Broth peptone

To 100 ml of a sterile nutrient broth (composed of Bacto-beef extract, 3 g Bacto-peptone, 5 g per liter of tap water) in a 300 ml flask is added one loopful of the incubated culture and the broth mixture is further incubated for 24 hours at 28°C on a shaking machine. 

The pathogenicity of DRB to crop plants has been shown to be host-specific (35) and thus is conceivably linked to root exudation. Alstrom (169) found that the pathogenicity of two isolates of Pseudomonas was determined by the major components of the broth culture in which they were applied to bean seedlings. Both isolates were pathogenic to bean seedlings when the broth contained sucrose and peptone or sucrose and yea.st extract. When the broth contained sucrose alone, one isolate was pathogenic and the other was not. Neither isolate was pathogenic when the broth contained yea.st extract or peptone alone (169). 

Sarafloxacin M. ramannianus Cultures grown in sucrose-peptone broth were dosed with sarafloxacin 260 uM 59% of the starting material remained. N-acetylsarafloxacin desethylene-N- acetylsarafloxacin [37]... 

Natural media are those used on the basis of experience and not on the basis of exact knowledge of their composition and action. Natural or complex media usually contain peptones, beef extract, or yeast extract. When a solid medium is desired, a solidifying agent such as gelatin or agar may be incorporated into the medium. Examples of a relatively simple liquid and a solid medium that support the growth of many common heterotrophs are nutrient broth and nutrient agar. Their composition is as follows ... 

Nutrient broth 3 g of beef extract, 5 g of peptone, 5 g of yeast extract, and water to make 1 L. 

To 100 ml of a sterile nutrient broth (composed of Bacto-beef extract, 3 9 Bacto-peptone, 5 9 per liter of tap water) in a 300 ml flask is added one loopful of the incubated culture and the broth mixture is further incubated for 24 hours at 28°C on a shaking machine. The broth culture so obtained is employed as an inoculum (1%). Into each often flasks containing 100 ml of sterile nutrient broth is added 1 ml of the inoculum. The flasks are agitated on a rotary shaker for 8 hours at 28°C at 240 strokes per minute. After this growth period, a solution of 25 mg of 16p-methylcortisonein 0.5 ml of methanol is aseptically added to each flask which in turn is reshaken and incubated for an additional 24 hours. The final pH is 7.8. 

The first-stage inoculum medium consisted of Emerson broth [R. L. Emerson et al., J. Bacteriol, 52, 357 (1946)] 0.4% peptone, 0.4% sodium chloride, 0.25% yeast extract and 1% glucose pH 7.0 flasks containing the above medium were inoculated with 1 % of the spore suspension described above. The inoculated flasks were incubated for 30 hours at 28°C on a reciprocating shaker set at 65 r.p.m. (4 inch stroke). 

These included peptone water (bacteriological peptone, Oxoid 1.0%, Analar NaCl 0.5%, pH 12-1 A), nutrient broth (Oxoid), and Mueller-Hinton broth (Oxoid). 

The fungus Helirastylum piriforme ATCC 8992 is cultivated for 3 d at 28 "C in a medium (pH 6.8-7.0) consisting of 3.5 % glucose. 2% peptone and 0.3 % corn steep liquoT in tap water, on a rotary shaker. The wet mycelium harvested from the culture broth (100 ml) is suspended in 100 mL of deionized water and 50 mg (0.13 mmol) of finely divided lithocholic acid (3) are added. The mixture is incubated for 2 d at 28 JC on a shaker. Extraction of the incubation mixture with EtOAc yields a mixture of hydroxylation products. The principal component 4 is purified by column chromatography on silica gel and isolated by crystallization from EtOAc and then from acetone yield 20 mg ( 40%) mp 171.5- 172.5 C. 

The effects of ampicillin-induced spheroplast formation on the production of molecular hydrogen by Escherichia coli carrying out fermentation in a lactose-peptone broth with an osmolality of 342 mosmol/1 was investigated previously... 

The presence/absence procedure was based on the ISO method for the detection of Salmonella (ISO 6579) which is summarised as follows (1) pre-enrichment in Buffered Peptone water (incubation time (18 2) h at (37 1)°C) and (2) selective enrichment in broth of own choice (incubation time and temperature according to own procedure). The detailed procedure is described elsewhere [37]. Each laboratory determined the presence or absence of Salmonella in 50 capsules. Four of these individually identified capsules were negative control capsules. The numbers of these capsules were unknown to the laboratories at the time of analysis. For the presence/absence procedure, all capsules showing typical colonies on the isolation agar were subjected to a confirmation for Salmonella. At least two colonies per capsule were used for this confirmation. All colonies (>1000 colonies) tested by the laboratories gave a positive Salmonella identification. The type of confirmation test used is described elsewhere [37]. 

The plate counts at To were estimated by diluting the neat broth culture 1 in 10 with Neutralised Peptone Water (NPW) containing per L 1.0 g Bacteriological Peptone (Oxoid, L37), 8.8 g Sodium Chloride (May Baker), 3.0 g Amisol 910 (Degussa) and 30.0 g Tween 80 (BDH, 560234H) and then decimally with Phosphate Buffered Saline (Oxoid). For biocide treated samples 1 mL was diluted in 9 mL of NPW, mixed and allowed to stand for 5 min (for neutralisation of the biocide). The solution in NPW was diluted decimally (0.1 mL in 0.9 mL) in PBS. Appropriate dilutions (0.1 mL) were plated out on Tryptone Soya Agar Plates (bioMerieux) and incubated at 37 °C for 24 h. 

The broth dilution method was used in these studies. Pseudomonas organisms were cultivated in I per cent peptone, 3 per cent NaCi medium, and Dipl, pneumoia. Sir, pyo nes and CoU. Dipktk riae were cultivated on heart infusion broth containing 0 5 per cent glucose. All other bacteria were cultivated on heart infusion broth. F gi were grown in Sabouraud s medium. 

The first issue in bioburden determination that merits validation is the choice of fluid used in preparatory stages of removal of microorganisms from devices and for suspending, dissolving, and diluting dosage forms. Phosphate buffer pH 7.2. buffered sodium chloride-peptone solution pH 7.0, and lactose broth are recommended in the various compendia. Saline, Rtnger s solution, and... 

The fungal strain (104 spores) was grown in a glucose-peptone broth (pH 5.0) for 144 h at 25° C, and the growth was evaluated by measuring the weight of dried mycelium. Symbols D, without HMTP and O, with 200 pg/ml HMTP. The arrow indicates the time at which the corresponding amounts of HMTP were added. 

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