Broth yeast extract

Nutrient broth yeast extract vegetable oil, H. heliothidis 4 weeks 25°C 107250 ml flask Wouts (1981)... 

In order to illustrate carbohydrate analysis in fermentation broths [303], a yeast and a bacterial fermentation broth were used Saccharomyces cerevisiae in a YPD broth (Yeast Extract-Peptone-Dextrose) and Escherichia coli E. coli) in a LB broth (Luria-Bertani). These are common eucaryotic and procaryotic fermentation systems, respectively. They represent a great challenge for most separation... 

Figure 7 Induction of cutinase activity in Pseudomonas putida cultures in nutrient broth-yeast extract with or without cutin hydroiysate (1 and2) and with cutin (3). Tracing A indicates release of iabeied monomers from H-labeled cutin.

A seed culture of S. cerevisiae ATCC 24860 (American Type Culture Collection, Manassas, VA, USA) was grown in a media of 5g glucose, and 0.5 g yeast extract, respectively, 1.5 g KH2P04 and 2.25 g Na2P04 phosphate buffer up to a total volume of distilled water, 500 ml. The media was sterilised at 121 °C for 15 min. The stock culture of the microorganisms was transferred to the broth media for preparation of seed culture. 

Luria-Bertani (LB) broth tryptone (35 g) yeast extract (17.5 g)... 

Malt extract (1 g), yeast extract (1 g) and magnesium sulfate heptahydrate (0.012 g) were dissolved with water and the volume was adjusted to 100 mL with tap water. The culture medium (30 mL) was placed in a 500 mL shaking-flask with a cotton plug and sterilized (121 °C, 20 min). The seed culture broth was transferred to a 500 mL shaking-flask containing 30 mL of the culture medium. Cultivation was carried out for 48 h at 28 °C and 115 strokes per minute. 

Isolation of Plasmid Enriched DNA. The three strains of B. megaterium were cultured in yeast-soy broth (4% Bacto-yeast extract and 1.6% Bacto-soytone from Difco Laboratories, Detroit, pH 7.2). Plasmid DNA from each strain was isolated and purified by cesium chloride-ethidium bromide equilibrium density centrifugation following the procedure of Carlton and Brown ( ). Subsequent gel electrophoresis (0.5% agarose) revealed the presence of some chromosomal DNA contamination. 

LB broth 10 g/L Bacto-tryptone, 5 g/L Bacto-yeast extract, 10 g/L NaCl, adjusted to pH 7.0 with NaOH and sterilized by autoclaving. 

Natural media are those used on the basis of experience and not on the basis of exact knowledge of their composition and action. Natural or complex media usually contain peptones, beef extract, or yeast extract. When a solid medium is desired, a solidifying agent such as gelatin or agar may be incorporated into the medium. Examples of a relatively simple liquid and a solid medium that support the growth of many common heterotrophs are nutrient broth and nutrient agar. Their composition is as follows ... 

Nutrient broth 3 g of beef extract, 5 g of peptone, 5 g of yeast extract, and water to make 1 L. 

THY broth Todd-Hewitt broth (Beckton Dickisnson, MD, USA) supplemented with 1% Bacto yeast extract (Beckton Dickisnson, MD, USA). 

A large amount of rice bran caused excessive fungal growth rather than enhance fumaric acid production. In addition, we could produce fumaric acid without the addition of zinc and iron. Fungal culture broth containing approx 25 g/L of fumaric acid was directly employed for succinic acid conversion. The amount of glycerol and yeast extract required for succinic acid conversion was reduced to 70 and 30%, respectively, compared with the amounts cited in previous studies. 

The first-stage inoculum medium consisted of Emerson broth [R. L. Emerson et al., J. Bacteriol, 52, 357 (1946)] 0.4% peptone, 0.4% sodium chloride, 0.25% yeast extract and 1% glucose pH 7.0 flasks containing the above medium were inoculated with 1 % of the spore suspension described above. The inoculated flasks were incubated for 30 hours at 28°C on a reciprocating shaker set at 65 r.p.m. (4 inch stroke). 

Two thousand parts by volume of an aqueous culture medium (pH 7.2) comprising 0.5% of glycerol, 0.5% of polypeptone, 0.5% of yeast extract and 0.3% of meat extract is inoculated with Escherichia coli Rll (IFO-13560). The medium is incubated at 37°C under aeration for 18 h. The culture broth is subjected to centrifuge to recover 4.4 parts of wet cells. The cells are suspended into 17.6 parts by volume of 0.05 M phosphate buffer (pH 7.0). The suspension is subjected to ultrasonic oscillation (Kaijo Denki Co., Ltd. T-A-4201, 4280-type, 2A) to disintegrate the cells, followed by removing the debris (insoluble materials) by centrifugation, whereby 17 parts by volume of crude enzyme solution is obtained. 

There are a number of media available which are not based on a detailed investigation of growth requirements, but rather include crude mixtures of nutrients added to promote cell growth. These include lactalbumin hydrolysate (Appendix 1 Table 9) or yeast extract (Appendix 4) to provide an inexpensive source of amino acids or vitamins. Thus Melnick s monkey kidney media A and B (Melnick, 1955) contain lactalbumin hydrolysate and calf serum in Hanks and Earle s BSS, respectively. Chick embryo extract and tryptose phosphate broth (Appendix 1, Tables 11 and 12) are also used occasionally and their use is referred to where appropriate throughout the book. Mitsuhashi and Maramorosch mosquito cell medium contains lactalbumin hydrolysate, yeast extract and foetal calf serum in a specially developed saline (Mitsuhashi and Maramorosch, 1964 Singh, 1967). 

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