molasses salt medium (0.2% yeast extract and molasses as carbon source) PDB, potato dextrose broth (20% potato extract and 2% dextrose) YM media (0.3% yeast extract, 0.3% malt extract, 0.5% polypeptone, and 1.0% glucose) AIF, alkali insoluble fraction DA, degree of acetylation DDA, degree of deacetylation DP, degree of polymerization M, molecular weight SmF, submerged fermentation SSF, solid-state fermentation. 

In order to illustrate carbohydrate analysis in fermentation broths [303], a yeast and a bacterial fermentation broth were used Saccharomyces cerevisiae in a YPD broth (Yeast Extract-Peptone-Dextrose) and Escherichia coli E. coli) in a LB broth (Luria-Bertani). These are common eucaryotic and procaryotic fermentation systems, respectively. They represent a great challenge for most separation... 

Aseptically decant the broth, and suspend the pellet in a minimal amount of a sterile basal broth that contains 1% w/v peptone, 0.25% w/v yeast extract, 0.01% w/v Tween 80, and 0.2g/L calcium pantothenate. 

The precultuie medium was 13 g/1 nutrient broth, 10 g/1 peptone, and 10 g/1 yeast extract, sterilized at 121 °C for 15 min and prepared with a single colony withdrawn from the slants and maintained for 48 hr at an incubator shaker with a temperature of 30°C and velocity of 150 rpm. In all the experiments 100 ml of sterile Erlenmeyer flask were charged with a 50 ml of pre-crrltrrre. 

The assay inoculum is prepared by transferring the organism to broth with the following composition peptone 5.0 g., yeast extract 1.5 g., beef extract 1.5 g., sodium chloride 3.5 g., glucose 1.0 g., dipotassium phosphate 3.68 g., potassium dihydrogen phosphate 1.32 g., and distilled water 1 liter. The pH of the broth is adjusted to 7.0. The inoculated broth is incubated at 37 C. for 16-18 hours. This inoculum is prepared daily. 

Culture media. The basis of all sterility test media is a nutrient broth containing TO per cent w/v peptone, 0 2 per cent meat extract (Lab-Lemco or other suitable brand), 0 2 per cent yeast extract (Yeastrel or other suitable brand), 0 25 per cent sodium chloride and 01 per cent glucose. Alternatively, a medium made from either the papaic or the tryptic digestion of lean meat (ox heart is preferred) may be used. 

These authors have given several procedures for the preparation of the antibiotic from cultures of M. verrucaria S 118. One liter of medium contained 20 g of glucose, 2 g of malt extract, 2 g of yeast extract, 2 g of peptone, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate heptahydrate and 0.2 g of ferrous sulfate heptahydrate. After 63 hours, 501 of broth was extracted with ethyl acetate, and the extract concentrated to 41. The crude product was washed with dilute sodium hydroxide solution and water and recrystalhzed to give 2.31 g of verrucarin A contaminated with small amounts of verrucarin B. Silica gel... 

Escherichia coli K-12 CE 1237 and CE 1241 were a gift from Ben Lugtenberg (State University of Utrecht) and were cultured aerobically at 37 C in yeast broth consisting of 1% peptone, 0.7% yeast extract, 0.5% NaCl and 0.1% NaH2P04 2H20. 

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