Reciprocating shaker

The pH of the medium thus prepared is about 6.8. An 8 ml portion Is measured into an 8 inch 8rewer tube and sterilized at 120°C for 20 minutes. The sterilized medium is than inoculated with 0.5 ml of an aqueous spore suspension of a strain of S. aureofaciens capable of producing chlorodemethyltetracycline, such as S-604, containing approximately 40-60 million spores per milliliter. The inoculated medium is incubated for 24 hours at 28°C on a reciprocating shaker operated at 110 cycles per minute. 

The seed flash was incubated for 24 hours at 32°C on a reciprocating shaker after which it was used as an inoculum for a 20 liter seed fermenter in the amount of approximately 5%. the 20 liter seed fermenter contained a sterile medium consisting of ... 

The flasks are then placed on a reciprocating shaker (120 one and one-half inch cycles per minute) and mechanically shaken at 25°C for 3 days. The contents of the flasks are then pooled and, after the pH of the culture is adjusted to about 4 0.2 with sulfuric acid, filtered through Seitz filter pads to separate the mycelium from the fermented medium. 

UDPGA (2 mL, 100 mM) and freshly thawed microsomal fraction (1.5 mL, 20 mg protein/mL) were added to a 25 mL glass tube containing tris-HCl solution(2 mL), 1 mL CaCl2 (1 mL), BSA (1 mL), DTT (50 microL) and H2O (1.45 mL). The mixture was mixed well, sealed and pre-incubated in a reciprocal shaker for 2 min at 35 °C. Freshly prepared 1 mL substrate solution was added to the reaction mixture, which was subsequently sealed with mbber cap, purged with N2 gas and left in the reciprocal shaker (130 rpm) for 6-8 h at 35 °C. 

Prior to analysis, the exposed glass fiber filters were carefully cut into 4 x 10 cm segments taken from the central area of the filter. The segments were further cut into narrow strips ( 5 mm wide x 4 cm long) which were placed collectively in a 20 ml vial containing 10 ml acetone. The vial was placed in an ultrasonic bath for 30 min or shaken on a reciprocal shaker at 120 cpm for 2 hrsl An aliquot of the supernatant was withdrawn for analysis. 

Procedure. Weigh 20 g of air-dry soil sieved to 2 mm into a 250-ml wide-mouth high-density polyethylene screw-cap bottle. The square type bottles fit best the square box of the reciprocating shaker. Add 100 ml 1 M KCI and shake for 15 min. Transfer all the suspension to a filter funnel holding a Whatman No. 6 paper, and collect the filtrate. When leaching has ceased, add two successive 50-ml aliquots of 1 M KCI. Combine the leachates from the total addition of 200 ml (some will be retained in the soil) and mix. 

Procedure. Transfer a 20-ml level scoop of air-dry soil sieved to s2 mm to a 125-ml wide-mouth HDPE screw-cap bottle, add 50 ml 0.01 M calcium sulphate solution, and shake for 15 min on a reciprocating shaker (MAFF/ADAS use 275 strokes per minute. Northeastern US use 200 oscillations per minute). Filter through a 125-mm Whatman No. 2 filter paper into... 

Procedure (extraction). Transfer 5 ml (scoop filled and struck off level without tapping) of air-dry soil, sieved to 2 mm into a bottle (e.g. wide-mouth, square HDPE). Add 100 ml of sodium bicarbonate reagent, pH 8.50, cap the bottle and shake on a reciprocating shaker, at approximately 275 strokes of 25 mm length per minute, for 30 min at 20°C. Filter a portion immediately through a Whatman No. 2 filter paper, rejecting the first few millilitres of filtrate. Carry out a blank determination. 

Procedure (extraction). Weigh 10 g air-dry soil sieved to s2 mm (10 mesh) into a 50-ml conical flask. Add 25 ml of calcium phosphate extractant (50 ml for peat or compost) and shake on a reciprocating shaker (at approximately 200-275 oscillations of 25 mm per minute) for 30 min. If the presence of sol-... 

Procedure (extraction). Transfer 2.5 g air-dry soil, 2 mm mesh size, into a 250 ml polypropylene screw-cap centrifuge bottle/tube and add 100 ml acetic acid - 8-hydroxyquinoline reagent. Cap the tube and shake overnight (17 h) on a reciprocating shaker, at approximately 275 strokes of 25 mm length per minute at a constant temperature (20°C). Centrifuge for 15 min at 2800 rpm and remove an aliquot for the determination of acid extractable inorganic phosphorus (a). 

Figure 6. Transglycosylation activity of /3-glucosidase. The reaction mixture initially contained 3% cellobiose, 0.0009% (3-glucosidase, and 0.05M sodium acetate buffer, pH 5 0. Incubation was carried out at 45°C in a reciprocating shaker. Each sample was boiled for five minutes and analyzed by HPLC (Gt = glucose, G2 = cellobiose, Gs = trisaccharides.)...

Many kinetic studies investigating reactions on soil constituents have used batch techniques. The traditional batch or tube technique involves placing an adsorbent and the adsorptive in a vessel such as a centrifuge tube. The suspension is stirred or agitated using a reciprocating shaker. Then the suspension is usually centrifuged or filtered to separate a clear supernatant solution for subsequent analysis. 

A mycelial mat (2x2 mm) was incubated in wheat bran medium for 6 d at 28°C. Three Erlenmeyer flasks were used for estimation of one strain. The enzyme solution and the substrate were incubated for 2 h at 4°C using a reciprocal shaker (125 strokes/min). The amount of reducing sugar was measured using DNS. 

The first-stage inoculum medium consisted of Emerson broth [R. L. Emerson et al., J. Bacteriol, 52, 357 (1946)] 0.4% peptone, 0.4% sodium chloride, 0.25% yeast extract and 1% glucose pH 7.0 flasks containing the above medium were inoculated with 1 % of the spore suspension described above. The inoculated flasks were incubated for 30 hours at 28°C on a reciprocating shaker set at 65 r.p.m. (4 inch stroke). 

Ten litres 0.5 M K2S04 are prepared by shaking on a reciprocal shaker about 9.5 1 distilled H20 and K2S04 (AR grade) (871 g) in a 10 1 aspirator for about two hours. When the K2S04 has dissolved completely, the volume is adjusted to 10 1 with distilled water. Three replicate samples of each sieved (< 2 mm) soil (40%-50% WHC), usually 50 g (oven-dry basis) are weighed into glass... 

Horizontal reciprocal shaker (orbital shakers are less effective). 

Adherent cells Cells are incubated with 300 pL LM/well for 2-4 h at 4°C on a reciprocal shaker set at approx 30 oscillations/min. Subsequently, the LM is aspirated away and the cells washed at least 4 times with 1 ul./we 11 4°C BM and twice in 4°C PBS. The PBS is removed by aspiration and 400 pL 0.2 M NaOH is added to each well. The lysed cells in NaOH are transferred to y counter tubes. Each well is rinsed with 400 pL H20, the rinse added to the lysate, and the 125I activity counted. (See Note 1 for data normalization.)... 

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